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DAPP1 (NM_014395) Human Tagged ORF Clone
CAT＃: RC215474
DAPP1 (Myc-DDK-tagged)-Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1)
ORF Plasmid: tGFP
"NM_014395" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BAM32 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215474 representing NM_014395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGAGCAGAACTTCTAGAAGGGAAGATGAGCACCCAGGATCCCTCAGATCTGTGGAGCAGATCCG ATGGAGAGGCTGAGCTGCTCCAGGACTTGGGGTGGTATCACGGCAACCTCACACGCCATGCTGCTGAAGC TCTTCTCCTCTCAAATGGATGTGACGGCAGCTACCTTCTGAGGGACAGCAATGAGACCACCGGGCTGTAC TCTCTCTCTGTGAGGGCCAAAGATTCTGTTAAACACTTTCATGTTGAATATACTGGATATTCATTTAAAT TTGGCTTTAATGAATTCTCATCTTTGAAGGATTTTGTCAAGCATTTTGCAAATCAGCCTTTGATTGGAAG CGAGACAGGCACTCTGATGGTTCTAAAACATCCCTACCCAAGAAAAGTGGAAGAACCCTCCATTTATGAA TCTGTCCGGGTTCACACAGCAATGCAGACAGGAAGAACAGAAGATGACCTTGTGCCCACAGCACCTTCTC TGGGCACCAAAGAAGGTTACCTCACCAAACAGGGAGGCCTGGTCAAGACCTGGAAAACAAGATGGTTTAC TCTGCACAGGAATGAACTGAAATACTTCAAAGACCAGATGTCACCAGAACCAATTCGGATCCTAGACCTA ACAGAATGTTCAGCTGTACAATTCGATTATTCACAAGAAAGGGTAAACTGTTTTTGTTTGGTATTTCCAT TCAGGACATTTTATCTCTGTGCAAAGACCGGAGTAGAAGCTGATGAGTGGATCAAGATATTACGCTGGAA ATTGTCACAAATAAGAAAACAGCTCAACCAAGGGGAAGGCACGATCCGATCTCGGTCGTTCATCTTTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215474 representing NM_014395
Red=Cloning site Green=Tags(s) MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLLSNGCDGSYLLRDSNETTGLY SLSVRAKDSVKHFHVEYTGYSFKFGFNEFSSLKDFVKHFANQPLIGSETGTLMVLKHPYPRKVEEPSIYE SVRVHTAMQTGRTEDDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLHRNELKYFKDQMSPEPIRILDL TECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSQIRKQLNQGEGTIRSRSFIFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014395 |
| ORF Size | 840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014395.3 |
| RefSeq Size | 2953 bp |
| RefSeq ORF | 843 bp |
| Locus ID | 27071 |
| UniProt ID | Q9UN19 |
| Domains | SH2, PH |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | B cell receptor signaling pathway |
| MW | 32.6 kDa |
| Gene Summary | May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215474L1 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC215474L2 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mGFP tagged |
CNY 5,890.00 |
|
| RC215474L3 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215474L4 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mGFP tagged |
CNY 5,890.00 |
|
| RG215474 | DAPP1 (tGFP-tagged) - Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1) |
CNY 4,000.00 |
|
| SC108632 | DAPP1 (untagged)-Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1) |
CNY 2,400.00 |
