CENTD1 (ARAP2) (NM_015230) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CENTD1; PARX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216798 representing NM_015230 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCAGTCAGTGAAGTAAATGTGGATATAAAAGATTTCCTAATGAGCATTAATTTGGAGCAGTATC TCTTACATTTCCATGAGTCTGGTTTTACTACTGTGAAGGACTGTGCAGCAATAAATGACAGCCTGCTGCA GAAAATTGGAATATCACCTACAGGTCACCGTAGGAGGATACTTAAACAGTTACAGATAATCTTGTCAAAA ATGCAAGATATTCCAATATATGCAAATGTTCATAAAACTAAGAAGAATGATGACCCTTCAAAGGATTACC ATGTTCCATCTTCTGATCAGAATATCTGCATAGAACTTTCCAATTCTGGTAGTGTTCAGACATCTAGCCC ACCGCAGTTGGAGACTGTTAGAAAAAATCTTGAAGACAGTGATGCAAGTGTGGAAAGAAGCCAGTATCCT CAATCAGATGATAAGCTGTCTCCTCCTAAACGCGACTTCCCCACTGCAGAGGAACCACACCTGAATTTGG GTTCTTTGAATGATTCTTTATTTGGTAGTGACAATATTAAAATAGAATCATTGATTACAAAGAAGACTGT GGATCACACAGTTGAAGAACAACAAACAGAAAAAGTTAAATTGATCACAGAAAATCTCAGTAAGCTCCCT AATGCAGACTCTGAATGCCTTTCTTTTGTTGGCTGTTCAACATCAGGAACAAATTCTGGAAATGGAACAA ATGGTTTATTAGAAGGATCACCACCATCCCCATTCTTTAAGTTTCAAGGAGAAATGATTGTAAATGACTT GTATGTTCCATCATCACCAATCCTAGCACCTGTGAGAAGTCGTAGCAAGTTGGTTTCAAGACCATCTCGA TCTTTTCTGCTAAGACATCGACCTGTACCAGAGATTCCAGGGTCAACAAAAGGAGTTTCTGGGAGCTATT TCCGTGAAAGAAGAAATGTTGCTACCTCAACTGAAAAATCTGTGGCATGGCAAAATTCAAATGAGGAGAA TTCATCTTCCATCTTTCCTTATGGAGAGACCTTTCTCTTCCAGAGACTAGAAAATTCCAAGAAGCGATCT ATAAAGAATGAATTTTTGACCCAGGGAGAAGCACTCAAAGGGGAAGCAGCTACTGCAACAAACTCTTTTA TCATCAAATCAAGCATATACGATAACAGAAAGGAGAAAATAAGCGAGGACAAGGTGGAAGATATTTGGAT ACCTCGAGAGGACAAAAACAATTTTTTGATAGACACTGCTTCTGAATCAGAATACTCAACAGTAGAAGAA TGCTTTCAGAGTTTAAGAAGAAAAAATTCAAAGGCATCTAAATCTAGGACTCAAAAAGCCTTGATTTTGG ACTCCGTTAATAGGCACAGTTATCCGTTAAGCTCAACAAGTGGAAATGCTGATTCATCAGCCGTTTCTTC ACAGGCAATATCTCCCTATGCCTGCTTTTATGGAGCATCTGCAAAGAAGGTTAAATCAGGATGGCTGGAT AAACTCTCTCCTCAAGGAAAACGCATGTTTCAAAAGAGATGGGTGAAATTTGATGGCCTTAGCATTTCTT ACTACAATAATGAGAAGGAGATGTATTCGAAAGGAATAATTCCCCTTTCTGCTATATCAACAGTACGAGT TCAAGGAGACAACAAATTTGAAGTTGTTACAACACAAAGAACTTTTGTTTTTAGAGTAGAAAAAGAAGAG