CCDC50 (NM_178335) Human Tagged ORF Clone

CAT#: RC216523

CCDC50 (Myc-DDK-tagged)-Human coiled-coil domain containing 50 (CCDC50), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_178335" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Anti-Ymer / CCDC50 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C3orf6; DFNA44; YMER
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216523 representing NM_178335
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAAGTCAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGATTTTGCTGTCC
TGGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATCGAACGTTCA
GCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAGCAGCTCCAAGAGGAAGATCTGAAAGCGCAG
GCCCAGCTCCAGAAGCGCTACAAAGACCTTGAACAACAAGACTGTGAAATTGCTCAGGAAATTCAGGAGA
AGCTGGCTATTGAGGCAGAGAGACGACGCATTCAGGAGAAGAAGGATGAGGACATAGCTCGCCTTTTGCA
AGAAAAGGAGTTACAGGAAGAGAAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCAACCCGTGCTTAT
GCAGATAGTTACTATTATGAAGATGGAGACCAACCAGGGTCAAGGAGGGCCAGGGAATTGGGTTCTGGAT
TCTCAAGACCTTGTAGACTCCAAAGAGATGGAAAGACTGTGAAGCACAAGAAAGAGAAACCAGAACATCC
ACTGGAGAACTTGGAAGAGCCAGAACAACATTGTTCATCGAAGAGATCCCTGTCATCCTCTAGCTCGGGC
AAAGGGAGGGACAATCCCCATATTAACAATGAGCAGCATGAAAGGAAACGGTCCACTCAGGAGAGGCCTC
GGAGACCTCTGCTTCCCACGATCAGTGGTGAAGTGTTTCTGAGCACTGAATGTGATGACTGGGAGACTAA
GATTAACCATCAGACTCGAAATTGGGAAAAACAGTCTCGACACCAAGATCGACTTTCACCCAAGTCCTCA
CAAAAAGCAGGGCTTCACTGCAAGGAAGTTGTATATGGGAGGGACCATGGGCAAGGTGAGCACAGAAAAA
GGAGACACAGGCCCAGGACTCCTCCATTCTCAGAGAGTGAGGAGCAGCTCCACCTCCATGACGCAGGAAT
GAAGCCAAGAGTGATGAAAGAAGCTGTATCTACTCCATCACGAATGGCCCACAGGGATCAGGAATGGTAT
GATGCTGAAATTGCCAGAAAACTGCAAGAAGAAGAACTTTTGGCTACCCAGGTGGACATGAGAGCCGCTC
AAGTAGCTCAAGATGAAGAAATCGCTCGACTTCTAATGGCTGAAGAAAAGAAAGCTTACAAAAAAGCCAA
GGAGCGGGAGAAATCATCTTTGGACAAAAGAAAGCAAGACCCCGAGTGGAAGCCAAAAACAGCTAAAGCA
GCAAATTCCAAGTCAAAAGAGAGTGATGAACCTCACCATTCTAAGAATGAAAGGCCAGCACGGCCACCAC
CACCTATCATGACAGATGGTGAAGATGCGGATTACACTCATTTTACAAACCAGCAGAGTTCCACACGGCA
TTTCTCAAAATCAGAGTCCTCTCATAAAGGTTTTCATTACAAACAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216523 representing NM_178335
Red=Cloning site Green=Tags(s)

MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQ
AQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAY
ADSYYYEDGDQPGSRRARELGSGFSRPCRLQRDGKTVKHKKEKPEHPLENLEEPEQHCSSKRSLSSSSSG
KGRDNPHINNEQHERKRSTQERPRRPLLPTISGEVFLSTECDDWETKINHQTRNWEKQSRHQDRLSPKSS
QKAGLHCKEVVYGRDHGQGEHRKRRHRPRTPPFSESEEQLHLHDAGMKPRVMKEAVSTPSRMAHRDQEWY
DAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKA
ANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_178335
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_178335.3
RefSeq Size 2454 bp
RefSeq ORF 1449 bp
Locus ID 152137
UniProt ID Q8IVM0
MW 56.2 kDa
Gene Summary This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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