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ANKLE2 (NM_015114) Human Tagged ORF Clone
CAT＃: RC216867
ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2)
ORF Plasmid: tGFP
"NM_015114" in other vectors (6)
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CNY 6,872.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIAA0692; Lem4; LEMD7; MCPH16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216867 representing NM_015114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGCCGCGGCTGGCGGCGGCCGAGTGGGCGGCGCTGGCCTGGGAGCTGCTGGGCGCCTCGGTGC TGCTGATCGCTGTGCGGTGGCTGGTGCGGCGGCTGGGCCCGCGGCCGGGAGGTCTGGGCCGCAGCGGGAC CCCAGTTCCTCCGCCAAGCGCGGCCGCCGCCCCCGCCTCAGGTGAAATGACAATGGATGCTCTGTTGGCT CGATTGAAACTTCTGAATCCAGATGACCTTAGAGAAGAAATCGTCAAAGCCGGATTGAAATGTGGACCCA TTACATCAACTACAAGGTTCATTTTTGAGAAAAAATTGGCTCAGGCTTTACTGGAGCAAGGAGGAAGGCT GTCTTCTTTCTACCACCATGAGGCAGGTGTCACAGCTCTCAGCCAGGACCCACAAAGGATTTTGAAGCCA GCTGAAGGGAACCCAACTGATCAGGCTGGTTTTTCTGAAGACAGAGATTTTGGTTACAGTGTGGGCCTGA ATCCTCCAGAGGAGGAAGCTGTGACATCCAAGACCTGCTCGGTGCCCCCTAGTGACACCGACACCTACAG AGCTGGAGCGACTGCGTCTAAGGAGCCGCCCCTGTACTATGGGGTGTGTCCAGTGTATGAGGACGTCCCA GCGAGAAATGAAAGGATCTATGTTTATGAAAATAAAAAGGAAGCATTGCAAGCTGTCAAGATGATCAAAG GGTCCCGATTTAAAGCTTTTTCTACCAGAGAAGACGCTGAGAAATTTGCTAGAGGAATTTGTGATTATTT CCCTTCTCCAAGCAAAACGTCCTTACCACTGTCTCCTGTGAAAACAGCTCCACTCTTTAGCAATGACAGG TTGAAAGATGGTTTGTGCTTGTCGGAATCAGAAACAGTCAACAAAGAGCGAGCGAACAGTTACAAAAATC CCCGCACGCAGGACCTCACCGCCAAGCTTCGGAAAGCTGTGGAGAAGGGAGAGGAGGACACCTTTTCTGA CCTTATCTGGAGCAACCCCCGGTATCTGATAGGCTCAGGAGACAACCCCACTATCGTGCAGGAAGGGTGC AGGTACAACGTGATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCATCTGCCAGCTGACTCTGGACGTCC TGGAGAACCCTGACTTCATGAGGCTGATGTACCCTGATGACGACGAGGCCATGCTGCAGAAGCGTATCCG TTACGTGGTGGACCTGTACCTCAACACCCCCGACAAGATGGGCTATGACACACCGTTGCATTTTGCTTGT AAGTTTGGAAATGCAGATGTAGTCAACGTGCTTTCGTCACACCATTTGATTGTAAAAAACTCAAGGAATA AATATGATAAAACACCTGAAGATGTAATTTGTGAAAGAAGCAAAAATAAATCTGTGGAACTGAAGGAGCG GATCAGAGAGTATTTAAAGGGCCACTACTACGTGCCCCTCCTGAGAGCGGAAGAGACTTCTTCTCCAGTC ATCGGGGAGCTGTGGTCCCCAGACCAGACGGCTGAGGCCTCTCACGTCAGCCGCTATGGAGGCAGCCCCA GAGACCCGGTACTGACCCTGAGAGCCTTCGCAGGGCCCCTGAGTCCAGCCAAGGCAGAAGATTTTCGCAA GCTCTGGAAAACTCCACCTCGAGAGAAAGCAGGCTTCCTTCACCACGTCAAGAAGTCGGACCCGGAAAGA GGCTTTGAGAGAGTGGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCTGGGTTGAATACTGGGAATTTC TGGGCTGTTTTGTTGATCTGTCTTCCCAGGAAGGCCTGCAAAGACTAGAAGAATATCTCACACAGCAGGA AATAGGCAAAAAGGCTCAACAAGAAACAGGAGAACGGGAAGCCTCCTGCCGAGATAAAGCCACCACGTCT GGCAGCAATTCCATTTCCGTGAGGGCGTTTCTAGATGAAGATGACATGAGCTTGGAAGAAATAAAAAATC GGCAAAATGCAGCTCGAAATAACAGCCCGCCCACAGTCGGTGCTTTTGGACATACGAGGTGCAGCGCCTT CCCCTTGGAGCAGGAGGCAGACCTCATAGAAGCCGCCGAGCCGGGAGGTCCACACAGCAGCAGAAATGGG CTCTGCCATCCTCTGAATCACAGCAGGACCCTGGCGGGCAAGAGACCAAAGGCCCCCCGTGGGGAGGAAG CCCATCTGCCACCTGTCTCGGATTTGACTGTTGAGTTTGATAAACTGAATTTGCAAAATATAGGACGTAG