NEDL2 (HECW2) (NM_020760) Human Tagged Lenti ORF Clone
CAT#: RC216544L3
- LentiORF®
Lenti ORF clone of Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), Myc-DDK-tagged
"NM_020760" in other vectors (6)
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CNY 17,580.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | NDHSAL; NEDL2 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC216544).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020760 |
ORF Size | 4716 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020760.1 |
RefSeq Size | 6926 bp |
RefSeq ORF | 4719 bp |
Locus ID | 57520 |
UniProt ID | Q9P2P5 |
Protein Families | Druggable Genome |
MW | 175.6 kDa |
Gene Summary | This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216544 | HECW2 (Myc-DDK-tagged)-Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) |
CNY 16,136.00 |
|
RC216544L1 | Lenti ORF clone of Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), Myc-DDK-tagged |
CNY 18,536.00 |
|
RC216544L2 | Lenti ORF clone of Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mGFP tagged |
CNY 17,580.00 |
|
RC216544L4 | Lenti ORF clone of Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mGFP tagged |
CNY 18,536.00 |
|
RG216544 | HECW2 (tGFP-tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) |
CNY 17,736.00 |
|
SC304819 | HECW2 (untagged)-Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) |
CNY 10,760.00 |