CD130 (IL6ST) (NM_002184) Human Tagged ORF Clone
CAT#: RC215123
IL6ST (Myc-DDK-tagged)-Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1
ORF Plasmid: tGFP
"NM_002184" in other vectors (6)
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CNY 6,720.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CD130; CDW130; GP130; HIES4; IL-6RB; sGP130 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215123 representing NM_002184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGACGTTGCAGACTTGGCTAGTGCAAGCCTTGTTTATTTTCCTCACCACTGAATCTACAGGTGAAC TTCTAGATCCATGTGGTTATATCAGTCCTGAATCTCCAGTTGTACAACTTCATTCTAATTTCACTGCAGT TTGTGTGCTAAAGGAAAAATGTATGGATTATTTTCATGTAAATGCTAATTACATTGTCTGGAAAACAAAC CATTTTACTATTCCTAAGGAGCAATATACTATCATAAACAGAACAGCATCCAGTGTCACCTTTACAGATA TAGCTTCATTAAATATTCAGCTCACTTGCAACATTCTTACATTCGGACAGCTTGAACAGAATGTTTATGG AATCACAATAATTTCAGGCTTGCCTCCAGAAAAACCTAAAAATTTGAGTTGCATTGTGAACGAGGGGAAG AAAATGAGGTGTGAGTGGGATGGTGGAAGGGAAACACACTTGGAGACAAACTTCACTTTAAAATCTGAAT GGGCAACACACAAGTTTGCTGATTGCAAAGCAAAACGTGACACCCCCACCTCATGCACTGTTGATTATTC TACTGTGTATTTTGTCAACATTGAAGTCTGGGTAGAAGCAGAGAATGCCCTTGGGAAGGTTACATCAGAT CATATCAATTTTGATCCTGTATATAAAGTGAAGCCCAATCCGCCACATAATTTATCAGTGATCAACTCAG AGGAACTGTCTAGTATCTTAAAATTGACATGGACCAACCCAAGTATTAAGAGTGTTATAATACTAAAATA TAACATTCAATATAGGACCAAAGATGCCTCAACTTGGAGCCAGATTCCTCCTGAAGACACAGCATCCACC CGATCTTCATTCACTGTCCAAGACCTTAAACCTTTTACAGAATATGTGTTTAGGATTCGCTGTATGAAGG AAGATGGTAAGGGATACTGGAGTGACTGGAGTGAAGAAGCAAGTGGGATCACCTATGAAGATAGACCATC TAAAGCACCAAGTTTCTGGTATAAAATAGATCCATCCCATACTCAAGGCTACAGAACTGTACAACTCGTG TGGAAGACATTGCCTCCTTTTGAAGCCAATGGAAAAATCTTGGATTATGAAGTGACTCTCACAAGATGGA AATCACATTTACAAAATTACACAGTTAATGCCACAAAACTGACAGTAAATCTCACAAATGATCGCTATCT AGCAACCCTAACAGTAAGAAATCTTGTTGGCAAATCAGATGCAGCTGTTTTAACTATCCCTGCCTGTGAC TTTCAAGCTACTCACCCTGTAATGGATCTTAAAGCATTCCCCAAAGATAACATGCTTTGGGTGGAATGGA CTACTCCAAGGGAATCTGTAAAGAAATATATACTTGAGTGGTGTGTGTTATCAGATAAAGCACCCTGTAT CACAGACTGGCAACAAGAAGATGGTACCGTGCATCGCACCTATTTAAGAGGGAACTTAGCAGAGAGCAAA TGCTATTTGATAACAGTTACTCCAGTATATGCTGATGGACCAGGAAGCCCTGAATCCATAAAGGCATACC TTAAACAAGCTCCACCTTCCAAAGGACCTACTGTTCGGACAAAAAAAGTAGGGAAAAACGAAGCTGTCTT AGAGTGGGACCAACTTCCTGTTGATGTTCAGAATGGATTTATCAGAAATTATACTATATTTTATAGAACC ATCATTGGAAATGAAACTGCTGTGAATGTGGATTCTTCCCACACAGAATATACATTGTCCTCTTTGACTA GTGACACATTGTACATGGTACGAATGGCAGCATACACAGATGAAGGTGGGAAGGATGGTCCAGAATTCAC TTTTACTACCCCAAAGTTTGCTCAAGGAGAAATTGAAGCCATAGTCGTGCCTGTTTGCTTAGCATTCCTA TTGACAACTCTTCTGGGAGTGCTGTTCTGCTTTAATAAGCGAGACCTAATTAAAAAACACATCTGGCCTA ATGTTCCAGATCCTTCAAAGAGTCATATTGCCCAGTGGTCACCTCACACTCCTCCAAGGCACAATTTTAA TTCAAAAGATCAAATGTATTCAGATGGCAATTTCACTGATGTAAGTGTTGTGGAAATAGAAGCAAATGAC AAAAAGCCTTTTCCAGAAGATCTGAAATCATTGGACCTGTTCAAAAAGGAAAAAATTAATACTGAAGGAC ACAGCAGTGGTATTGGGGGGTCTTCATGCATGTCATCTTCTAGGCCAAGCATTTCTAGCAGTGATGAAAA TGAATCTTCACAAAACACTTCGAGCACTGTCCAGTATTCTACCGTGGTACACAGTGGCTACAGACACCAA GTTCCGTCAGTCCAAGTCTTCTCAAGATCCGAGTCTACCCAGCCCTTGTTAGATTCAGAGGAGCGGCCAG AAGATCTACAATTAGTAGATCATGTAGATGGCGGTGATGGTATTTTGCCCAGGCAACAGTACTTCAAACA GAACTGCAGTCAGCATGAATCCAGTCCAGATATTTCACATTTTGAAAGGTCAAAGCAAGTTTCATCAGTC AATGAGGAAGATTTTGTTAGACTTAAACAGCAGATTTCAGATCATATTTCACAATCCTGTGGATCTGGGC AAATGAAAATGTTTCAGGAAGTTTCTGCAGCAGATGCTTTTGGTCCAGGTACTGAGGGACAAGTAGAAAG ATTTGAAACAGTTGGCATGGAGGCTGCGACTGATGAAGGCATGCCTAAAAGTTACTTACCACAGACTGTA CGGCAAGGCGGCTACATGCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215123 representing NM_002184
