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IL24 (NM_006850) Human Tagged ORF Clone
CAT＃: RC216502
- TrueORF®
IL24 (Myc-DDK-tagged)-Human interleukin 24 (IL24), transcript variant 1
ORF Plasmid: tGFP
"NM_006850" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C49A; FISP; IL10B; MDA7; MOB5; ST16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_006850 ORF sequence, RC216502 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATTTTCAACAGAGGCTGCAAAGCCTGTGGACTTTAGCCAGACCCTTCTGCCCTCCTTTGCTGGCG ACAGCCTCTCAAATGCAGATGGTTGTGCTCCCTTGCCTGGGTTTTACCCTGCTTCTCTGGAGCCAGGTA TCAGGGGCCCAGGGCCAAGAATTCCACTTTGGGCCCTGCCAAGTGAAGGGGGTTGTTCCCCAGAAACTG TGGGAAGCCTTCTGGGCTGTGAAAGACACTATGCAAGCTCAGGATAACATCACGAGTGCCCGGCTGCTG CAGCAGGAGGTTCTGCAGAACGTCTCGGATGCTGAGAGCTGTTACCTTGTCCACACCCTGCTGGAGTTC TACTTGAAAACTGTTTTCAAAAACTACCACAATAGAACAGTTGAAGTCAGGACTCTGAAGTCATTCTCT ACTCTGGCCAACAACTTTGTTCTCATCGTGTCACAACTGCAACCCAGTCAAGAAAATGAGATGTTTTCC ATCAGAGACAGTGCACACAGGCGGTTTCTGCTATTCCGGAGAGCATTCAAACAGTTGGACGTAGAAGCA GCTCTGACCAAAGCCCTTGGGGAAGTGGACATTCTTCTGACCTGGATGCAGAAATTCTACAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC216502
Blue=ORF Red=Cloning site Green=Tag(s) MNFQQRLQSLWTLARPFCPPLLATASQMQMVVLPCLGFTLLLWSQVSGAQGQEFHFGPCQVKGVVPQKL WEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFS TLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC216502 also available, TP316502M |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006850 |
| ORF Size | 618 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006850.3, NP_006841.1 |
| RefSeq Size | 1976 bp |
| RefSeq ORF | 621 bp |
| Locus ID | 11009 |
| UniProt ID | Q13007 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
| MW | 23.8 kDa |
| Gene Summary | This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bis-indole derived nuclear receptor 4A1 (NR4A1) antagonists inhibit TGFβ-induced invasion of embryonal rhabdomyosarcoma cells
,Shrestha, R;Mohankumar, K;Safe, S;,
American journal of cancer research
,PubMed ID 32905449
[IL24]
|
|
Interleukin-24 (IL-24) Is Suppressed By PAX3-FOXO1 and Is A Novel Therapy For Rhabdomyosarcoma
,Lacey, A;Hedrick, E;Cheng, Y;Mohankumar, K;Warren, M;Safe, S;,
Mol. Cancer Ther.
,PubMed ID 30190424
[IL24]
|
|
Crystal Structure of the Labile Complex of IL-24 with the Extracellular Domains of IL-22R1 and IL-20R2
,Lubkowski, J;Sonmez, C;Smirnov, SV;Anishkin, A;Kotenko, SV;Wlodawer, A;,
J. Immunol.
,PubMed ID 30111632
[IL24]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216502L1 | Lenti ORF clone of Human interleukin 24 (IL24), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216502L2 | Lenti ORF clone of Human interleukin 24 (IL24), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216502L3 | Lenti ORF clone of Human interleukin 24 (IL24), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216502L4 | Lenti ORF clone of Human interleukin 24 (IL24), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG216502 | IL24 (tGFP-tagged) - Human interleukin 24 (IL24), transcript variant 1 |
CNY 4,000.00 |
|
| SC303831 | IL24 (untagged)-Human interleukin 24 (IL24), transcript variant 1 |
CNY 3,990.00 |
