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FBXO43 (NM_001029860) Human Tagged ORF Clone
CAT＃: RC216347
FBXO43 (Myc-DDK-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2
ORF Plasmid: tGFP
"NM_001029860" in other vectors (4)
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CNY 5,184.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EMI2; ERP1; FBX43 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216347 representing NM_001029860
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTTAAAGACAAAGATGAGAGAATTTCTTGTTTGGAAGCCTACGTAACTTTGACATCTAAGAGCT CAAGATTTACTGATGAAACAGAGATTTTGAAGATGTCGCAAAGGCACTCAGGTCAAGCTGGCACTGAAGC AGGAAATGGGGCGGACTCTCCTCCAATTGTCAACTCCAAGTACTCCACCTTCAGAGATTTTTGTTCCACA TCTTCATTTCAAGATAGTGGCTACAATGAGTTAAAATCTTGTAGCTTTGATAATATAGATAAAGAATATC TTGGAAAGAAAGAAAAAGGCCCAACATTACTCTATGAGCACCCTGAAACTTCAGGCCTGGGCTTAACACA TCCTTTAGAATCTCCCACTCAAAAAAAGAAATGTATCTTGCCTAGAAAGGAAAAGGATAAAACCCCAGAA CTTTGTGAAACACCTAAAATCAGTGGGAAAAAATGTTTACCTCGCAGAAGGTTGAATGTATCTTTCGCTC TTCTAAAAGGGGACTTTGAATCACAAAATAGTTCTTTAGAAAGTAGTATAAGCCAAGTTATCAACTTAGA AAAAAATATTCCAAGCAGTGCTTCAGGTTTTTCCAGGGCAAATAATTTTAGCCCTTTAGTTACTAGCACT TTAAAAACAGAAGAAGTGACTTCATGCAGTCAAAAATTGAGGCTTAATTTTTCTCAGCAAAAGACTTCCA CAATTGATGATTCCAAAGATGATTGTAGCCTATTTGAAGTTGAATGTATATCTCCAATTCAGGGCAATAA TTTTAAAGACTCTATCACACATGACTTTAGTGATAGCAGTTTATGCATTAATGATGAGAATGCATGTCCA GAGCTCCTGGGCTCCTCTGTTAGTGGAACAACTTGTGGAACAGATGAGGACATATTTGTGACTCCGATAA GTAATCTTGTGGCAAACATTAGATTTAACGCAAGTCAAATACTTTCTCCTTCACCTGAAGTGAGAGGCAG TATTTCAACGCCTGAAGACAGTGGTTTTAACTCACTTAGCTTGGAGAAATCAGAAGATTCCCTGTCTGAC CAGGAGGGTTCTTTTCAAGAACTACTGCAGAAACATAAGGGGACTCCCAAAGTTGGGGACACCATAAGAA AGACAAGACATCTTGGAAGGTCGAGAAGACTGTCCACCCTTCGGGAACAAAGCTCGCAGTCAGAGACAGA AGAGGAAAAGCAGATTGTCCACCCTGACTCTGAAAAAAGAGCAGCAGCTGCTTCTGCCATCTCAGAGGGT CAGCTGAGTAGTGATGAGAGTGGGGATTTGACCTTTAGCTTAAAGAATTTATCAAAGACCCCAGCCTTGC AATTGGTACATGAGCTGTTCATGAAAAGCAAGAGGAAAAGATTACAGGAAAATAGTGGACATGAATTCTT AGAGCAAGGGGATGGGGAGAAAATAGCTGTACTGCAGTGTATACTTGCAGGACTGATCGGCAAGAAAATG GGTATAGAAAAACTGGACATCTTAACAGAATTAAAATATAGAAATTTAAAGCATATTCTTGCTATGGTTT TAGAGTCCTTGACCGCAGAGAGCCTATGCAGTGTTTGGAAAGTAAGCAGAAATTGGCGTGAAATTGTTGT TCAAGATAAAAATGCAAATCGGAGGAGGAAATTTTATATCACACAACTGAAAACAGATTCTGAGGGGGCT GTATTAAATGTCGAGGATGCTGCCACTCGGCTCCAGCTTTTAAATCGCTCAGCTTTAAGATCTGTGCAGG CACAGGCTAGGATACCTGGTTCTCAGAGAGAGCAAGGGTCAACATTATCTCCCTGGGGAGAAGTTTTGAC ACCTCTAGCAAGCTCTTCTGTTACTCACTTAAGTAGTAAACAGGAAGAATATGTTAAGGTTGCCAAAACA CTTTTTACTGATGAAGCATTAAAACCTTGCCCAAGGTGCCAGTCCCCTGCTAAGTACCAGCCATATAAGA AAAGGGGACTGTGTAGCCGAACAGCCTGTGGTTTTGACTTTTGTGTGTTATGTCTGTGTGCTTATCATGG GTCTGAAGAATGTAGTAGAGGAGCAGCAAAGCCAAGAAATAGAAAAGATGCTCTCCCAGGAAGTGCCCAG AGTAAGCGGAATTTAAAACGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216347 representing NM_001029860
Red=Cloning site Green=Tags(s) MSFKDKDERISCLEAYVTLTSKSSRFTDETEILKMSQRHSGQAGTEAGNGADSPPIVNSKYSTFRDFCST SSFQDSGYNELKSCSFDNIDKEYLGKKEKGPTLLYEHPETSGLGLTHPLESPTQKKKCILPRKEKDKTPE LCETPKISGKKCLPRRRLNVSFALLKGDFESQNSSLESSISQVINLEKNIPSSASGFSRANNFSPLVTST LKTEEVTSCSQKLRLNFSQQKTSTIDDSKDDCSLFEVECISPIQGNNFKDSITHDFSDSSLCINDENACP ELLGSSVSGTTCGTDEDIFVTPISNLVANIRFNASQILSPSPEVRGSISTPEDSGFNSLSLEKSEDSLSD QEGSFQELLQKHKGTPKVGDTIRKTRHLGRSRRLSTLREQSSQSETEEEKQIVHPDSEKRAAAASAISEG QLSSDESGDLTFSLKNLSKTPALQLVHELFMKSKRKRLQENSGHEFLEQGDGEKIAVLQCILAGLIGKKM GIEKLDILTELKYRNLKHILAMVLESLTAESLCSVWKVSRNWREIVVQDKNANRRRKFYITQLKTDSEGA VLNVEDAATRLQLLNRSALRSVQAQARIPGSQREQGSTLSPWGEVLTPLASSSVTHLSSKQEEYVKVAKT LFTDEALKPCPRCQSPAKYQPYKKRGLCSRTACGFDFCVLCLCAYHGSEECSRGAAKPRNRKDALPGSAQ SKRNLKRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001029860 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001029860.4 |
| RefSeq Size | 3211 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 286151 |
| UniProt ID | Q4G163 |
| Protein Pathways | Oocyte meiosis |
| MW | 78.2 kDa |
| Gene Summary | Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216347L3 | Lenti-ORF clone of FBXO43 (Myc-DDK-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2 |
CNY 7,584.00 |
|
| RC216347L4 | Lenti-ORF clone of FBXO43 (mGFP-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2 |
CNY 9,310.00 |
|
| RG216347 | FBXO43 (tGFP-tagged) - Human F-box protein 43 (FBXO43), transcript variant 2 |
CNY 8,170.00 |
|
| SC318190 | FBXO43 (untagged)-Human F-box protein 43 (FBXO43), transcript variant 2 |
CNY 13,680.00 |