GAGAGAAATGACTGGATCAGCATACTATTAAATGCACTGAAATCACAATCCCTTACCTCGCAGTCTCAAG CTGTTGTTACACCTGAGAAATGTGGATATCTTGAATTGAGAGGCTATAAGGCAAAAATTTTTACTGTGTT AAGTGGAAACAGTGTGTGGCTTTGCAAAAACGAACAGGATTTTAAGAGTGGACTTGGTATTACCATAATT CCTATGAATGTAGCAAATGTAAAGCAAGTGGACCGAACTGTGAAACAATCTTTTGAAATAATCACTCCCT ACAGGAGTTTCAGCTTTACAGCCGAGACTGAAAAGGAGAAACAAGACTGGATTGAAGCTGTGCAGCAATC AATAGCAGAAACTCTCTCTGATTATGAAGTAGCTGAGAAGATTTGGTTCAATGAATCCAACAGGAGCTGT GCAGATTGTAAAGCCCCAGATCCTGACTGGGCATCCATCAATCTCTGTGTTGTCATCTGTAAGAAGTGTG CAGGACAGCATAGATCTTTAGGACCAAAAGATTCCAAGGTTAGAAGTCTAAAAATGGATGCTAGCATTTG GAGCAATGAACTCATCGAGCTTTTTATTGTCATTGGAAACAAAAGAGCAAATGACTTTTGGGCTGGTAAT CTTCAAAAGGATGAAGAATTACATATGGACTCACCAGTAGAAAAGAGAAAAAACTTTATTACTCAGAAAT ATAAAGAAGGAAAATTCAGAAAAACTCTTTTGGCATCTCTCACCAAAGAAGAATTAAATAAGGCTCTATG TGCTGCTGTAGTGAAACCGGATGTTCTAGAAACAATGGCTTTGCTGTTCAGTGGAGCAGATGTCATGTGT GCCACCGGAGACCCCGTGCATAGCACCCCCTATCTGCTAGCCAAGAAAGCTGGGCAAAGTCTGCAAATGG AATTTCTCTACCATAACAAATTCTCAGATTTCCCTCAACATGATATTCATTCCGAGGGTGTATTAAGTCA AGAGTCTTCCCAGTCCACATTCCTCTGTGACTTTTTATATCAAGCTCCTTCTGCTGCTTCTAAACTCTCT TCAGAGAAAAAACTGCTTGAAGAGACAAATAAAAAATGGTGTGTTTTGGAAGGAGGCTTCTTGAGTTACT ATGAAAATGATAAGTCTACCACACCTAATGACACCATTAATATCAATGAAGTTATCTGCCTGGCTATACA CAAAGAGGACTTCTATTTAAATACTGGGCCCATCTTTATCTTTGAGATCTACTTACCCTCCGAACGTGTG TTTTTATTTGGAGCTGAAACATCTCAAGCTCAAAGAAAATGGACAGAGGCAATAGCCAAGCATTTTGTTC CCTTATTTGCTGAAAACTTAACAGAAGCTGACTATGATTTGATTGGTCAACTCTTCTACAAAGACTGCCA TGCCCTGGATCAGTGGAGAAAAGGCTGGTTTGCTATGGACAAATCCAGCTTGCATTTTTGCCTTCAAATG CAAGAAGTTCAGGGAGATAGAATGCACTTAAGAAGACTGCAAGAGCTAACAATCAGCACAATGGTTCAAA ATGGGGAAAAACTGGATGTTTTACTCTTGGTAGAAAAAGGGAGAACATTATACATCCATGGGCATACCAA GTTGGATTTCACAGTCTGGCATACTGCAATTGAAAAAGCAGCAGGTACAGATGGTAATGCTTTACAAGAT CAGCAGCTCAGCAAAAATGACGTTCCCATTATAGTGAACAGCTGTATAGCATTTGTTACACAGTATGGTT TAGGATGCAAATATATCTATCAAAAGAATGGTGATCCTTTGCATATAAGTGAACTCCTGGAGAGTTTCAA AAAGGATGCAAGAAGCTTTAAATTGAGGGCTGGAAAACATCAGCTTGAAGATGTGACGGCTGTGTTGAAA