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DNA helicase HEL308 (HELQ) (NM_133636) Human Tagged ORF Clone
CAT＃: RC217270
- TrueORF®
HELQ (Myc-DDK-tagged)-Human helicase, POLQ-like (HELQ)
ORF Plasmid: tGFP
"NM_133636" in other vectors (4)
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CNY 11,496.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL308 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217270 representing NM_133636
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAATGTGGTTCCCGCATCCGCCGGCGGGTGTCTCTCCCCAAAAGGAACCGTCCAAGCTTGGGGT GTATTTTTGGCGCTCCCACCGCGGCCGAGCTCGAGCCCGGAGATGAGGGGAAAGAGGAGGAGGAAATGGT GGCTGAGAACAGGAGGCGGAAAACCGCGGGCGTACTGCCGGTTGAGGTACAGCCCCTTCTACTCTCAGAT TCCCCGGAATGTCTCGTCCTTGGAGGTGGTGATACAAACCCGGACCTCCTACGTCACATGCCCACTGACA GAGGGGTGGGAGACCAGCCTAATGACAGTGAAGTGGACATGTTTGGTGACTATGATAGCTTTACTGAAAA CTCCTTTATAGCTCAAGTTGACGACCTGGAACAAAAATATATGCAACTCCCTGAACATAAGAAACATGCT ACAGACTTTGCCACTGAAAATCTTTGCTCGGAAAGTATCAAAAACAAACTCAGCATTACTACCATAGGCA ACCTTACTGAATTACAAACTGATAAGCACACAGAGAACCAGAGTGGATATGAAGGTGTCACTATTGAACC TGGAGCTGATCTTTTGTATGATGTACCTTCCTCACAGGCTATATACTTTGAAAATTTGCAGAACTCTTCA AATGATTTGGGTGATCATTCTATGAAAGAAAGGGATTGGAAGTCATCCTCTCACAACACTGTGAATGAGG AACTGCCCCATAATTGCATAGAGCAACCCCAGCAAAATGATGAGTCCTCTTCCAAAGTCAGAACTAGTTC AGATATGAACAGGAGAAAAAGTATTAAAGATCATCTAAAAAATGCCATGACTGGAAATGCGAAGGCCCAG ACACCAATATTTTCTAGAAGTAAACAGCTCAAAGACACTCTCCTATCTGAGGAAATTAATGTTGCTAAGA AAACAGTTGAGTCATCATCAAATGACCTTGGTCCTTTTTATTCATTACCCAGCAAAGTGAGAGACCTTTA TGCCCAATTCAAGGGAATTGAAAAATTATATGAATGGCAACATACTTGTTTAACATTGAATTCTGTGCAA GAAAGAAAAAATTTAATATATTCCTTGCCAACAAGTGGTGGAAAAACCCTCGTGGCTGAGATTTTAATGC TGCAAGAACTGCTTTGCTGTCGGAAAGATGTTTTAATGATTCTTCCATATGTGGCAATTGTCCAAGAAAA GATTTCAGGTTTGTCAAGTTTTGGTATAGAACTCGGTTTCTTTGTTGAAGAATATGCTGGAAGCAAAGGA AGATTTCCTCCAACTAAAAGAAGGGAAAAGAAATCACTCTATATTGCCACTATTGAAAAAGGACATAGCT TGGTGAACTCCTTGATTGAAACTGGAAGAATTGACAGTCTGGGTCTGGTTGTTGTAGACGAGTTGCACAT GATTGGTGAAGGAAGCCGTGGAGCTACACTGGAAATGACCCTAGCAAAAATCCTCTACACTAGCAAAACG ACTCAAATTATTGGTATGAGTGCAACATTAAACAATGTTGAAGACCTACAAAAGTTTCTTCAAGCAGAAT ATTATACCAGTCAATTTAGACCAGTTGAGTTAAAAGAATATCTGAAAATAAATGATACAATATATGAAGT TGACAGCAAAGCTGAGAATGGCATGACTTTTTCACGTCTTCTTAATTATAAGTATTCTGATACCCTAAAA AAGATGGATCCTGATCACTTGGTAGCATTGGTGACAGAAGTTATTCCCAATTATTCCTGCTTAGTTTTTT GTCCTAGTAAGAAGAACTGTGAAAATGTAGCAGAAATGATATGCAAATTTTTAAGCAAGGAATATCTGAA ACATAAGGAGAAAGAAAAATGTGAGGTGATTAAGAACTTGAAGAATATTGGCAATGGCAACCTGTGTCCT GTTTTAAAGCGCACTATCCCATTTGGAGTTGCCTATCACCACAGTGGCTTAACAAGTGATGAAAGGAAAC TCTTGGAGGAGGCCTACTCCACAGGAGTGCTCTGTCTTTTTACCTGCACATCTACCCTAGCGGCAGGTGT CAACCTACCAGCTCGAAGAGTTATTTTAAGAGCTCCCTATGTTGCTAAGGAATTTTTAAAGAGGAATCAA TATAAACAGATGATTGGCAGAGCTGGTCGTGCTGGAATAGATACTATTGGGGAGAGTATCCTCATATTGC AAGAAAAAGACAAACAACAGGTATTGGAGTTAATAACTAAACCATTGGAAAACTGTTACAGCCATCTTGT TCAGGAATTCACCAAGGGAATCCAAACATTATTTCTCTCTTTGATTGGTTTGAAGATTGCAACGAATCTT GATGACATCTATCATTTCATGAATGGTACATTTTTTGGTGTTCAGCAAAAGGTTTTATTGAAAGAAAAAA GTCTCTGGGAAATAACTGTTGAATCACTTAGATACCTGACAGAAAAAGGACTCCTACAAAAAGACACTAT