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MYO3A (NM_017433) Human Tagged ORF Clone
CAT＃: RC215401
- TrueORF®
MYO3A (Myc-DDK-tagged)-Human myosin IIIA (MYO3A)
ORF Plasmid: tGFP
"NM_017433" in other vectors (4)
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CNY 16,150.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNB30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215401 representing NM_017433
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCCATTAATTGGAAAAACAATCATCTTTGATAACTTTCCTGATCCTTCTGATACATGGGAAATCA CTGAGACAATTGGCAAAGGAACTTATGGGAAAGTTTTTAAAGTATTGAATAAGAAAAATGGCCAAAAAGC AGCAGTCAAAATTCTTGATCCAATTCACGATATTGACGAAGAGATTGAAGCAGAATATAACATCTTAAAA GCACTTTCTGACCACCCTAATGTGGTCAGATTCTATGGGATATACTTTAAGAAGGATAAAGTAAATGGAG ACAAGCTGTGGTTGGTTCTTGAGCTCTGCAGTGGAGGATCAGTGACTGACCTTGTGAAAGGATTTCTGAA GAGGGGTGAAAGAATGAGTGAGCCTCTAATTGCCTATATTTTACATGAAGCACTAATGGGACTTCAACAT TTGCATAACAACAAAACTATCCACAGAGATGTGAAAGGCAATAACATTCTATTGACCACGGAAGGTGGAG TGAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCACCAGTACCCGGCACCGTCGGAACACATCCGTAGG AACACCGTTTTGGATGGCTCCTGAGGTGATTGCATGTGAACAGCAATTGGATACCACTTATGACGCCAGA TGTGACACTTGGTCCCTGGGTATCACGGCCATTGAGCTGGGTGATGGAGATCCTCCACTAGCTGACCTTC ATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCAAAACTAAGGCAGCCTGAGCTATGGTC AGCAGAATTCAATGACTTCATAAGCAAGTGCTTGACTAAAGATTATGAAAAGCGTCCAACAGTGTCAGAA CTTTTACAGCATAAATTCATTACTCAAATTGAGGGCAAAGATGTGATGCTACAAAAACAACTAACGGAAT TCATTGGCATCCATCAATGCATGGGAGGCACAGAAAAGGCCAGACGTGAACGTATTCACACGAAGAAAGG GAACTTCAACCGACCTCTAATATCCAATCTGAAGGATGTAGATGATTTAGCAACCCTAGAAATTTTGGAT GAGAATACAGTCTCAGAGCAACTTGAGAAGTGTTATTCCAGAGATCAGATCTACGTCTATGTGGGAGACA TACTCATTGCTCTTAACCCTTTTCAGAGTCTGGGTCTTTACTCCACAAAGCATTCCAAACTATATATTGG ATCAAAGAGAACTGCCAGTCCTCCTCACATTTTTGCAATGGCTGACTTAGGATATCAATCTATAATAACA TATAATTCAGATCAGTGCATTGTTATTTCTGGAGAAAGTGGTGCTGGAAAGACTGAAAATGCTCATCTTT TAGTTCAGCAGCTGACAGTGCTTGGAAAGGCTAATAACAGAACCTTGCAAGAGAAGATTTTACAAGTGAA CAATTTGGTAGAAGCCTTTGGCAATGCCTGCACTATTATAAATGACAATTCTAGCAGATTTGGAAAATAC TTAGAAATGAAATTCACCTCTTCTGGAGCGGTAGTGGGAGCACAGATTTCTGAATATCTCCTGGAAAAAT CCCGAGTTATCCACCAAGCTATTGGAGAAAAAAATTTTCATATTTTTTACTACATTTATGCTGGTTTGGC TGAAAAGAAGAAACTAGCCCATTACAAACTGCCTGAAAATAAGCCTCCCAGGTACCTACAAAATGACCAC CTCAGAACAGTACAAGACATCATGAATAATAGTTTCTATAAATCCCAGTATGAATTAATTGAGCAATGTT TCAAAGTCATAGGTTTTACAATGGAGCAACTTGGTAGTATATACAGCATACTCGCTGCAATCTTGAATGT TGGCAACATTGAATTTTCTTCTGTGGCAACTGAACACCAGATTGACAAGAGCCACATTTCTAATCATACA GCCCTGGAGAACTGTGTTTCTTTGCTTTGCATTCGGGCAGATGAGCTACAAGAAGCTCTCACCTCCCACT GTGTGGTCACTAGAGGAGAAACAATTATACGACCCAATACTGTAGAAAAAGCTACCGATGTCAGGGATGC CATGGCTAAAACTTTATATGGACGTCTCTTTAGTTGGATAGTCAATTGCATTAACAGTTTGTTGAAGCAT GACTCATCACCAAGTGGGAATGGTGATGAGCTGAGCATTGGCATTCTTGATATATTTGGTTTTGAAAATT TCAAAAAAAATTCCTTCGAGCAGCTGTGCATTAACATTGCAAATGAACAAATTCAGTATTATTATAATCA