SAMD4A (NM_015589) Human Tagged ORF Clone
CAT#: RC215243
SAMD4A (Myc-DDK-tagged)-Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1
ORF Plasmid: tGFP
"NM_015589" in other vectors (4)
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CNY 5,256.00
CNY 7,510.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SAMD4; SMAUG; SMAUG1; SMG; SMGA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215243 representing NM_015589
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGCGACCAGGTCGGGGTGCTGGCGGGCTGGTTTAAGGGCTGGAACGAGTGCGAGCAGACTGTTG CGCTGCTGTCGCTGCTCAAGCGCGTGAGCCAGACCCAGGCCCGCTTCCTCCAGCTCTGCCTGGAGCACTC GCTGGCCGACTGCGCCGAGCTGCACGTCCTCGAACGCGAGGCCAACAGCCCCGGAATCATTAACCAATGG CAACAGGAATCCAAGGATAAAGTGATTTCCCTCCTGTTAACTCATCTGCCTTTGCTGAAGCCAGGAAACC TCGACGCGAAAGTAGAATATATGAAACTGCTGCCCAAAATCCTGGCTCACTCTATTGAACACAACCAGCA CATTGAGGAGAGCAGGCAGCTGCTGTCCTATGCTTTGATACATCCAGCCACTTCGTTAGAAGACCGTAGT GCTTTAGCCATGTGGCTGAATCACTTGGAGGACCGCACGTCGACCAGCTTTGGTGGCCAGAACCGAGGCC GCTCAGACTCTGTGGATTATGGACAGACACACTACTATCACCAAAGACAGAACTCTGATGACAAGCTCAA TGGGTGGCAGAACTCTCGGGATTCTGGGATTTGCATCAATGCCTCCAACTGGCAGGACAAAAGCATGGGG TGTGAGAATGGCCATGTGCCCCTCTACTCCTCCTCATCTGTCCCCACCACAATCAATACGATTGGAACCA GCACAAGTACAATTCTCTCAGGCCAGGCACACCACAGCCCTTTGAAACGATCTGTGTCCCTTACCCCACC CATGAATGTGCCAAACCAGCCTCTAGGACATGGATGGATGTCTCATGAGGACTTACGAGCTAGAGGACCC CAGTGCCTCCCATCCGATCATGCCCCCCTGTCTCCACAAAGCAGTGTAGCCTCTTCAGGAAGTGGTGGGA GTGAACACTTAGAAGATCAGACCACTGCTCGTAACACATTCCAAGAAGAAGGTAGTGGGATGAAAGATGT TCCAGCCTGGCTGAAAAGCCTCCGCCTGCACAAATATGCCGCGCTTTTCTCCCAGATGACCTATGAGGAG ATGATGGCCCTCACCGAGTGCCAGCTGGAGGCGCAGAATGTTACCAAAGGTGCAAGACACAAAATTGTCA TCAGTATTCAGAAGCTCAAAGAAAGACAAAATCTCCTGAAGTCTTTGGAAAGGGACATCATCGAGGGGGG CAGCCTGCGCATCCCGCTCCAGGAACTGCACCAGATGATCCTGACTCCGATCAAGGCCTACAGCTCCCCG AGCACCACCCCCGAGGCTCGCCGCCGGGAGCCCCAGGCCCCGCGTCAGCCCTCACTGATGGGCCCCGAGA GCCAGAGCCCCGACTGCAAAGATGGGGCCGCAGCCACTGGCGCCACGGCCACCCCCTCGGCCGGGGCCAG CGGGGGGCTCCAGCCGCACCAGCTGAGCAGCTGCGATGGGGAGCTGGCCGTCGCCCCCCTGCCAGAGGGG GACCTCCCCGGGCAGTTCACACGCGTCATGGGGAAAGTGTGCACACAGCTCTTGGTCTCCAGACCTGATG AGGAAAATATAAGTTCCTATTTACAGCTCATAGACAAGTGTCTAATTCATGAGGCATTTACAGAGACACA GAAAAAAAGATTGTTGTCATGGAAACAGCAGGTGCAGAAGCTCTTTCGGTCTTTCCCTCGGAAAACCCTT CTAGACATATCAGGATATCGACAGCAAAGAAATCGAGGCTTTGGGCAATCCAACTCCCTCCCGACGGCTG GCTCTGTGGGCGGTGGCATGGGCAGACGGAACCCGCGCCAGTACCAGATCCCCTCTCGGAACGTCCCTTC CGCCCGCCTGGGCCTCTTGGGCACCAGTGGATTCGTCAGCTCCAACCAGCGCAACACCACAGCTACCCCC ACCATCATGAAACAAGGAAGACAGAACCTGTGGTTTGCCAACCCCGGGGGCAGCAATAGCATGCCAAGCC GCACCCACAGCTCAGTCCAGAGGACCCGCTCGCTGCCCGTGCACACTTCCCCACAGAACATGCTGATGTT CCAGCAGCCAGAATTCCAGCTTCCCGTGACCGAACCTGACATCAACAACAGGCTGGAGTCGTTGTGCCTC AGTATGACCGAACACGCCCTGGGAGACGGGGTTGACCGGACCTCCACCATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC215243 representing NM_015589
