CBX4 (NM_003655) Human Tagged ORF Clone
CAT#: RC216414
- TrueORF®
CBX4 (Myc-DDK-tagged)-Human chromobox homolog 4 (CBX4)
ORF Plasmid: tGFP
"NM_003655" in other vectors (6)
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CNY 6,256.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | NBP16; PC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216414 representing NM_003655
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCCAGCTGTTGGCGAGCACGTCTTCGCGGTGGAGAGCATCGAGAAGAAGCGGATCCGCAAGG GCAGAGTGGAGTATCTGGTGAAATGGAGAGGCTGGTCGCCCAAATATAACACGTGGGAACCGGAGGAGAA CATCCTGGACCCCAGGCTGCTGATCGCCTTCCAGAACAGGGAACGGCAGGAGCAGCTGATGGGATATCGG AAGAGAGGGCCGAAGCCCAAACCGCTAGTGGTGCAGGTGCCTACCTTTGCCCGTCGTTCCAATGTCCTGA CCGGCCTCCAGGACTCCTCCACTGACAACCGTGCCAAGCTGGATTTGGGCGCGCAGGGGAAGGGCCAGGG GCATCAGTACGAGCTCAACAGCAAGAAGCACCACCAGTACCAGCCGCACAGCAAGGAGCGGGCGGGCAAG CCCCCGCCGCCGGGCAAGAGCGGCAAGTACTACTACCAGCTCAACAGCAAGAAGCACCACCCCTACCAGC CCGACCCCAAAATGTACGACCTGCAGTACCAGGGCGGCCACAAGGAGGCGCCCAGCCCCACCTGCCCGGA CCTGGGGGCCAAGAGCCACCCGCCCGACAAGTGGGCGCAAGGTGCGGGGGCCAAAGGCTACCTGGGGGCG GTGAAGCCCCTGGCCGGTGCGGCGGGTGCTCCAGGCAAAGGCTCCGAGAAGGGCCCCCCCAACGGAATGA TGCCGGCCCCCAAAGAGGCTGTGACGGGCAACGGGATTGGGGGCAAGATGAAGATAGTCAAGAACAAGAA CAAGAACGGACGCATCGTGATCGTGATGAGCAAATACATGGAGAACGGCATGCAGGCGGTGAAGATCAAG TCCGGCGAGGTGGCAGAGGGGGAGGCTCGCTCCCCCAGCCACAAGAAGCGGGCAGCCGACGAGCGCCACC CTCCTGCCGACAGGACTTTTAAAAAGGCGGCGGGCGCAGAGGAGAAGAAGGTGGAGGCGCCGCCCAAGAG GAGGGAGGAGGAGGTGTCCGGGGTTAGCGATCCGCAGCCCCAGGATGCCGGCTCCCGCAAGCTGTCCCCG ACCAAGGAGGCCTTTGGAGAGCAGCCCCTGCAGCTCACCACCAAGCCCGACCTGCTTGCCTGGGACCCGG CCCGGAACACGCACCCGCCCTCACACCACCCGCACCCGCACCCCCATCACCACCACCACCACCACCACCA CCACCACCACGCCGTCGGCCTGAATCTCTCCCACGTGCGCAAGCGCTGCCTCTCCGAGACCCACGGCGAG CGCGAGCCCTGCAAGAAGCGGCTGACTGCGCGCAGCATCAGCACCCCCACCTGCCTGGGGGGCAGCCCAG CCGCTGAGCGCCCGGCCGACCTGCCACCAGCCGCCGCCCTCCCGCAGCCCGAGGTCATCCTGCTAGACTC AGACCTGGATGAACCCATAGACTTGCGCTGCGTCAAGACGCGCAGCGAGGCCGGGGAGCCGCCCAGCTCC CTCCAGGTGAAGCCCGAGACACCGGCGTCGGCGGCGGTGGCGGTGGCGGCGGCAGCGGCACCCACCACGA CGGCGGAGAAGCCTCCAGCCGAGGCCCAGGACGAACCTGCAGAGTCGCTGAGCGAGTTCAAGCCCTTCTT TGGGAATATAATTATCACCGACGTCACCGCGAACTGCCTCACCGTTACTTTCAAGGAGTACGTGACGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216414 representing NM_003655
Red=Cloning site Green=Tags(s) MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYR KRGPKPKPLVVQVPTFARRSNVLTGLQDSSTDNRAKLDLGAQGKGQGHQYELNSKKHHQYQPHSKERAGK PPPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEAPSPTCPDLGAKSHPPDKWAQGAGAKGYLGA VKPLAGAAGAPGKGSEKGPPNGMMPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIK SGEVAEGEARSPSHKKRAADERHPPADRTFKKAAGAEEKKVEAPPKRREEEVSGVSDPQPQDAGSRKLSP TKEAFGEQPLQLTTKPDLLAWDPARNTHPPSHHPHPHPHHHHHHHHHHHHAVGLNLSHVRKRCLSETHGE REPCKKRLTARSISTPTCLGGSPAAERPADLPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSS LQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003655 |
ORF Size | 1680 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003655.3 |
RefSeq Size | 2655 bp |
RefSeq ORF | 1683 bp |
Locus ID | 8535 |
UniProt ID | O00257 |
Domains | CHROMO |
Protein Families | Druggable Genome, Transcription Factors |
MW | 61.8 kDa |
Gene Summary | E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed:12679040). Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed:22825850).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216414L1 | Lenti-ORF clone of CBX4 (Myc-DDK-tagged)-Human chromobox homolog 4 (CBX4) |
CNY 8,656.00 |
|
RC216414L2 | Lenti-ORF clone of CBX4 (mGFP-tagged)-Human chromobox homolog 4 (CBX4) |
CNY 6,840.00 |
|
RC216414L3 | Lenti-ORF clone of CBX4 (Myc-DDK-tagged)-Human chromobox homolog 4 (CBX4) |
CNY 6,840.00 |
|
RC216414L4 | Lenti-ORF clone of CBX4 (mGFP-tagged)-Human chromobox homolog 4 (CBX4) |
CNY 8,656.00 |
|
RG216414 | CBX4 (tGFP-tagged) - Human chromobox homolog 4 (CBX4) |
CNY 7,856.00 |
|
SC117841 | CBX4 (untagged)-Human chromobox homolog 4 (CBX4) |
CNY 6,264.00 |