AGTTTTCTCTCTGACATTGATGATGCACTGCTTACTAAGGAGCTCTACCCATATTGGATCTCTGCTTTAG ATACGCAAGATGACAAGGAAAGAATTAAAAAATATGGAGCATTTATACGTTCTCTTCCAGGGGTCAACCG AGCAACACTAGCAGCTATCATTGAACACCTGTATAGGGTTCAGAAATGCTCAGAAATCAATCACATGAAT GCCCATAATTTGGCCTTGGTCTTTTCATCCTGTTTGTTTCAAACGAAGGGACAAACTAGTGAAGAAGTGA ATGTAATTGAGGACCTAATTAATAATTATGTAGAAATATTTGAGGTTAAAGAAGATCAAGTCAAACAAAT GGACATAGAAAATAGCTTTATTACCAAGTGGAAAGACACCCAAGTTTCCCAGGCTGGAGATTTGTTAATT GAAGTATATGTAGAAAGGAAGGAACCCGACTGTAGTATTATAATTCGGATATCTCCTGTGATGGAAGCAG AAGAATTAACTAATGATATATTAGCGATAAAAAATATTATTCCTACAAAAGGTGATATTTGGGCCACATT TGAAGTCATTGAAAATGAAGAGCTAGAGCGTCCTCTTCACTACAAGGAAAATGTACTGGAGCAGGTGCTT CGGTGGAGTTCATTAGCTGAACCTGGCTCTGCTTACCTGGTGGTGAAGAGATTCTTAACCGCTGACACAA TTAAACACTGCAGTGACCGGAGTACACTGGGAAGCATCAAAGAAGGAATCTTGAAAATCAAAGAAGAACC ATCCAAAATACTATCTGGAAATAAGTTTCAAGACCGGTATTTTGTTTTACGAGATGGGTTTCTCTTTCTT TACAAGGATGTGAAGAGTAGTAAACATGACAAGATGTTTTCTCTCAGTTCCATGAAGTTTTATCGTGGAG TGAAAAAGAAAATGAAGCCTCCAACAAGCTGGGGATTGACCGCATATTCTGAGAAACATCACTGGCACCT GTGTTGTGATAGTTCACAAACTCAGACGGAGTGGATGACCAGTATCTTTATTGCCCAGCATGAATATGAT ATATGGCCACCAGCTGGAAAGGAACGAAAACGTTCAATAACCAAAAATCCCAAAATTGGAGGTTTGCCTC TGATTCCTATACAGCATGAGGGGAATGCAACCTTGGCCCGGAAAAATATTGAGAGTGCAAGAGCAGAACT TGAAAGGCTGCGGCTCAGTGAAAAGTGTGATAAAGAGTCCGTGGACTCTAGCTTAAAGGAGAGAGCTTCC ATGGTGGCCCACTGCCTGGAGCACAAGGACGATAAACTTCGAAATCGACCCCGAAAACATCGGAGTTTCA ACTGCCTGGAGGACACAGAGCCTGAAGCCCCACTTGGGCAACCAAAAGGCCATAAAGGCCTAAAGACATT GAGGAAGACTGAGGACAGGAATAGCAAAGCTACTTTGGACTCTGATCATAAGTTACCATCAAGGGTAATT GAAGAACTTAATGTGGTTCTACAACGGTCAAGAACCCTTCCAAAAGAATTACAGGATGAGCAGATTTTGA AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216798 representing NM_015230 Red=Cloning site Green=Tags(s) MSSVSEVNVDIKDFLMSINLEQYLLHFHESGFTTVKDCAAINDSLLQKIGISPTGHRRRILKQLQIILSK MQDIPIYANVHKTKKNDDPSKDYHVPSSDQNICIELSNSGSVQTSSPPQLETVRKNLEDSDASVERSQYP QSDDKLSPPKRDFPTAEEPHLNLGSLNDSLFGSDNIKIESLITKKTVDHTVEEQQTEKVKLITENLSKLP