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KDM2B (NM_001005366) Human Tagged ORF Clone
CAT＃: RC215776
KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2
ORF Plasmid: tGFP
"NM_001005366" in other vectors (4)
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CNY 8,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CXXC2; Fbl10; FBXL10; JHDM1B; PCCX2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215776 representing NM_001005366
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGAGAAAGACTCTGGAAGAAGATTGCGCCCGATTGACCGCCAGCGATACGACGAGAACGAGG ACTTGTCGGACGTGGAGGAGATCGTCAGCGTCCGCGGCTTCAGCCTGGAGGAGAAGCTTCGCAGCCAGCT GTACCAGGGGGACTTCGTGCACGCCATGGAGGGCAAAGATTTCAACTATGAGTACGTACAGAGAGAAGCT CTCAGGGTTCCCCTGATATTTCGAGAAAAGGATGGACTGGGAATTAAGATGCCTGACCCTGATTTCACAG TCCGAGACGTCAAACTCCTAGTGGGGAGCCGGCGGCTTGTGGACGTGATGGATGTGAACACCCAGAAGGG CACGGAGATGAGCATGTCCCAGTTTGTGCGTTACTACGAGACGCCCGAGGCCCAGCGGGACAAGCTGTAC AACGTCATCAGCCTAGAGTTCAGCCACACCAAGCTGGAGCACTTGGTCAAGCGTCCGACTGTGGTAGACC TGGTGGACTGGGTGGACAACATGTGGCCCCAGCATCTGAAGGAGAAGCAGACAGAAGCCACGAACGCCAT TGCAGAGATGAAGTACCCGAAAGTGAAAAAGTACTGTCTGATGAGCGTGAAAGGTTGTTTCACCGACTTC CACATCGACTTTGGAGGCACTTCCGTTTGGTACCATGTTTTCCGGGGTGGGAAGATTTTTTGGCTGATTC CTCCAACGCTGCACAATTTGGCGCTGTACGAGGAGTGGGTGCTGTCAGGCAAACAGAGTGACATCTTTCT GGGAGACCGTGTGGAACGATGCCAAAGAATTGAGCTGAAGCAGGGCTACACATTTTTCATCCCTTCCGGT TGGATCCATGCCGTCTACACCCCTGTAGACTCTTTGGTGTTCGGCGGAAACATCCTGCACAGCTTTAACG TGCCCATGCAGCTGCGGATCTACGAGATCGAGGACAGGACGCGGGTGCAGCCCAAATTCCGTTACCCCTT CTACTATGAGATGTGCTGGTATGTCCTGGAGAGATACGTGTACTGTGTGACCCAGCGCTCCCACCTCACT CAGGAATACCAGAGGGAGTCGATGCTTATTGATGCCCCGAGGAAGCCCAGCATAGACGGCTTCTCTTCGG ATTCCTGGCTGGAGATGGAGGAGGAGGCCTGTGATCAGCAGCCTCAGGAGGAGGAGGAGAAGGACGAGGA GGGCGAGGGCAGGGACAGGGCACCCAAACCGCCCACCGATGGCTCCACTTCACCCACCAGCACGCCCTCT GAGGACCAGGAGGCCCTCGGGAAGAAGCCCAAAGCACCTGCCCTGCGATTCCTCAAAAGGACTTTGTCTA ATGAGTCGGAGGAAAGTGTGAAGTCCACCACATTGGCCGTAGACTACCCCAAGACCCCCACCGGCTCTCC CGCCACGGAGGTCTCTGCCAAATGGACCCATCTCACTGAGTTTGAACTGAAGGGCCTGAAAGCTCTGGTG GAGAAACTGGAATCCCTCCCGGAGAACAAGAAGTGTGTCCCCGAGGGCATCGAGGACCCCCAGGCACTCC TGGAGGGTGTGAAGAACGTCCTGAAGGAGCACGCAGATGATGACCCTAGTCTGGCCATCACTGGGGTCCC TGTGGTGACTTGGCCAAAGAAGACTCCAAAGAACCGGGCTGTGGGTCGGCCCAAGGGGAAGCTGGGCCCG GCCTCCGCGGTGAAGTTGGCCGCCAACCGGACAACGGCAGGAGCTCGGCGGCGCCGGACGCGATGCCGCA AGTGCGAGGCCTGCCTGCGGACCGAGTGCGGAGAGTGCCACTTCTGCAAGGACATGAAGAAGTTCGGGGG CCCCGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGCCAGTGCTGCCCCACACCGCCGTG TGCCTTGTGTGTGGCGAGGCGGGGAAGGAAGACACGGTGGAAGAGGAGGAAGGCAAGTTTAACCTCATGC TCATGGAGTGCTCCATCTGCAATGAAATCATCCACCCTGGATGCCTTAAGATTAAGGAGTCAGAGGGTGT GGTCAACGACGAGCTTCCAAACTGCTGGGAGTGTCCGAAGTGTAACCACGCCGGCAAGACCGGGAAACAA AAGCGTGGCCCTGGCTTTAAGTACGCCTCCAACCTGCCCGGCTCCCTGCTCAAGGAGCAGAAGATGAACC GGGACAACAAGGAAGGGCAGGAACCTGCCAAGCGGAGGAGTGAGTGTGAGGAGGCGCCCCGGCGCAGGTC GGATGAGCACTCGAAGAAGGTGCCGCCGGACGGCCTTCTGCGCAGAAAGTCTGACGACGTGCACCTGAGG AAGAAGCGGAAATACGAGAAGCCCCAGGAGCTGAGTGGACGCAAGCGGCTCAAACCTGGCAAAGAAGATA AGCTTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCCGAGGACCGCATGGCGCTGGCCAACAAGCCCCT CCGGCGCTTCAAGCAGGAACCCGAGGACGAACTGCCCGAGGCGCCCCCCAAGACCAGGGAGAGCGACCAC TCCCGCTCCAGCTCCCCCACCGCGGGACCCAGCACCGAAGGGGCCGAGGGCCCGGAGGAGAAGAAGAAGG TGAAGATGCGCCGGAAGCGGCGGCTTCCCAACAAGGAGCTGAGCAGGGAGCTGAGCAAGGAGCTCAACCA CGAGATCCAGAGGACGGAGAACAGCCTGGCCAACGAGAACCAGCAGCCCATCAAGTCGGAGCCTGAGAGC