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DDX58 (NM_014314) Human Untagged Clone
CAT＃: SC115033
DDX58 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58)
CNY 10,168.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RIG-I; RIG1; RIGI; RLR-1; SGMRT2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_014314 edited
GAATTCGGCACGAGGAAAGTTCCTATGCAGCTCCGCCTCGCGTCCGGCCTCATTTCCTCG GAAAATCCCTGCTTTCCCCGCTCGCCACGCCCTCCTCCTACCCGGCTTTAAAGCTAGTGA GGCACAGCCTGCGGGGAACGTAGCTAGCTGCAAGCAGAGGCCGGCATGACCACCGAGCAG CGACGCAGCCTGCAAGCCTTCCAGGATTATATCCGGAAGACCCTGGACCCTACCTACATC CTGAGCTACATGGCCCCCTGGTTTAGGGAGGAAGAGGTGCAGTATATTCAGGCTGAGAAA AACAACAAGGGCCCAATGGAGGCTGCCACACTTTTTCTCAAGTTCCTGTTGGAGCTCCAG GAGGAAGGCTGGTTCCGTGGCTTTTTGGATGCCCTAGACCATGCAGGTTATTCTGGACTT TATGAAGCCATTGAAAGTTGGGATTTCAAAAAAATTGAAAAGTTGGAGGAGTATAGATTA CTTTTAAAACGTTTACAACCAGAATTTAAAACCAGAATTATCCCAACCGATATCATTTCT GATCTGTCTGAATGTTTAATTAATCAGGAATGTGAAGAAATTCTACAGATTTGCTCTACT AAGGGGATGATGGCAGGTGCAGAGAAATTGGTGGAATGCCTTCTCAGATCAGACAAGGAA AACTGGCCCAAAACTTTGAAACTTGCTTTGGAGAAAGAAAGGAACAAGTTCAGTGAACTG TGGATTGTAGAGAAAGGTATAAAAGATGTTGAAACAGAAGATCTTGAGGATAAGATGGAA ACTTCTGACATACAGATTTTCTACCAAGAAGATCCAGAATGCCAGAATCTTAGTGAGAAT TCATGTCCACCTTCAGAAGTGTCTGATACAAACTTGTACAGCCCATTTAAACCAAGAAAT TACCAATTAGAGCTTGCTTTGCCTGCTATGAAAGGAAAAAACACAATAATATGTGCTCCT ACAGGTTGTGGAAAAACCTTTGTTTCACTGCTTATATGTGAACATCATCTTAAAAAATTC CCACAAGGACAAAAGGGGAAAGTTGTCTTTTTTGCGAATCAGATCCCAGTGTATGAACAG CAGAAATCTGTATTCTCAAAATACTTTGAAAGACATGGGTATAGAGTTACAGGCATTTCT GGAGCAACAGCTGAGAATGTCCCAGTGGAACAGATTGTTGAGAACAATGACATCATCATT TTAACTCCACAGATTCTTGTGAACAACCTTAAAAAGGGAACGATTCCATCACTATCCATC TTTACTTTGATGATATTTGATGAATGCCACAACACTAGTAAACAACACCCGTACAATATG ATCATGTTTAATTATCTAGATCAGAAACTTGGAGGATCTTCAGGCCCACTGCCCCAGGTC ATTGGGCTGACTGCCTCGGTTGGTGTTGGGGATGCCAAAAACACAGATGAAGCCTTGGAT TATATCTGCAAGCTGTGTGCTTCTCTTGATGCGTCAGTGATAGCAACAGTCAAACACAAT CTGGAGGAACTGGAGCAAGTTGTTTATAAGCCCCAGAAGTTTTTCAGGAAAGTGGAATCA CGGATTAGCGACAAATTTAAATACATCATAGCTCAGCTGATGAGGGACACAGAGAGTCTG GCAAAGAGAATCTGCAAAGACCTCGAAAACTTATCTCAAATTCAAAATAGGGAATTTGGA ACACAGAAATATGAACAATGGATTGTTACAGTTCAGAAAGCATGCATGGTGTTCCAGATG CCAGACAAAGATGAAGAGAGCAGGATTTGTAAAGCCCTGTTTTTATACACTTCACATTTG CGGAAATATAATGATGCCCTCATTATCAGTGAGCATGCACGAATGAAAGATGCTCTGGAT TACTTGAAAGACTTCTTCAGCAATGTCCGAGCAGCAGGATTCGATGAGATTGAGCAAGAT CTTACTCAGAGATTTGAAGAAAAGCTGCAGGAACTAGAAAGTGTTTCCAGGGATCCCAGC AATGAGAATCCTAAACTTGAAGACCTCTGCTTCATCTTACAAGAAGAGTACCACTTAAAC