NADSECLSFVGCSTSGTNSGNGTNGLLEGSPPSPFFKFQGEMIVNDLYVPSSPILAPVRSRSKLVSRPSR SFLLRHRPVPEIPGSTKGVSGSYFRERRNVATSTEKSVAWQNSNEENSSSIFPYGETFLFQRLENSKKRS IKNEFLTQGEALKGEAATATNSFIIKSSIYDNRKEKISEDKVEDIWIPREDKNNFLIDTASESEYSTVEE CFQSLRRKNSKASKSRTQKALILDSVNRHSYPLSSTSGNADSSAVSSQAISPYACFYGASAKKVKSGWLD KLSPQGKRMFQKRWVKFDGLSISYYNNEKEMYSKGIIPLSAISTVRVQGDNKFEVVTTQRTFVFRVEKEE ERNDWISILLNALKSQSLTSQSQAVVTPEKCGYLELRGYKAKIFTVLSGNSVWLCKNEQDFKSGLGITII PMNVANVKQVDRTVKQSFEIITPYRSFSFTAETEKEKQDWIEAVQQSIAETLSDYEVAEKIWFNESNRSC ADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDSKVRSLKMDASIWSNELIELFIVIGNKRANDFWAGN LQKDEELHMDSPVEKRKNFITQKYKEGKFRKTLLASLTKEELNKALCAAVVKPDVLETMALLFSGADVMC ATGDPVHSTPYLLAKKAGQSLQMEFLYHNKFSDFPQHDIHSEGVLSQESSQSTFLCDFLYQAPSAASKLS SEKKLLEETNKKWCVLEGGFLSYYENDKSTTPNDTININEVICLAIHKEDFYLNTGPIFIFEIYLPSERV FLFGAETSQAQRKWTEAIAKHFVPLFAENLTEADYDLIGQLFYKDCHALDQWRKGWFAMDKSSLHFCLQM QEVQGDRMHLRRLQELTISTMVQNGEKLDVLLLVEKGRTLYIHGHTKLDFTVWHTAIEKAAGTDGNALQD QQLSKNDVPIIVNSCIAFVTQYGLGCKYIYQKNGDPLHISELLESFKKDARSFKLRAGKHQLEDVTAVLK SFLSDIDDALLTKELYPYWISALDTQDDKERIKKYGAFIRSLPGVNRATLAAIIEHLYRVQKCSEINHMN AHNLALVFSSCLFQTKGQTSEEVNVIEDLINNYVEIFEVKEDQVKQMDIENSFITKWKDTQVSQAGDLLI EVYVERKEPDCSIIIRISPVMEAEELTNDILAIKNIIPTKGDIWATFEVIENEELERPLHYKENVLEQVL RWSSLAEPGSAYLVVKRFLTADTIKHCSDRSTLGSIKEGILKIKEEPSKILSGNKFQDRYFVLRDGFLFL YKDVKSSKHDKMFSLSSMKFYRGVKKKMKPPTSWGLTAYSEKHHWHLCCDSSQTQTEWMTSIFIAQHEYD IWPPAGKERKRSITKNPKIGGLPLIPIQHEGNATLARKNIESARAELERLRLSEKCDKESVDSSLKERAS MVAHCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKATLDSDHKLPSRVI EELNVVLQRSRTLPKELQDEQILK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015230
ORF Size	5112 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015230.4
RefSeq Size	7360 bp
RefSeq ORF	5115 bp
Locus ID	116984
UniProt ID	Q8WZ64
Domains	RhoGAP, ArfGap, SAM, PH
Protein Pathways	Endocytosis
MW	193.3 kDa
Gene Summary	The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq, Sep 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.