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E2F7 (NM_203394) Human Tagged ORF Clone
CAT＃: RC217596
- TrueORF®
E2F7 (Myc-DDK-tagged)-Human E2F transcription factor 7 (E2F7)
ORF Plasmid: tGFP
"NM_203394" in other vectors (4)
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CNY 6,672.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217596 representing NM_203394
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTAAATTGTTTAACACTAAAAGACCTGATCAGCCCCAGGCAGCCCAGACTAGATTTTGCAGTTG AAGATGGGGAAAATGCACAAAAGGAAAATATATTTGTTGATCGATCAAGGATGGCCCCGAAGACTCCAAT AAAAAATGAACCAATTGATTTATCGAAGCAAAAAAAATTTACTCCAGAAAGAAATCCCATTACTCCAGTT AAGCTTGTTGACAGACAGCAAGCGGAACCATGGACACCCACAGCTAACCTGAAGATGCTCATTAGTGCTG CCAGCCCAGATATAAGGGACCGGGAGAAGAAAAAGGGACTATTCCGACCCATTGAAAACAAGGACGATGC ATTTACAGATTCTCTACAGCTTGATGTTGTTGGGGACAGTGCTGTGGACGAATTTGAAAAGCAAAGGCCA AGCAGAAAACAGAAAAGTTTAGGACTCCTGTGCCAGAAGTTTCTAGCTCGCTATCCAAGTTATCCCTTGT CAACTGAGAAAACTACCATCTCCCTAGATGAAGTTGCTGTCAGTCTTGGTGTGGAAAGGAGACGCATCTA TGACATTGTAAATGTGCTGGAGTCGCTGCATCTGGTCAGCCGGGTGGCTAAGAATCAGTATGGCTGGCAT GGACGGCACAGCCTGCCAAAAACCCTGAGGAACCTCCAGAGACTAGGAGAGGAGCAGAAATATGAAGAGC AAATGGCCTACCTCCAACAGAAAGAGCTGGACCTGATAGATTATAAATTTGGAGAACGTAAAAAAGATGG TGATCCAGATTCCCAGGAACAACAGTTACTGGATTTCTCTGAACCCGACTGTCCCTCTTCATCTGCAAAC AGTAGAAAAGACAAGTCTCTGAGAATTATGAGCCAGAAGTTTGTCATGCTGTTCCTCGTCTCCAAAACCA AGATTGTCACTCTGGATGTGGCTGCCAAAATACTGATAGAAGAAAGCCAAGATGCCCCGGACCATAGTAA ATTTAAAACAAAGGTACGACGCCTCTATGACATAGCCAATGTTCTGACCAGCTTGGCTCTGATAAAGAAA GTGCATGTAACAGAAGAGCGAGGTCGTAAACCAGCCTTCAAGTGGATCGGGCCTGTGGACTTCAGCTCAA GTGATGAAGAACTGGTGGATGTTTCTGCATCTGTCTTACCAGAATTGAAAAGAGAAACATATGGCCAGAT TCAAGTCTGTGCAAAACAGAAGCTGGCTCGCCATGGTTCTTTTAACACAGTTCAGGCTTCTGAGAGGATC CAGAGGAAAGTGAACTCAGAACCGAGCAGCCCGTACAGAGAAGAACAAGGATCAGGTGGCTACTCTTTAG AAATTGGAAGCCTGGCAGCTGTCTATAGACAGAAAATAGAAGACAATTCACAGGGAAAAGCCTTTGCCAG TAAGAGAGTGGTGCCTCCATCAAGCAGCTTGGACCCTGTTGCTCCTTTCCCTGTCCTCTCTGTTGACCCA GAATATTGTGTTAATCCTTTAGCCCACCCAGTATTTTCTGTTGCTCAGACGGACCTGCAGGCATTCTCCA TGCAGAACGGTCTGAATGGACAAGTGGATGTCTCACTTGCTTCTGCAGCCTCTGCTGTGGAGAGCCTGAA GCCAGCACTCCTTGCTGGCCAGCCTCTAGTGTATGTGCCCTCTGCCTCACTGTTCATGCTGTATGGAAGT CTGCAGGAGGGACCAGCGTCAGGGTCAGGGTCAGAGAGGGATGACAGAAGCTCAGAAGCCCCAGCCACAG TAGAGCTGTCATCTGCACCCTCAGCTCAGAAGCGCCTCTGTGAGGAGAGGAAACCTCAGGAGGAGGATGA GCCAGCCACTAAAAGGCAAAGTAGGGAATATGAAGACGGCCCGCTGTCGCTTGTCATGCCCAAGAAACCC TCAGATTCCACAGACCTTGCCTCTCCCAAGACTATGGGTAACAGGGCATCTATACCCCTCAAAGACATTC ATGTGAATGGCCAACTCCCTGCTGCAGAAGAGATTTCAGGAAAGGCAACAGCAAACTCTCTTGTTTCTTC TGAGTGGGGAAATCCTTCAAGAAATACAGATGTTGAAAAGCCTTCAAAAGAAAATGAAAGCACCAAAGAG