CCAGAGACAATAACAATTCTCTTTGTGAAAACCAGAGCACTTGTGGACGCTTTAAAAAAT TGGATTGAAGGAAATCCTAAACTCAGTTTTCTAAAACCTGGCATATTGACTGGACGTGGC AAAACAAATCAGAACACAGGAATGACCCTCCCGGCACAGAAGTGTATATTGGATGCATTC AAAGCCAGTGGAGATCACAATATTCTGATTGCCACCTCAGTTGCTGATGAAGGCATTGAC ATTGCACAGTGCAATCTTGTCATCCTTTATGAGTATGTGGGCAATGTCATCAAAATGATC CAAACCAGAGGCAGAGGAAGAGCAAGAGGTAGCAAGTGCTTCCTTCTGACTAGTAATGCT GGTGTAATTGAAAAAGAACAAATAAACATGTACAAAGAAAAAATGATGAATGACTCTATT TTACGCCTTCAGACATGGGACGAAGCAGTATTTAGGGAAAAGATTCTGCACATACAGACT CATGAAAAATTCATCAGAGATAGTCAAGAAAAACCAAAACCTGTACCTGATAAGGAAAAT AAAAAACTGCTCTGCAGAAAGTGCAAAGCCTTGGCATGTTACACAGCTGACGTAAGAGTG ATAGAGGAATGCCATTACACTGTGCTTGGAGATGCTTTTAAGGAATGCTTTGTGAGTAGA CCACATCCCAAGCCAAAGCAGTTTTCAAGTTTTGAAAAAAGAGCAAAGATATTCTGTGCC CGACAGAACTGCAGCCATGACTGGGGAATCCATGTGAAGTACAAGACATTTGAGATTCCA GTTATAAAAATTGAAAGTTTTGTGGTGGAGGATATTGCAACTGGAGTTCAGACACTGTAC TCGAAGTGGAAGGACTTTCATTTTGAGAAGATACCATTTGATCCAGCAGAAATGTCCAAA TGATATCAGGTCCTCAATCTTCAGCTACAGGGAATGAGTAACTTTGAGTGGAGAAGAAAC AAACATAGTGGGTATAATCATGGATCGCTTGTACCCCTGTGAAAATATATTTTTTAAAAA TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_014314 unedited
ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGNAAAGTTCCTATGCAGCTCCGC CTCGCGTCCGGCCTCATTTCCTCGGAAAATCCCTGCTTTCCCCGCTCGCCACGCCCTCCT CCTACCCGGCTTTAAAGCTAGTGAGGCACAGCCTGCGGGGAACGTAGCTAGCTGCAAGCA GAGGCCGGCATGACCACCGAGCAGCGACGCAGCCTGCAAGCCTTCCAGGATTATATCCGG AAGACCCTGGACCCTACCTACATCCTGAGCTACATGGCCCCCTGGTTTAGGGAGGAAGAG GTGCAGTATATTCAGGCTGAGAAAAACAACAAGGGCCCAATGGAGGCTGCCACACTTTTT CTCAAGTTCCTGTTGGAGCTCCAGGAGGAAGGCTGGTTCCGTGGCTTTTTGGATGCCCTA GACCATGCAGGTTATTCTGGACTTTATGAAGCCATTGAAAGTTGGGATTTCAAAAAAATT GAAAAGTTGGAGGAGTATAGATTACTTTTAAAACGTTTACAACCAGAATTTAAAACCAGA ATTATCCCAACCGATATCATTTCTGATCTGTCTGAATGTTTAATTAATCAGGAATGTGAA GAAATTCTACAGATTTGCTCTACTAAGGGGATGATGGCAGGTGCAGAGAAATTGGTGGAA TGCCTTCTCAGATCAGACAAGGAANACTGGCCCAAAACTTTGAAACTTGCTTTGGAGAAA GAAGGAAACAGTTCAGTGAACTGTGGATTGTAGAGAAAGTATCAAAGATGTTGAAACAGA AGATCTTGCAGACTAGATGGGAACTTCTGACATACAGATTTTCTACCAAGAAGATTCCAG AATGCCGAATCCTTAGTGAGCATTCATGTTCACCCTTCAGAAGTGTCTGATCCAAACTGC ACAGCCCATTNAACCAGAAATACATTTACAGCCTGCCTTGCTGCTATGAAGGNAACACAC ATATATGGCTCCTACGGGNTGGGGAAAAACCTTGTTCACTGTTACTCGGACTCATCTAAA ATCCAAGG >OriGene 3' read for NM_014314 unedited
