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SAFB (NM_002967) Human Tagged ORF Clone
CAT＃: RC215199
- TrueORF®
SAFB (Myc-DDK-tagged)-Human scaffold attachment factor B (SAFB), transcript variant 3. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_002967" in other vectors (4)
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CNY 10,048.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215199 representing NM_002967, codon optimized.
Due to the complexity of NM_002967, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGAAACCCTGTCTGGACTCGGCGACAGCGGCGCAGCCGGAGCTGCCGCCCTGAGCAGCGCCAGT TCAGAGACAGGTACCAGAAGGCTCAGTGATTTGCGGGTCATAGATTTGCGGGCAGAATTGAGAAAAAGA AACGTCGACAGTTCTGGGAACAAGTCAGTGCTTATGGAGCGACTCAAAAAAGCCATCGAGGACGAGGGC GGGAATCCCGACGAGATCGAGATAACGTCTGAGGGGAACAAAAAAACAAGCAAGAGGTCTAGCAAGGGC CGCAAGCCAGAGGAAGAAGGCGTCGAAGATAATGGACTGGAGGAGAACAGTGGTGACGGGCAGGAAGAT GTCGAGACTTCTCTTGAAAACCTGCAAGATATCGATATTATGGACATTTCCGTCTTGGACGAAGCGGAG ATCGACAACGGTAGTGTGGCCGATTGCGTGGAGGACGATGACGCCGATAACCTGCAGGAGAGTCTGTCT GATTCACGCGAGTTGGTTGAGGGCGAGATGAAGGAGCTGCCCGAACAGCTTCAAGAACACGCCATTGAG GACAAAGAAACCATCAATAACCTTGACACGAGCAGCAGTGATTTTACCATCTTGCAAGAAATAGAAGAG CCTTCTCTGGAACCCGAAAACGAGAAAATTCTGGACATCCTGGGGGAGACTTGCAAATCAGAGCCCGTG AAAGAAGAATCCTCTGAACTGGAACAACCTTTCGCACAGGACACTAGCTCTGTGGGCCCCGATCGAAAA TTGGCTGAAGAGGAGGACCTTTTCGACTCAGCCCACCCCGAGGAGGGTGACCTGGATCTGGCCAGCGAA TCAACAGCACATGCGCAATCATCCAAAGCAGATTCACTGTTGGCCGTAGTGAAACGAGAGCCAGCCGAG CAGCCAGGGGACGGAGAAAGAACCGACTGCGAGCCTGTGGGCCTTGAGCCAGCAGTGGAACAGAGCAGT GCAGCATCAGAATTGGCGGAAGCATCAAGTGAAGAGCTGGCCGAAGCCCCCACAGAGGCACCTAGCCCT GAAGCTCGCGACTCTAAAGAGGACGGCAGGAAATTTGATTTTGACGCATGCAACGAGGTGCCACCAGCG CCAAAGGAGAGCAGTACATCAGAAGGCGCCGACCAGAAAATGTCTAGCCCTGAGGACGACTCCGATACA AAGAGGTTGAGCAAAGAGGAGAAAGGCCGAAGCAGTTGCGGACGCAACTTTTGGGTGTCTGGCCTTTCC TCCACCACAAGGGCTACCGACCTGAAGAACCTCTTCTCAAAGTACGGAAAGGTGGTGGGCGCAAAAGTG GTTACTAATGCACGCAGCCCTGGTGCCCGCTGCTATGGATTTGTAACTATGAGCACGGCTGAAGAAGCT ACAAAGTGTATCAATCACCTCCACAAGACTGAACTGCATGGAAAAATGATCAGTGTCGAGAAGGCCAAA AACGAACCAGTGGGGAAAAAAACATCTGACAAGAGGGACAGCGATGGTAAGAAGGAAAAAAGCAGCAAT TCTGATCGCAGTACAAATCTTAAACGAGATGACAAATGTGACCGGAAAGACGACGCCAAGAAAGGCGAC GATGGGTCCGGGGAAAAATCTAAGGATCAGGATGACCAAAAGCCGGGACCATCAGAAAGGAGCCGCGCT ACGAAAAGCGGGAGCCGGGGCACCGAACGCACGGTTGTAATGGACAAGAGTAAAGGCGTTCCTGTTATA AGCGTCAAGACGAGCGGATCTAAGGAGAGGGCCAGTAAGTCTCAAGACCGGAAGTCAGCTTCTAGAGAA AAGAGAAGCGTTGTGAGCTTTGATAAGGTTAAGGAGCCAAGGAAGAGCAGGGATTCAGAGTCTCACAGC CGGGTCCGGGAACGCTCAGAGCGCGAGCAGCGCATGCAGGCCCAGTGGGAGAGAGAAGAGCGGGAAAGG CTTGAGATTGCCAGGGAAAGGTTGGCCTTTCAGCGCCAGCGACTTGAGAGGGAGCGCATGGAGCGGGAG AGGCTTGAGCGAGAACGCATGCACGTCGAGCATGAAAGGAGACGGGAACAAGAGCGGATACACCGAGAA