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TdT (DNTT) (NM_004088) Human Tagged ORF Clone
CAT＃: RC217586
DNTT (Myc-DDK-tagged)-Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1
ORF Plasmid: tGFP
"NM_004088" in other vectors (6)
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CNY 3,792.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TDT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217586 representing NM_004088
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCACCACGAGCGTCCCACTTGAGCCCTCGGAAGAAGAGACCCCGGCAGACGGGTGCCTTGATGG CCTCCTCTCCTCAAGACATCAAATTTCAAGATTTGGTCGTCTTCATTTTGGAGAAGAAAATGGGAACCAC CCGCAGAGCGTTCCTCATGGAGCTGGCCCGCAGGAAAGGGTTCAGGGTTGAAAATGAGCTCAGTGATTCT GTCACCCACATTGTAGCAGAGAACAACTCGGGTTCGGATGTTCTGGAGTGGCTTCAAGCACAGAAAGTAC AAGTCAGCTCACAACCAGAGCTCCTCGATGTCTCCTGGCTGATCGAATGCATAGGAGCAGGGAAACCGGT GGAAATGACAGGAAAACACCAGCTTGTTGTGAGAAGAGACTATTCAGATAGCACCAACCCAGGCCCCCCG AAGACTCCACCAATTGCTGTACAAAAGATCTCCCAGTATGCGTGTCAGAGAAGAACCACTTTAAACAACT GTAACCAGATATTCACGGATGCCTTTGATATACTGGCTGAAAACTGTGAGTTTAGAGAAAATGAAGACTC CTGTGTGACATTTATGAGAGCAGCTTCTGTATTGAAATCTCTGCCATTCACAATCATCAGTATGAAGGAC ACAGAAGGAATTCCCTGCCTGGGGTCCAAGGTGAAGGGTATCATAGAGGAGATTATTGAAGATGGAGAAA GTTCTGAAGTTAAAGCTGTGTTAAATGATGAACGATATCAATCCTTCAAACTCTTTACTTCTGTATTTGG AGTGGGGCTGAAGACTTCTGAGAAGTGGTTCAGGATGGGTTTCAGAACTCTGAGTAAAGTAAGGTCGGAC AAAAGCCTGAAATTTACACGAATGCAGAAAGCAGGATTTCTGTATTATGAAGACCTTGTCAGCTGTGTGA CCAGGGCAGAAGCAGAGGCCGTCAGTGTGCTGGTTAAAGAGGCTGTCTGGGCATTTCTTCCGGATGCTTT CGTCACCATGACAGGAGGGTTCCGGAGGGGTAAGAAGATGGGGCATGATGTAGATTTTTTAATTACCAGC CCAGGATCAACAGAGGATGAAGAGCAACTTTTACAGAAAGTGATGAACTTATGGGAAAAGAAGGGATTAC TTTTATATTATGACCTTGTGGAGTCAACATTTGAAAAGCTCAGGTTGCCTAGCAGGAAGGTTGATGCTTT GGATCATTTTCAAAAGTGCTTTCTGATTTTCAAATTGCCTCGTCAAAGAGTGGACAGTGACCAGTCCAGC TGGCAGGAAGGAAAGACCTGGAAGGCCATCCGTGTGGATTTAGTTCTGTGCCCCTACGAGCGTCGTGCCT TTGCCCTGTTGGGATGGACTGGCTCCCGGCAGTTTGAGAGAGACCTCCGGCGCTATGCCACACATGAGCG GAAGATGATTCTGGATAACCATGCTTTATATGACAAGACCAAGAGGATATTCCTCAAAGCAGAAAGTGAA GAAGAAATTTTTGCGCATCTGGGATTGGATTATATTGAACCGTGGGAAAGAAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217586 representing NM_004088
Red=Cloning site Green=Tags(s) MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDS VTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIGAGKPVEMTGKHQLVVRRDYSDSTNPGPP KTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKD TEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSD KSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITS PGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSS WQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESE EEIFAHLGLDYIEPWERNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004088 |
| ORF Size | 1527 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004088.2 |
| RefSeq Size | 2071 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 1791 |
| UniProt ID | P04053 |
| Protein Pathways | Hematopoietic cell lineage, Non-homologous end-joining |
| MW | 58.4 kDa |
| Gene Summary | This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217586L1 | Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, Myc-DDK-tagged |
CNY 6,192.00 |
|
| RC217586L2 | Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217586L3 | Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, Myc-DDK-tagged |
CNY 6,192.00 |
|
| RC217586L4 | Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, mGFP tagged |
CNY 6,192.00 |
|
| RG217586 | DNTT (tGFP-tagged) - Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1 |
CNY 5,392.00 |
|
| SC122668 | DNTT (untagged)-Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1 |
CNY 3,800.00 |
