MCM8 (NM_182802) Human Tagged ORF Clone
CAT#: RC216686
- TrueORF®
MCM8 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 8 (MCM8), transcript variant 2
ORF Plasmid: tGFP
"NM_182802" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C20orf154; dJ967N21.5; POF10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216686 representing NM_182802
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGAGTATAGAGGCAGAGGATTTGGACGAGGAAGATTTCAAAGCTGGAAAAGGGGAAGAGGTG GTGGGAACTTCTCAGGAAAATGGAGAGAAAGAGAACACAGACCTGATCTGAGTAAAACCACAGGAAAACG TACTTCTGAACAAACCCCACAGTTTTTGCTTTCAACAAAGACCCCACAGTCAATGCAGTCAACATTGGAT CGATTCATACCATATAAAGGCTGGAAGCTTTATTTCTCTGAAGTTTACAGCGATAGCTCTCCTTTGATTG AGAAGATTCAAGCATTTGAAAAATTTTTCACAAGGCATATTGATTTGTATGACAAGGATGAAATAGAAAG AAAGGGAAGTATTTTGGTAGATTTTAAAGAACTGACAGAAGGTGGTGAAGTAACTAACTTGATACCAGAT ATAGCAACTGAACTAAGAGATGCACCTGAGAAAACCTTGGCTTGCATGGGTTTGGCAATACATCAGGTGT TAACTAAGGACCTTGAAAGGCATGCAGCTGAGTTACAAGCCCAGGAAGGATTGTCTAATGATGGAGAAAC AATGGTAAATGTGCCACATATTCATGCAAGGGTGTACAACTATGAGCCTTTGACACAGCTCAAGAATGTC AGAGCAAATTACTATGGAAAATACATTGCTCTAAGAGGGACAGTGGTTCGTGTCAGTAATATAAAGCCTC TTTGCACCAAGATGGCTTTTCTTTGTGCTGCATGTGGAGAAATTCAGAGCTTTCCTCTTCCAGATGGAAA ATACAGTCTTCCCACAAAGTGTCCTGTGCCTGTGTGTCGAGGCAGGTCATTTACTGCTCTCCGCAGCTCT CCTCTCACAGTTACGATGGACTGGCAGTCAATCAAAATCCAGGAATTGATGTCTGATGATCAGAGAGAAG CAGGTCGGATTCCACGAACAATAGAATGTGAGCTTGTTCATGATCTTGTGGATAGCTGTGTCCCGGGAGA CACAGTGACTATTACTGGAATTGTCAAAGTCTCAAATGCGGAAGAAGCAAATTCTATTAGTAATAGCAAA GGACAGAAAACAAAGAGTTCTGAGGATGGGTGTAAGCATGGAATGTTGATGGAGTTCTCACTTAAAGACC TTTATGCCATCCAAGAGATTCAAGCTGAAGAAAACCTGTTTAAACTCATTGTCAACTCGCTTTGCCCTGT CATTTTTGGTCATGAACTTGTTAAAGCAGGTTTGGCATTAGCACTCTTTGGAGGAAGCCAGAAATACGCA GATGACAAAAACAGAATTCCAATTCGGGGAGACCCCCACATCCTTGTTGTTGGAGATCCAGGCCTAGGAA AAAGTCAAATGCTACAGGCAGCGTGCAATGTTGCCCCACGTGGCGTGTATGTTTGTGGTAACACCACGAC CACCTCTGGTCTGACGGTAACTCTTTCAAAAGATAGTTCCTCTGGAGATTTTGCTTTGGAAGCTGGTGCC CTGGTACTTGGTGATCAAGGTATTTGTGGAATCGATGAATTTGATAAGATGGGGAATCAACATCAAGCCT TGTTGGAAGCCATGGAGCAGCAAAGTATTAGTCTTGCTAAGGCTGGTGTGGTTTGTAGCCTTCCTGCAAG AACTTCCATTATTGCTGCTGCAAATCCAGTTGGAGGACATTACAATAAAGCCAAAACAGTTTCTGAGAAT TTAAAAATGGGGAGTGCACTACTATCCAGATTTGATTTGGTCTTTATCCTGTTAGATACTCCAAATGAGC ATCATGATCACTTACTCTCTGAACATGTGATTGCAATAAGAGCTGGAAAGCAGAGAACCATTAGCAGTGC CACAGTAGCTCGTATGAATAGTCAAGATTCAAATACTTCCGTACTTGAAGTAGTTTCTGAGAAGCCATTA TCAGAAAGACTAAAGGTGGTTCCTGGAGAAACAATAGATCCCATTCCCCACCAGCTATTGAGAAAGTACA TTGGCTATGCTCGGCAGTATGTGTACCCAAGGCTATCCACAGAAGCTGCTCGAGTTCTTCAAGATTTTTA CCTTGAGCTCCGGAAACAGAGCCAGAGGTTAAATAGCTCACCAATCACTACCAGGCAGCTGGAATCTTTG ATTCGTCTGACAGAGGCACGAGCAAGGTTGGAATTGAGAGAGGAAGCAACCAAAGAAGACGCTGAGGATA TAGTGGAAATTATGAAATATAGCATGCTAGGAACTTACTCTGATGAATTTGGGAACCTAGATTTTGAGCG ATCCCAGCATGGTTCTGGAATGAGCAACAGGTCAACAGCGAAAAGATTTATTTCTGCTCTCAACAACGTT GCTGAAAGAACTTATAATAATATATTTCAATTTCATCAACTTCGGCAGATTGCCAAAGAACTAAACATTC AGGTTGCTGATTTTGAAAATTTTATTGGATCACTAAATGACCAGGGTTACCTCTTGAAAAAAGGCCCAAA AGTTTACCAGCTTCAAACTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216686 representing NM_182802
Red=Cloning site Green=Tags(s) MNGEYRGRGFGRGRFQSWKRGRGGGNFSGKWREREHRPDLSKTTGKRTSEQTPQFLLSTKTPQSMQSTLD RFIPYKGWKLYFSEVYSDSSPLIEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTEGGEVTNLIPD IATELRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLSNDGETMVNVPHIHARVYNYEPLTQLKNV RANYYGKYIALRGTVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSFTALRSS PLTVTMDWQSIKIQELMSDDQREAGRIPRTIECELVHDLVDSCVPGDTVTITGIVKVSNAEEANSISNSK GQKTKSSEDGCKHGMLMEFSLKDLYAIQEIQAEENLFKLIVNSLCPVIFGHELVKAGLALALFGGSQKYA DDKNRIPIRGDPHILVVGDPGLGKSQMLQAACNVAPRGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGA LVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSEN LKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPL SERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESL IRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNV AERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_182802 |
ORF Size | 2472 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182802.3 |
RefSeq Size | 3710 bp |
RefSeq ORF | 2475 bp |
Locus ID | 84515 |
UniProt ID | Q9UJA3 |
Protein Families | Transcription Factors |
MW | 91.6 kDa |
Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216686L3 | Lenti-ORF clone of MCM8 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 8 (MCM8), transcript variant 2 |
CNY 7,700.00 |
|
RC216686L4 | Lenti-ORF clone of MCM8 (mGFP-tagged)-Human minichromosome maintenance complex component 8 (MCM8), transcript variant 2 |
CNY 7,700.00 |
|
RG216686 | MCM8 (tGFP-tagged) - Human minichromosome maintenance complex component 8 (MCM8), transcript variant 2 |
CNY 6,370.00 |
|
SC126788 | MCM8 (untagged)-Human minichromosome maintenance complex component 8 (MCM8), transcript variant 2 |
CNY 6,040.00 |