ACATGTGTTTGCATGGGAACAGAATGAATACCTAAATGAAGATGTGGATGCTAGAGTTATTGAATATGAG GATAACTGGCCCCTCTTAGATATGTTTCTGCAAAAGCCAATGGGTTTACTTTCCCTACTTGATGAAGAAA GTAGATTTCCCAAGGCCACTGACCAGACTCTTGTAGAAAAATTTGAAGGTAACCTGAAATCACAATACTT CTGGAGACCCAAAAGAATGGAACTTAGTTTTGGAATTCACCATTATGCAGGAAAGGTCCTCTATAATGCA AGTGGATTCTTAGCCAAAAACAGAGACACTCTTCCTACTGACATTGTGCTACTTTTGAGGTCATCCGACA ACAGTGTAATTAGGCAACTAGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACATTCTAAAACTAA AAATGTTATAAACTATCAAATGAGGACTTCAGAAAAATTAATCAACCTGGCAAAGGGCGACACTGGAGAA GCCACACGTCATGCCAGAGAGACAACCAACATGAAAACACAAACGGTTGCATCATATTTTAGATATTCCC TGATGGATTTGTTGTCTAAAATGGTGGTGGGCCAACCTCATTTTGTCCGTTGCATCAAACCAAATAGTGA GCGTCAGGCAAGAAAATATGACAAAGAGAAAGTTCTGCTACAGCTTCGGTACACAGGAATTCTGGAAACA GCAAGAATTCGAAGACTAGGATTCTCCCATCGGATACTTTTTGCTAACTTTATAAAGCGGTACTACCTTC TCTGCTACAAGTCGAGCGAGGAGCCCCGCATGAGCCCTGACACCTGTGCCACCATTTTGGAAAAAGCTGG TCTCGATAACTGGGCTCTTGGAAAAACAAAAGTGTTCCTTAAGTATTATCACGTGGAGCAGTTAAATCTA ATGCGAAAGGAAGCTATTGACAAGCTTATTTTGATTCAAGCTTGTGTCAGAGCATTCTTGTGTTCAAGAA GATACCAAAAAATACAGGAGAAAAGGAAAGAAAGCGCTATAATAATACAGTCAGCTGCAAGAGGACACCT TGTCGGGAAACAAAGAAAAGAAATTGTTGACATGAAAAACACAGCAGTAACAACCATTCAAACTTCTGAT CAGGAATTCGACTACAAGAAAAACTTTGAAAATACAAGGGAATCTTTCGTGAAGAAACAAGCAGAAAATG CAATCTCTGCTAATGAAAGATTCATTTCAGCTCCAAATAATAAAGGAAGTGTATCTGTAGTGAAGACTTC CACTTTCAAACCTGAAGAGGAAACCACCAATGCTGTGGAGAGTAACAACAGAGTGTATCAGACTCCAAAA AAAATGAATAATGTGTATGAGGAAGAGGTTAAGCAAGAATTTTACCTTGTAGGGCCAGAAGTAAGCCCCA AACAGAAGTTTGTCAAAGACCTGGAAGAGAACAGCAATCTAAGGAAAGTGGAGAAAGAGGAAGCTATGAT CCAGAGTTACTATCAGAGGTACACAGAGGAGAGGAATTGTGAAGAGTCAAAAGCAGCATATCTAGAAAGG AAGGCCATATCAGAAAGGCCAAGCTACCCAGTGCCTTGGTTAGCTGAAAATGAGACTTCCTTTAAAAAAA CTTTGGAACCTACACTTAGCCAAAGGTCAATTTATCAAAATGCAAACAGCATGGAAAAAGAAAAGAAGAC ATCTGTAGTTACCCAGAGTGCACCGATATGCAGCCAGGAGGAAGGCAGAGGCCGTCTGAGGCATGAGACA GTCAAAGAGAGGCAAGTTGAACCAGTGACACAGGCCCAGGAGGAAGAAGATAAAGCAGCGGTATTCATTC AGAGCAAATACCGGGGTTACAAGAGAAGGCAGCAGTTGAGGAAGGACAAGATGTCTTCTTTTAAGCATCA GAAGATTGTCACAACACCAACAGAAGTAGCAAGAAACACTCATAATTTGTATTCCTATCCCACAAAACAT GAGGAAATCAATAACATCAAGAAGAAGGATAACAAAGACTCGAAAGCAACTTCAGAAAGAGAAGCATGTG GTTTGGCAATTTTTTCAAAACAGATATCAAAGTTATCTGAAGAATATTTCATTCTGCAGAAAAAATTGAA TGAAATGATTTTGTCACAGCAACTGAAGTCACTTTATCTGGGTGTCTCGCACCATAAGCCAATTAATAGA CGAGTTTCTTCTCAGCAGTGCCTCTCAGGTGTCTGTAAAGGAGAGGAGCCAAAAATATTGAGACCCCCAA GACGACCCCGGAAACCCAAAACATTAAATAACCCTGAAGACTCCACATACTATTATCTACTTCATAAGTC AATCCAAGAAGAAAAACGAAGACCAAGGAAAGACAGTCAGGGAAAATTATTAGATTTGGAAGATTTCTAT TATAAGGAATTTTTGCCCAGTCGTTCTGGACCAAAGGAACATAGCCCTAGTTTAAGAGAACGAAGACCAC AGCAAGAACTCCAGAATCAATGTATTAAGGCTAATGAAAGGTGCTGGGCGGCGGAGAGCCCCGAGAAGGA GGAGGAGAGAGAGCCAGCAGCCAACCCCTACGACTTCAGGAGGCTCCTGCGCAAAACCTCCCAGCGCCGG CGCCTCGTCCAGCAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215401 representing NM_017433