CGTTTCCAAGACACCAGATGAAAGTACAAAAACTAAAGATCAGATCCTGACTTCAAGAATCAATGCAGTA GAAAGAGACTTGTTAGAGCCTTCTCCCGCAGACCAACTCGGGAATGGCCACAGGAGGACAGAAAGTGAAA TGTCAGCCAGGATCGCTAAAATGTCCTTGAGTCCCAGCAGCCCCAGGCACGAGGATCAGCTCGAGGTCAC CAGGGAACCGGCCAGGCGGCTCTTCCTTTTTGGAGAGGAGCCATCAAAACTCGATCAGGATGTTTTGGCC GCTCTTGAATGTGCAGACGTCGACCCCCATCAGTTCCCGGCCGTGCACAGATGGAAGAGTGCTGTCCTGT GCTACTCACCCTCGGACAGACAGAGTTGGCCCAGTCCCGCGGTGAAAGGAAGGTTCAAGTCTCAGCTGCC AGATCTCAGTGGCCCTCACAGCTACAGTCCGGGGAGAAACAGCGTGGCTGGAAGCAACCCCGCAAAGCCA GGCCTGGGCAGTCCTGGGCGCTACAGCCCCGTGCACGGGAGCCAGCTCCGCAGGATGGCGCGCCTGGCTG AGCTTGCCGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216867 representing NM_015114
Red=Cloning site Green=Tags(s) MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLA RLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKP AEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVP ARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDR LKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGC RYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFAC KFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPV IGELWSPDQTAEASHVSRYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPER GFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTS GSNSISVRAFLDEDDMSLEEIKNRQNAARNNSPPTVGAFGHTRCSAFPLEQEADLIEAAEPGGPHSSRNG LCHPLNHSRTLAGKRPKAPRGEEAHLPPVSDLTVEFDKLNLQNIGRSVSKTPDESTKTKDQILTSRINAV ERDLLEPSPADQLGNGHRRTESEMSARIAKMSLSPSSPRHEDQLEVTREPARRLFLFGEEPSKLDQDVLA ALECADVDPHQFPAVHRWKSAVLCYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKP GLGSPGRYSPVHGSQLRRMARLAELAAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015114 |
| ORF Size | 2814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015114.3 |
| RefSeq Size | 4491 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 23141 |
| UniProt ID | Q86XL3 |
| Protein Families | Transmembrane |
| MW | 104.1 kDa |
| Gene Summary | This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
LEM4 confers tamoxifen resistance to breast cancer cells by activating cyclin D-CDK4/6-Rb and ERα pathway
,Gao, A;Sun, T;Ma, G;Cao, J;Hu, Q;Chen, L;Wang, Y;Wang, Q;Sun, J;Wu, R;Wu, Q;Zhou, J;Liu, L;Hu, J;Dong, JT;Zhu, Z;,
Nat Commun
,PubMed ID 30301939
[ANKLE2]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216867L1 | Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 9,272.00 |
|
| RC216867L2 | Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 5,890.00 |
|
| RC216867L3 | Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 5,890.00 |
|
| RC216867L4 | Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 5,890.00 |
|
| RG216867 | ANKLE2 (tGFP-tagged) - Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 8,472.00 |
|
| SC316434 | ANKLE2 (untagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) |
CNY 7,220.00 |