GAGGGCGAGGAGCCCAAGCGGCCCCCGGGCATCTGCGAGCGTCCCCACCGCTTCAGCAAGGGGCTCAACG GCACCCCCCGGGAGCTGCGGCACCAGCTGGGGCCCAGCCTGCGCAGCCCGCCCCGTGTCATCTCCCGGCC CCCACCCTCCGTGTCCCCGCCCAAGTGTATCCAGATGGAGCGCCATGTGATCCGGCCACCCCCCATCAGC CCCCCGCCTGACTCGCTACCCCTGGACGATGGGGCAGCCCACGTCATGCACAGGGAGGTGTGGATGGCCG TCTTCAGCTACCTCAGCCACCAAGACCTGTGTGTGTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTG CTGCGATAAGCGGTTGTGGACCCGCATTGACCTGAACCACTGCAAGTCTATCACACCCCTGATGCTGAGT GGCATCATCCGGCGACAGCCCGTCTCCCTCGACCTCAGCTGGACCAATATCTCCAAGAAGCAGCTGAGCT GGCTCATCAACCGGCTGCCTGGGCTCCGGGACTTGGTGCTGTCAGGCTGCTCATGGATCGCGGTCTCGGC CCTTTGCAGCTCCAGTTGTCCGCTGCTCCGGACCCTGGATGTCCAGTGGGTGGAGGGACTAAAGGATGCC CAGATGCGGGATCTCCTGTCCCCGCCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCC GGAACATCGTGGAGCTGCGCCTGGCAGGCCTGGACATCACAGATGCCTCCCTGCGGCTCATCATCCGCCA CATGCCCCTGCTCTCCAAGCTCCACCTCAGTTACTGTAACCACGTCACCGACCAGTCTATCAACCTGCTC ACTGCTGTTGGCACCACCACCCGAGACTCCTTAACCGAGATCAACCTGTCTGACTGCAATAAGGTCACTG ATCAGTGCCTGTCCTTCTTCAAACGCTGTGGAAACATCTGTCATATTGACCTGAGGTACTGCAAGCAAGT CACCAAGGAAGGCTGTGAGCAGTTCATAGCCGAGATGTCTTCTTTCCAAGGGAGAAGCTGTTCAACCACC CGTTTGGGGGATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215776 representing NM_001005366
Red=Cloning site Green=Tags(s) MEAEKDSGRRLRPIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREA LRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLY NVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNAIAEMKYPKVKKYCLMSVKGCFTDF HIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSG WIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSHLT QEYQRESMLIDAPRKPSIDGFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTPS EDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALV EKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDPSLAITGVPVVTWPKKTPKNRAVGRPKGKLGP ASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLPHTAV CLVCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCLKIKESEGVVNDELPNCWECPKCNHAGKTGKQ KRGPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSECEEAPRRRSDEHSKKVPPDGLLRRKSDDVHLR KKRKYEKPQELSGRKRLKPGKEDKLFRKKRRSWKNAEDRMALANKPLRRFKQEPEDELPEAPPKTRESDH SRSSSPTAGPSTEGAEGPEEKKKVKMRRKRRLPNKELSRELSKELNHEIQRTENSLANENQQPIKSEPES EGEEPKRPPGICERPHRFSKGLNGTPRELRHQLGPSLRSPPRVISRPPPSVSPPKCIQMERHVIRPPPIS PPPDSLPLDDGAAHVMHREVWMAVFSYLSHQDLCVCMRVCRTWNRWCCDKRLWTRIDLNHCKSITPLMLS GIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDA QMRDLLSPPTDNRPGQMDNRSKLRNIVELRLAGLDITDASLRLIIRHMPLLSKLHLSYCNHVTDQSINLL TAVGTTTRDSLTEINLSDCNKVTDQCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSSFQGRSCSTT RLGDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005366 |
| ORF Size | 3795 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001005366.2 |
| RefSeq Size | 5236 bp |
| RefSeq ORF | 3798 bp |
| Locus ID | 84678 |
| UniProt ID | Q8NHM5 |
| Protein Families | Druggable Genome |
| MW | 144.6 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215776L3 | Lenti-ORF clone of KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2 |
CNY 14,540.00 |
|
| RC215776L4 | Lenti-ORF clone of KDM2B (mGFP-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2 |
CNY 11,208.00 |
|
| RG215776 | KDM2B (tGFP-tagged) - Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2 |
CNY 13,870.00 |
|
| SC300925 | KDM2B (untagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2 |
CNY 24,320.00 |