Red=Cloning site Green=Tags(s) MFPLIGKTIIFDNFPDPSDTWEITETIGKGTYGKVFKVLNKKNGQKAAVKILDPIHDIDEEIEAEYNILK ALSDHPNVVRFYGIYFKKDKVNGDKLWLVLELCSGGSVTDLVKGFLKRGERMSEPLIAYILHEALMGLQH LHNNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRHRRNTSVGTPFWMAPEVIACEQQLDTTYDAR CDTWSLGITAIELGDGDPPLADLHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSE LLQHKFITQIEGKDVMLQKQLTEFIGIHQCMGGTEKARRERIHTKKGNFNRPLISNLKDVDDLATLEILD ENTVSEQLEKCYSRDQIYVYVGDILIALNPFQSLGLYSTKHSKLYIGSKRTASPPHIFAMADLGYQSIIT YNSDQCIVISGESGAGKTENAHLLVQQLTVLGKANNRTLQEKILQVNNLVEAFGNACTIINDNSSRFGKY LEMKFTSSGAVVGAQISEYLLEKSRVIHQAIGEKNFHIFYYIYAGLAEKKKLAHYKLPENKPPRYLQNDH LRTVQDIMNNSFYKSQYELIEQCFKVIGFTMEQLGSIYSILAAILNVGNIEFSSVATEHQIDKSHISNHT ALENCVSLLCIRADELQEALTSHCVVTRGETIIRPNTVEKATDVRDAMAKTLYGRLFSWIVNCINSLLKH DSSPSGNGDELSIGILDIFGFENFKKNSFEQLCINIANEQIQYYYNQHVFAWEQNEYLNEDVDARVIEYE DNWPLLDMFLQKPMGLLSLLDEESRFPKATDQTLVEKFEGNLKSQYFWRPKRMELSFGIHHYAGKVLYNA SGFLAKNRDTLPTDIVLLLRSSDNSVIRQLVNHPLTKTGNLPHSKTKNVINYQMRTSEKLINLAKGDTGE ATRHARETTNMKTQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNSERQARKYDKEKVLLQLRYTGILET ARIRRLGFSHRILFANFIKRYYLLCYKSSEEPRMSPDTCATILEKAGLDNWALGKTKVFLKYYHVEQLNL MRKEAIDKLILIQACVRAFLCSRRYQKIQEKRKESAIIIQSAARGHLVGKQRKEIVDMKNTAVTTIQTSD QEFDYKKNFENTRESFVKKQAENAISANERFISAPNNKGSVSVVKTSTFKPEEETTNAVESNNRVYQTPK KMNNVYEEEVKQEFYLVGPEVSPKQKFVKDLEENSNLRKVEKEEAMIQSYYQRYTEERNCEESKAAYLER KAISERPSYPVPWLAENETSFKKTLEPTLSQRSIYQNANSMEKEKKTSVVTQSAPICSQEEGRGRLRHET VKERQVEPVTQAQEEEDKAAVFIQSKYRGYKRRQQLRKDKMSSFKHQKIVTTPTEVARNTHNLYSYPTKH EEINNIKKKDNKDSKATSEREACGLAIFSKQISKLSEEYFILQKKLNEMILSQQLKSLYLGVSHHKPINR RVSSQQCLSGVCKGEEPKILRPPRRPRKPKTLNNPEDSTYYYLLHKSIQEEKRRPRKDSQGKLLDLEDFY YKEFLPSRSGPKEHSPSLRERRPQQELQNQCIKANERCWAAESPEKEEEREPAANPYDFRRLLRKTSQRR RLVQQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017433 |
| ORF Size | 4848 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017433.5 |
| RefSeq Size | 5597 bp |
| RefSeq ORF | 4851 bp |
| Locus ID | 53904 |
| UniProt ID | Q8NEV4 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 186 kDa |
| Gene Summary | The protein encoded by this gene belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. The protein encoded by this gene plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of this gene is highly restricted, with the strongest expression in retina and cochlea. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215401L3 | Lenti-ORF clone of MYO3A (Myc-DDK-tagged)-Human myosin IIIA (MYO3A) |
CNY 18,050.00 |
|
| RC215401L4 | Lenti-ORF clone of MYO3A (mGFP-tagged)-Human myosin IIIA (MYO3A) |
CNY 18,050.00 |
|
| RG215401 | MYO3A (tGFP-tagged) - Human myosin IIIA (MYO3A) |
CNY 17,770.00 |
|
| SC304463 | MYO3A (untagged)-Human myosin IIIA (MYO3A) |
CNY 31,160.00 |
