FBXO44 (NM_033182) Human Tagged ORF Clone
CAT#: RC216126
FBXO44 (Myc-DDK-tagged)-Human F-box protein 44 (FBXO44), transcript variant 1
ORF Plasmid: tGFP
"NM_033182" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FBG3; FBX6A; FBX30; Fbx44; Fbxo6a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216126 representing NM_033182
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGGGAACATCAACGAGCTGCCCGAGAACATCCTGCTGGAGCTGTTCACGCACGTGCCCGCCC GCCAGCTGCTGCTGAACTGCCGCCTGGTCTGCAGCCTCTGGCGGGACCTCATCGACCTCGTGACCCTCTG GAAACGCAAGTGCCTGCGAGAGGGCTTCATCACTGAGGACTGGGACCAGCCCGTGGCCGACTGGAAGATC TTCTACTTCTTACGGAGCCTGCACAGGAACCTCCTGCACAACCCGTGCGCTGAAGAGGGGTTCGAGTTCT GGAGCCTGGATGTGAATGGAGGCGATGAGTGGAAGGTGGAGGATCTCTCTCGAGACCAGAGGAAGGAATT CCCCAATGACCAGGTCAAGAAATACTTCGTTACTTCATATTACACCTGCCTCAAGTCCCAGGTGGTGGAC CTCAAGGCCGAAGGGTATTGGGAGGAGCTGATGGATACCACACGGCCGGACATCGAGGTCAAGGACTGGT TCGCAGCCAGGCCAGATTGCGGGTCCAAGTACCAGCTGTGCGTTCAGCTCCTGTCGTCCGCGCACGCGCC TCTGGGGACCTTCCAGCCAGACCCGGCGACCATCCAGCAGAAGAGCGATGCCAAGTGGAGGGAGGTCTCC CACACATTCTCCAACTACCCGCCCGGCGTCCGCTACATCTGGTTTCAGCACGGCGGCGTGGACACTCATT ACTGGGCCGGCTGGTACGGCCCGAGGGTCACCAACAGCAGCATCACCATCGGGCCCCCGCTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216126 representing NM_033182
Red=Cloning site Green=Tags(s) MAVGNINELPENILLELFTHVPARQLLLNCRLVCSLWRDLIDLVTLWKRKCLREGFITEDWDQPVADWKI FYFLRSLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVKKYFVTSYYTCLKSQVVD LKAEGYWEELMDTTRPDIEVKDWFAARPDCGSKYQLCVQLLSSAHAPLGTFQPDPATIQQKSDAKWREVS HTFSNYPPGVRYIWFQHGGVDTHYWAGWYGPRVTNSSITIGPPLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033182 |
ORF Size | 765 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033182.7 |
RefSeq Size | 2948 bp |
RefSeq ORF | 768 bp |
Locus ID | 93611 |
UniProt ID | Q9H4M3 |
Domains | F-box, FBA |
Protein Families | Druggable Genome |
MW | 29.6 kDa |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Several alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Feb 2015] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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E3 Ligase RNF126 Directly Ubiquitinates Frataxin, Promoting Its Degradation: Identification of a Potential Therapeutic Target for Friedreich Ataxia
,Benini, M;Fortuni, S;Condò, I;Alfedi, G;Malisan, F;Toschi, N;Serio, D;Massaro, DS;Arcuri, G;Testi, R;Rufini, A;,
Cell Rep
,PubMed ID 28228265
[FBXO44]
|
The F-box Protein FBXO44 Mediates BRCA1 Ubiquitination and Degradation
,Yunzhe Lu, Jiezhi Li, Dongmei Cheng, Balaji Parameswaran, Shaohua Zhang, Zefei Jiang, P. Renee Yew, Junmin Peng, Qinong Ye, and Yanfen Hu,
J. Biol. Chem., Nov 2012; 287: 41014 - 41022.
[FBXO44]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216126L1 | Lenti ORF clone of Human F-box protein 44 (FBXO44), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC216126L2 | Lenti ORF clone of Human F-box protein 44 (FBXO44), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC216126L3 | Lenti ORF clone of Human F-box protein 44 (FBXO44), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216126L4 | Lenti ORF clone of Human F-box protein 44 (FBXO44), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG216126 | FBXO44 (tGFP-tagged) - Human F-box protein 44 (FBXO44), transcript variant 1 |
CNY 4,000.00 |
|
SC311243 | FBXO44 (untagged)-Human F-box protein 44 (FBXO44), transcript variant 1 |
CNY 2,400.00 |