IGF2BP2 (NM_001007225) Human Tagged ORF Clone

CAT#: RC217310

IGF2BP2 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001007225" in other vectors (4)

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CNY 4,144.00

CNY 4,940.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-IGF2BP2 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms IMP-2; IMP2; VICKZ2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217310 representing NM_001007225
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAACAAGCTTTACATCGGGAACCTGAGCCCCGCCGTCACCGCCGACGACCTCCGGCAGCTCTTTG
GGGACAGGAAGCTGCCCCTGGCGGGACAGGTCCTGCTGAAGTCCGGCTACGCCTTCGTGGACTACCCCGA
CCAGAACTGGGCCATCCGCGCCATCGAGACCCTCTCGGGTAAAGTGGAATTGCATGGGAAAATCATGGAA
GTTGATTACTCAGTCTCTAAAAAGCTAAGGAGCAGGAAAATTCAGATTCGAAACATCCCTCCTCACCTGC
AGTGGGAGGTGTTGGATGGACTTTTGGCTCAATATGGGACAGTGGAGAATGTGGAACAAGTCAACACAGA
CACAGAAACCGCCGTTGTCAACGTCACATATGCAACAAGAGAAGAAGCAAAAATAGCCATGGAGAAGCTA
AGCGGGCATCAGTTTGAGAACTACTCCTTCAAGATTTCCTACATCCCGGATGAAGAGGTGAGCTCCCCTT
CGCCCCCTCAGCGAGCCCAGCGTGGGGACCACTCTTCCCGGGAGCAAGGCCACGCCCCTGGGGGCACTTC
TCAGGCCAGACAGATTGATTTCCCGCTGCGGATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGA
AAGGAGGGCTTGACCATAAAGAACATCACTAAGCAGACCCAGTCCCGGGTAGATATCCATAGAAAAGAGA
ACTCTGGAGCTGCAGAGAAGCCTGTCACCATCCATGCCACCCCAGAGGGGACTTCTGAAGCATGCCGCAT
GATTCTTGAAATCATGCAGAAAGAGGCAGATGAGACCAAACTAGCCGAAGAGATTCCTCTGAAAATCTTG
GCACACAATGGCTTGGTTGGAAGACTGATTGGAAAAGAAGGCAGAAATTTGAAGAAAATTGAACATGAAA
CAGGGACCAAGATAACAATCTCATCTTTGCAGGATTTGAGCATATACAACCCGGAAAGAACCATCACTGT
GAAGGGCACAGTTGAGGCCTGTGCCAGTGCTGAGATAGAGATTATGAAGAAGCTGCGTGAGGCCTTTGAA
AATGATATGCTGGCTGTTAACACCCACTCCGGATACTTCTCCAGCCTGTACCCCCATCACCAGTTTGGCC
CGTTCCCGCATCATCACTCTTATCCAGAGCAGGAGATTGTGAATCTCTTCATCCCAACCCAGGCTGTGGG
CGCCATCATCGGGAAGAAGGGGGCACACATCAAACAGCTGGCGAGATTCGCCGGAGCCTCTATCAAGATT
GCCCCTGCGGAAGGCCCAGACGTCAGCGAAAGGATGGTCATCATCACCGGGCCACCGGAAGCCCAGTTCA
AGGCCCAGGGACGGATCTTTGGGAAACTGAAAGAGGAAAACTTCTTTAACCCCAAAGAAGAAGTGAAGCT
GGAAGCGCATATCAGAGTGCCCTCTTCCACAGCTGGCCGGGTGATTGGCAAAGGTGGCAAGACCGTGAAC
GAACTGCAGAACTTAACCAGTGCAGAAGTCATCGTGCCTCGTGACCAAACGCCAGATGAAAATGAGGAAG
TGATCGTCAGAATTATCGGGCACTTCTTTGCTAGCCAGACTGCACAGCGCAAGATCAGGGAAATTGTACA
ACAGGTGAAGCAGCAGGAGCAGAAATACCCTCAGGGAGTCGCCTCACAGCGCAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217310 representing NM_001007225
Red=Cloning site Green=Tags(s)

MMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIME
VDYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKL
SGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIG
KEGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKIL
AHNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFE
NDMLAVNTHSGYFSSLYPHHQFGPFPHHHSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKI
APAEGPDVSERMVIITGPPEAQFKAQGRIFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVN
ELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001007225
ORF Size 1668 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001007225.3
RefSeq Size 3547 bp
RefSeq ORF 1671 bp
Locus ID 10644
UniProt ID Q9Y6M1
MW 61.7 kDa
Gene Summary This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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