Red=Cloning site Green=Tags(s) MLTLQTWLVQALFIFLTTESTGELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTN HFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGK KMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSD HINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTAST RSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLV WKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACD FQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESK CYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRT IIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEAIVVPVCLAFL LTTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDGNFTDVSVVEIEAND KKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSDENESSQNTSSTVQYSTVVHSGYRHQ VPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSV NEEDFVRLKQQISDHISQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTV RQGGYMPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002184 |
ORF Size | 2754 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002184.4 |
RefSeq Size | 3242 bp |
RefSeq ORF | 2757 bp |
Locus ID | 3572 |
UniProt ID | P40189 |
Domains | FN3 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
MW | 103.54 kDa |
Gene Summary | The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma
,null,
Cells
,PubMed ID 36359879
[CD130]
|
Dominant-negative mutations in human IL6ST underlie hyper-IgE syndrome
,Béziat, V;Tavernier, SJ;Chen, YH;Ma, CS;Materna, M;Laurence, A;Staal, J;Aschenbrenner, D;Roels, L;Worley, L;Claes, K;Gartner, L;Kohn, LA;De Bruyne, M;Schmitz-Abe, K;Charbonnier, LM;Keles, S;Nammour, J;Vladikine, N;Maglorius Renkilaraj, MRL;Seeleuthner, Y;Migaud, M;Rosain, J;Jeljeli, M;Boisson, B;Van Braeckel, E;Rosenfeld, JA;Dai, H;Burrage, LC;Murdock, DR;Lambrecht, BN;Avettand-Fenoel, V;Vogel, TP;, ;Esther, CR;Haskologlu, S;Dogu, F;Ciznar, P;Boutboul, D;Ouachée-Chardin, M;Amourette, J;Lebras, MN;Gauvain, C;Tcherakian, C;Ikinciogullari, A;Beyaert, R;Abel, L;Milner, JD;Grimbacher, B;Couderc, LJ;Butte, MJ;Freeman, AF;Catherinot, É;Fieschi, C;Chatila, TA;Tangye, SG;Uhlig, HH;Haerynck, F;Casanova, JL;Puel, A;,
J. Exp. Med.
,PubMed ID 32207811
[CD130]
|
Endogenous transmembrane protein UT2 inhibits pSTAT3 and suppresses hematological malignancy
,Lee, D;Wang, YH;Kalaitzidis, D;Ramachandran, J;Eda, H;Sykes, DB;Raje, N;Scadden, DT;,
J. Clin. Invest.
,PubMed ID 26927669
[CD130]
|
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215123L1 | Lenti ORF clone of Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1, Myc-DDK-tagged |
CNY 9,120.00 |
|
RC215123L2 | Lenti ORF clone of Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1, mGFP tagged |
CNY 9,120.00 |
|
RC215123L3 | Lenti ORF clone of Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215123L4 | Lenti ORF clone of Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1, mGFP tagged |
CNY 9,120.00 |
|
RG215123 | IL6ST (tGFP-tagged) - Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1 |
CNY 8,320.00 |
|
SC111643 | IL6ST (untagged)-Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1 |
CNY 6,728.00 |