ACTATGAACCCGCGGCCGCAATCTAGNGATCAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTATTTTTAAAAAAATATTTTCACAGGGGTACAAGCGATCCATGATTATACC CACTATGTTTGTTTCTTCTCCACTCAAAGTTACTCATTCCCTGTAGCTGAAGATTGAGGA CCTGATATCATTTGGACATTTCTGCTGGATCAAATGGTATCTTCTCAAAATGAAAGTCCT TCCACTTCGAGTACAGTGTCTGAACTCCAGTTGCAATATCCTCCACCACAAAACTTTCAA TTTTTATAACTGGAATCTCAAATGTCTTGTACTTCACATGGATTCCCCAGTCATGGCTGC AGTTCTGTCGGGCACAGAATATCTTTGCTCTTTTTTCAAAACTTGAAAACTGCTTTGGCT TGGGATGTGGTCTACTCACAAAGCATTCCTTAAAAGCATCTCCAAGCACAGTGTAATGGC ATTCCTCTATCACTCTTACGTCAGCTGTGTAACATGCCAAGGCTTTGCACTTTCTGCAGA GCAGTTTTTTATTTTCCTTATCAGGTACAGGTTTTGGTTTTTCTTGACTATCTCTGATGA ATTTTTCATGAGTCTGTATGTGCAGAATCTTTTCCCTAAATACTGCTTCGTCCCATGTCT GAAGGCGTAAAATAGAGTCATTCATCATTTTTTCTTTGNACATGTTTATTTGTTCTTTTT CAATTACACCAGCATTACTAGTCAGAAGGAAGCACTTGCTACCTCTTGCTCTTCCTCTGC CTCTGGTTTGGATTCATTTGATGACATTGCCCACATACTCATAAAGGATGACAAGATTGC ACTGTGCAATGTCAATGCCTTCATCAGCAACTGGAGGTGGCATCAGAATATTTGGATCTC CACTGGGCTTGAATGCATCCATATACCTTCTGTGCCCGGAGGGNCNTNCCTGGGTTCTGA ATTGTTTTGCCCGTCCAGTCAATATGCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014314 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014314.2, NP_055129.2 |
| RefSeq Size | 4372 bp |
| RefSeq ORF | 2778 bp |
| Locus ID | 23586 |
| UniProt ID | O95786 |
| Domains | DEAD, helicase_C |
| Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2. [provided by RefSeq, Aug 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Retinoic acid induced gene-1 (RIG-I) associates with the actin cytoskeleton via CARD-dependent interactions
,Amitava Mukherjee, Stefanie A. Morosky, Le Shen, Christopher R. Weber, Jerrold R. Turner, Kwang Sik Kim, Tianyi Wang, and Carolyn B. Coyne,
J. Biol. Chem., Jan 2009; 10.1074/jbc.M807547200.
[DDX58]
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Essential role of the N-terminal domain in the regulation of RIG-I ATPase activity
,Peter Gee, Pong Kian Chua, Jirair Gevorkyan, Klaus Klumpp, Isabel Najera, David C. Swinney, and Jerome Deval,
J. Biol. Chem., Feb 2008; 10.1074/jbc.M706777200.
[DDX58]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217615 | DDX58 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) |
CNY 10,160.00 |
|
| RC217615L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), Myc-DDK-tagged |
CNY 12,560.00 |
|
| RC217615L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mGFP tagged |
CNY 9,030.00 |
|
| RC217615L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), Myc-DDK-tagged |
CNY 12,560.00 |
|
| RC217615L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mGFP tagged |
CNY 12,560.00 |
|
| RG217615 | DDX58 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) |
CNY 11,760.00 |