CGCGAGGAGCTCAGACGACAGCAAGAGCTTAGGTATGAGCAGGAACGAAGACCTGCAGTCCGGCGGCCT TACGACCTTGATCGGCGCGACGACGCATATTGGCCAGAGGCTAAAAGGGCTGCATTGGACGAGAGATAC CACTCAGATTTCAATAGGCAGGACAGATTTCACGACTTCGACCACCGGGATAGGGGGAGATATCCGGAC CACTCCGTGGACCGGCGCGAAGGGTCCCGGAGCATGATGGGAGAGCGCGAGGGGCAACATTATCCAGAG CGACACGGCGGCCCTGAACGGCATGGACGCGACTCTCGGGACGGATGGGGGGGGTACGGGTCCGATAAG AGAATGTCAGAAGGGCGGGGTCTTCCCCCACCTCCTAGGCGGGATTGGGGCGATCACGGCCGACGGGAA GACGACCGATCCTGGCAGGGAACTGCTGACGGCGGAATGATGGACAGGGACCACAAGCGGTGGCAGGGT GGTGAGCGATCAATGAGCGGACACTCCGGCCCTGGCCACATGATGAATCGCGGCGGCATGTCTGGTCGC GGGAGTTTTGCACCTGGCGGCGCCTCCAGGGGTCATCCGATTCCCCATGGGGGCATGCAGGGTGGCTTT GGTGGCCAAAGCCGCGGATCCCGCCCTAGCGACGCCAGGTTTACCCGCCGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC215199
Blue=ORF Red=Cloning site Green=Tag(s) MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEG GNPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAE IDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEE PSLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASE STAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSP EARDSKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLS STTRATDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAK NEPVGKKTSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRA TKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHS RVRERSEREQRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHRE REELRRQQELRYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPD HSVDRREGSRSMMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRRDWGDHGRRE DDRSWQGTADGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGF GGQSRGSRPSDARFTRRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002967 |
| ORF Size | 2745 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002967.1, NM_002967.2, NM_002967.3, NP_002958.2 |
| RefSeq Size | 3115 bp |
| RefSeq ORF | 2748 bp |
| Locus ID | 6294 |
| UniProt ID | Q15424 |
| Domains | RRM, SAP |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 102.6 kDa |
| Gene Summary | This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215199L3 | Lenti-ORF clone of SAFB (Myc-DDK-tagged)-Human scaffold attachment factor B (SAFB), transcript variant 3. Note: ORF is codon optimized |
CNY 9,030.00 |
|
| RC215199L4 | Lenti-ORF clone of SAFB (mGFP-tagged)-Human scaffold attachment factor B (SAFB), transcript variant 3. Note: ORF is codon optimized |
CNY 12,448.00 |
|
| RG215199 | SAFB (tGFP-tagged) - Human scaffold attachment factor B (SAFB), transcript variant 3 |
CNY 11,648.00 |
|
| SC118304 | SAFB (untagged)-Human scaffold attachment factor B (SAFB), transcript variant 3 |
CNY 6,712.00 |