Red=Cloning site Green=Tags(s) MFRDQVGVLAGWFKGWNECEQTVALLSLLKRVSQTQARFLQLCLEHSLADCAELHVLEREANSPGIINQW QQESKDKVISLLLTHLPLLKPGNLDAKVEYMKLLPKILAHSIEHNQHIEESRQLLSYALIHPATSLEDRS ALAMWLNHLEDRTSTSFGGQNRGRSDSVDYGQTHYYHQRQNSDDKLNGWQNSRDSGICINASNWQDKSMG CENGHVPLYSSSSVPTTINTIGTSTSTILSGQAHHSPLKRSVSLTPPMNVPNQPLGHGWMSHEDLRARGP QCLPSDHAPLSPQSSVASSGSGGSEHLEDQTTARNTFQEEGSGMKDVPAWLKSLRLHKYAALFSQMTYEE MMALTECQLEAQNVTKGARHKIVISIQKLKERQNLLKSLERDIIEGGSLRIPLQELHQMILTPIKAYSSP STTPEARRREPQAPRQPSLMGPESQSPDCKDGAAATGATATPSAGASGGLQPHQLSSCDGELAVAPLPEG DLPGQFTRVMGKVCTQLLVSRPDEENISSYLQLIDKCLIHEAFTETQKKRLLSWKQQVQKLFRSFPRKTL LDISGYRQQRNRGFGQSNSLPTAGSVGGGMGRRNPRQYQIPSRNVPSARLGLLGTSGFVSSNQRNTTATP TIMKQGRQNLWFANPGGSNSMPSRTHSSVQRTRSLPVHTSPQNMLMFQQPEFQLPVTEPDINNRLESLCL SMTEHALGDGVDRTSTI SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015589 |
ORF Size | 2151 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015589.4 |
RefSeq Size | 2356 bp |
RefSeq ORF | 2157 bp |
Locus ID | 23034 |
UniProt ID | Q9UPU9 |
MW | 79.1 kDa |
Gene Summary | Sterile alpha motifs (SAMs) in proteins such as SAMD4A are part of an RNA-binding domain that functions as a posttranscriptional regulator by binding to an RNA sequence motif known as the Smaug recognition element, which was named after the Drosophila Smaug protein (Baez and Boccaccio, 2005 [PubMed 16221671]).[supplied by OMIM, Mar 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
,Amadei, G;Zander, MA;Yang, G;Dumelie, JG;Vessey, JP;Lipshitz, HD;Smibert, CA;Kaplan, DR;Miller, FD;,
J. Neurosci.
,PubMed ID 26609159
[SAMD4A]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215243L3 | Lenti ORF clone of Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1, Myc-DDK-tagged |
CNY 7,656.00 |
|
RC215243L4 | Lenti ORF clone of Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1, mGFP tagged |
CNY 9,410.00 |
|
RG215243 | SAMD4A (tGFP-tagged) - Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1 |
CNY 8,270.00 |
|
SC304332 | SAMD4A (untagged)-Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1 |
CNY 13,780.00 |