TTATAAGTCTGAAGAAGAGGTCCAATATAATTTTCATATTACAAAGTTGGGACGTGCTTCATTTAAGGGA ACTATAGATTTAGCTTATTGTGACATTCTGTACAGAGACTTGAAGAAAGGTCTTGAAGGACTTGTGCTTG AAAGCCTTCTTCATCTAATCTACCTAACAACCCCCTATGATCTGGTTTCACAGTGTAACCCTGATTGGAT GATATACTTCAGGCAGTTTAGCCAACTCAGTCCAGCAGAACAAAATGTAGCTGCCATTCTTGGAGTCTCT GAAAGCTTTATTGGGAAGAAAGCATCAGGCCAAGCCATCGGAAAGAAGGTGGACAAGAACGTTGTCAACA GGCTATATCTGTCTTTTGTTCTTTATACCTTGCTCAAAGAGACCAACATTTGGACTGTATCTGAAAAATT TAATATGCCTCGAGGATATATACAAAATCTTCTCACTGGAACTGCCTCATTCTCATCTTGTGTGTTACAT TTCTGTGAGGAGCTTGAGGAGTTTTGGGTTTACAGAGCCCTTTTGGTAGAACTTACCAAGAAGCTGACTT ACTGTGTAAAGGCAGAATTAATCCCTCTCATGGAAGTTACTGGAGTTTTAGAGGGTCGAGCAAAACAGTT ATACAGTGCAGGTTACAAAAGTCTAATGCACTTAGCTAATGCAAATCCTGAAGTGCTCGTAAGGACAATT GATCATTTATCAAGACGCCAAGCCAAGCAAATTGTTTCATCAGCAAAGATGCTGTTGCATGAAAAAGCAG AAGCCCTGCAAGAAGAGGTAGAAGAGTTACTAAGATTGCCTTCTGATTTCCCTGGTGCTGTGGCTTCTTC CACTGACAAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217270 representing NM_133636
Red=Cloning site Green=Tags(s) MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELEPGDEGKEEEEMVAENRRRKTAGVLPVEVQPLLLSD SPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFIAQVDDLEQKYMQLPEHKKHA TDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGYEGVTIEPGADLLYDVPSSQAIYFENLQNSS NDLGDHSMKERDWKSSSHNTVNEELPHNCIEQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQ TPIFSRSKQLKDTLLSEEINVAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQ ERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKG RFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKT TQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLK KMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCP VLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQ YKQMIGRAGRAGIDTIGESILILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNL DDIYHFMNGTFFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKG TIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAEQNVAAILGVS ESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMPRGYIQNLLTGTASFSSCVLH FCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVLEGRAKQLYSAGYKSLMHLANANPEVLVRTI DHLSRRQAKQIVSSAKMLLHEKAEALQEEVEELLRLPSDFPGAVASSTDKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133636 |
| ORF Size | 3303 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133636.4 |
| RefSeq Size | 3596 bp |
| RefSeq ORF | 3306 bp |
| Locus ID | 113510 |
| UniProt ID | Q8TDG4 |
| Domains | DEAD, helicase_C |
| MW | 124.6 kDa |
| Gene Summary | HEL308 is a single-stranded DNA-dependent ATPase and DNA helicase (Marini and Wood, 2002 [PubMed 11751861]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217270L3 | Lenti-ORF clone of HELQ (Myc-DDK-tagged)-Human helicase, POLQ-like (HELQ) |
CNY 12,920.00 |
|
| RC217270L4 | Lenti-ORF clone of HELQ (mGFP-tagged)-Human helicase, POLQ-like (HELQ) |
CNY 12,920.00 |
|
| RG217270 | HELQ (tGFP-tagged) - Human helicase, POLQ-like (HELQ) |
CNY 12,160.00 |
|
| SC120510 | HELQ (untagged)-Human helicase, POLQ-like (HELQ) |
CNY 21,280.00 |
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