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CGK2 (PRKG2) (NM_006259) Human Tagged ORF Clone
CAT＃: RC215879
PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)
ORF Plasmid: tGFP
"NM_006259" in other vectors (7)
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CNY 5,584.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cGK2; cGKII; PKG2; PRKGR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215879 representing NM_006259
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAACCTCACCACTG ATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAGGATGCTGAGATCCAGGAGCG GGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAGCAGACTGTGGCCATTGCTGAACTCACAGAG GAGCTCCAGAACAAGTGCATCCAGCTGAACAAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGC TTCAGGCCTCTCCAGATAAAGTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAG CAGGAGGGGAGCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACCCCCT GAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACAGATGCCCTTA ATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATGGTGGAATGCATGTATGGGAG AAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAACCAGGAAACCATATCTTTGTGCTGGCAGAG GGTCGACTAGAGGTGTTCCAAGGGGAGAAATTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGC TTGCCATTTTATACAATTGTACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACATGGGCACT AGATCGAGAGGTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAATACAGAAACTTC CTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATTGACTGCTTGGAAG TGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGAAGTACCTTTTTCATTTTGGC AAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCATGATCAACCACAGCTGATAAAAACACTGCAG AAAGGAGAATACTTTGGAGAAAAAGCTCTTATCAGTGATGATGTCAGGTCAGCTAACATTATTGCTGAAG AAAATGATGTTGCATGCCTGGTTATAGATCGAGAAACATTCAACCAAACTGTCGGTACATTTGAAGAGCT GCAAAAATACCTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAAAGACATGCGAAGCGGTCC ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAGGTGGCCAGAT TTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGCGTTGGTGGGTTCGGAAGAGT TGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGAAGTGTATAAGGAAGAAGCACATAGTT GACACCAAGCAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTG TGAAATTATATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGA GCTCTGGAGTATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCTTGTGTG ACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAAAACTTAATTC TAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAAATAGGGTCTGGACAGAAAAC ATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAAGTCATTCTCAACAAGGGACATGACTTCAGT GTGGATTTCTGGTCACTGGGAATTCTAGTGTATGAGCTCCTAACGGGCAACCCACCCTTTTCTGGGGTTG ACCAAATGATGACCTACAATTTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGGAAGATAACACG ACGACCTGAGGATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGAAATCTGAAGAAT GGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTGAAAGCACGGAGCC TTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTACTTTGACAAATATCCTCCTGA AAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAAGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215879 representing NM_006259
Red=Cloning site Green=Tags(s) MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTE ELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPP EFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAE GRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNF LRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQ KGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRS MSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIV DTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACV TEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFS VDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKN GINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006259 |
| ORF Size | 2286 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006259.2 |
| RefSeq Size | 3328 bp |
| RefSeq ORF | 2289 bp |
| Locus ID | 5593 |
| UniProt ID | Q13237 |
| Domains | cNMP, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, Long-term depression, Olfactory transduction |
| MW | 87.3 kDa |
| Gene Summary | This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Biallelic cGMP-dependent type II protein kinase gene (PRKG2) variants cause a novel acromesomelic dysplasia
,Díaz-González, F;Wadhwa, S;Rodriguez-Zabala, M;Kumar, S;Aza-Carmona, M;Sentchordi-Montané, L;Alonso-Blanco, M;Ahmad, I;Zahra, S;Kumar, D;Kushwah, N;Shamim, U;Sait, H;Kapoor, S;Rodan-Martin, MB;Nishimura, G;Offiah, AC;Faruq, M;Heath, KE;,
J Med Genet
,PubMed ID 33106379
[CGK2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215879L1 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), Myc-DDK-tagged |
CNY 7,984.00 |
|
| RC215879L2 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), mGFP tagged |
CNY 7,700.00 |
|
| RC215879L3 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC215879L4 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), mGFP tagged |
CNY 7,984.00 |
|
| RG215879 | PRKG2 (tGFP-tagged) - Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 7,184.00 |
|
| SC116254 | PRKG2 (untagged)-Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 5,592.00 |
|
| SC323491 | PRKG2 (untagged)-Kinase deficient mutant (K482M) of Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 5,592.00 |