CCTTCTTTGCTACAATATCTTTGTGTGCAGTCTCCTGCAGGATTAAATGGTTTCAATGTACTTTTATCTG GCAGTCAAACCCCCCCTACTGTGGGCCCGTCCTCAGGTCAGCTGCCGTCTTTCAGTGTCCCTTGCATGGT CTTACCATCTCCACCTCTGGGCCCTTTTCCTGTTCTCTATTCTCCTGCAATGCCGGGCCCGGTTTCTTCT ACTCTTGGTGCTCTCCCAAACACAGGACCTGTGAATTTCAGCTTGCCTGGCCTTGGATCAATAGCCCAGC TTCTCGTCGGCCCCACAGCTGTGGTTAATCCAAAGTCGTCCACACTCCCTTCTGCAGACCCTCAGCTTCA GAGTCAGCCCTCACTAAACCTAAGTCCAGTGATGTCAAGGTCACACAGTGTCGTCCAACAACCTGAGTCC CCCGTTTACGTGGGACATCCAGTCTCAGTAGTAAAATTACATCAGTCACCAGTTCCAGTGACCCCCAAGA GCATCCAACGCACACATCGTGAGACGTTTTTCAAGACACCCGGCAGCCTTGGAGACCCTGTCCTGAAGAG AAGAGAAAGGAACCAGTCACGAAACACCAGCTCGGCCCAGAGGAGACTAGAAATCCCCAGCGGCGGCGCT GAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217596 representing NM_203394
Red=Cloning site Green=Tags(s) MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKFTPERNPITPV KLVDRQQAEPWTPTANLKMLISAASPDIRDREKKKGLFRPIENKDDAFTDSLQLDVVGDSAVDEFEKQRP SRKQKSLGLLCQKFLARYPSYPLSTEKTTISLDEVAVSLGVERRRIYDIVNVLESLHLVSRVAKNQYGWH GRHSLPKTLRNLQRLGEEQKYEEQMAYLQQKELDLIDYKFGERKKDGDPDSQEQQLLDFSEPDCPSSSAN SRKDKSLRIMSQKFVMLFLVSKTKIVTLDVAAKILIEESQDAPDHSKFKTKVRRLYDIANVLTSLALIKK VHVTEERGRKPAFKWIGPVDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERI QRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDP EYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGS LQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKP SDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKE PSLLQYLCVQSPAGLNGFNVLLSGSQTPPTVGPSSGQLPSFSVPCMVLPSPPLGPFPVLYSPAMPGPVSS TLGALPNTGPVNFSLPGLGSIAQLLVGPTAVVNPKSSTLPSADPQLQSQPSLNLSPVMSRSHSVVQQPES PVYVGHPVSVVKLHQSPVPVTPKSIQRTHRETFFKTPGSLGDPVLKRRERNQSRNTSSAQRRLEIPSGGA D TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203394 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_203394.1, NP_976328.1 |
| RefSeq Size | 5728 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 144455 |
| UniProt ID | Q96AV8 |
| Protein Families | Transcription Factors |
| MW | 99.7 kDa |
| Gene Summary | E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression (Di Stefano et al., 2003 [PubMed 14633988]).[supplied by OMIM, May 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217596L3 | Lenti-ORF clone of E2F7 (Myc-DDK-tagged)-Human E2F transcription factor 7 (E2F7) |
CNY 5,890.00 |
|
| RC217596L4 | Lenti-ORF clone of E2F7 (mGFP-tagged)-Human E2F transcription factor 7 (E2F7) |
CNY 5,890.00 |
|
| RG217596 | E2F7 (tGFP-tagged) - Human E2F transcription factor 7 (E2F7) |
CNY 4,370.00 |
|
| SC308153 | E2F7 (untagged)-Human E2F transcription factor 7 (E2F7) |
CNY 6,680.00 |
