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ANKLE1 (NM_152363) Human Tagged ORF Clone
CAT＃: RC214643
ANKLE1 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 1 (ANKLE1)
ORF Plasmid: tGFP
"NM_152363" in other vectors (6)
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CNY 6,864.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANKRD41; LEM3; LEMD6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214643 representing NM_152363
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTCGGAGGCCCGCCTGGCTCGCAGGTTGCGGGATGCGCTGCGGGAGGAGGAGCCGTGGGCAGTAG AGGAGCTGCTGCGCTGCGGCGCGGACCCTAATTTGGTGCTAGAGGACGGCGCAGCGGCTGTGCACTTGGC GGCCGGAGCCCGGCACCCGCGCGGCCTGCGTTGCCTCGGGGCCCTACTGCGCCAAGGCGGGGACCCCAAC GCTCGATCTGTCGAGGCACTGACGCCGCTGCATGTGGCCGCCGCGTGGGGCTGCCGCCGCGGCCTGGAGC TGCTGCTGAGCCAAGGAGCGGACCCGGCGCTGCGCGACCAGGACGGACTCCGGCCGCTGGACCTGGCCCT GCAGCAGGGACACCTGGAGTGCGCGCGAGTCCTGCAGGATCTCGACACGCGGACCAGGACCCGGACCCGG ATCGGGGCAGAGACTCAGGAGCCCGAGCCTGCACCTGGCACCCCAGGCCTCTCTGGACCTACCGATGAGA CGCTGGACTCCATAGCACTCCAAAAGCAGCCATGCAGAGGTGACAACAGGGACATTGGCTTGGAGGCTGA CCCAGGACCCCCCAGCCTCCCTGTTCCCCTTGAAACTGTGGACAAACATGGGAGCTCGGCGTCCCCTCCA GGGCACTGGGATTACAGCTCAGACGCCTCTTTCGTCACAGCGGTTGAGGTCTCTGGAGCTGAGGACCCAG CCTCGGACACTCCCCCCTGGGCTGGGTCATTGCCACCGACCAGGCAGGGACTTCTGCATGTTGTCCATGC CAACCAGAGGGTACCTAGGTCTCAGGGCACGGAGGCAGAACTGAATGCCCGTCTGCAGGCCCTGACTCTG ACCCCACCAAATGCTGCTGGCTTCCAGTCCTCCCCTTCCTCCATGCCTCTCCTGGACAGGAGTCCAGCTC ATAGCCCCCCACGGACACCAACCCCTGGAGCTTCTGACTGCCACTGCCTGTGGGAGCACCAGACATCCAT TGATAGTGACATGGCCACGCTCTGGCTGACAGAGGATGAGGCAAGCTCTACAGGTGGCAGGGAACCTGTC GGCCCTTGCCGGCACCTGCCAGTCTCCACTGTGTCTGACTTGGAGTTGCTGAAGGGACTCCGAGCACTTG GTGAGAATCCTCACCCCATCACACCCTTCACCAGGCAGTTGTACCACCAGCAGCTGGAAGAAGCCCAGAT TGCTCCTGGCCCAGAGTTTTCAGGGCACAGCCTAGAACTGGCTGCAGCCCTGCGGACGGGCTGTATTCCA GATGTCCAGGCAGATGAAGACGCGCTGGCCCAGCAGTTTGAGCAGCCAGATCCTGCCAGGAGGTGGCGGG AGGGGGTCGTGAAGTCTAGCTTCACCTATCTGCTGCTGGACCCCAGGGAGACTCAGGACCTGCCAGCCCG AGCCTTCTCACTGACCCCAGCTGAGCGCCTTCAGACTTTCATCCGTGCCATCTTCTACGTGGGCAAAGGG ACGAGGGCCCGGCCATATGTCCACCTCTGGGAGGCCCTTGGTCACCATGGGCGGTCAAGAAAACAGCCCC ACCAGGCCTGCCCCAAGGTGCGTCAGATCTTGGACATCTGGGCCAGTGGTTGCGGTGTTGTGTCCCTACA TTGCTTCCAGCACGTGGTCGCTGTGGAGGCTTATACACGGGAGGCGTGTATTGTGGAAGCCCTAGGGATC CAGACGCTCACCAACCAGAAGCAAGGGCACTGCTATGGAGTGGTGGCAGGCTGGCCACCTGCTCGTCGCC GGCGCTTGGGGGTGCACCTGCTGCACCGTGCCCTCCTTGTCTTCCTGGCTGAAGGCGAGCGACAGCTTCA TCCCCAGGACATCCAGGCCCGGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214643 representing NM_152363
Red=Cloning site Green=Tags(s) MCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPN ARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTR IGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPP GHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTL TPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPV GPCRHLPVSTVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSLELAAALRTGCIP DVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKG TRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGI QTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152363 |
| ORF Size | 1845 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152363.6 |
| RefSeq Size | 3217 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 126549 |
| UniProt ID | Q8NAG6 |
| MW | 66.7 kDa |
| Gene Summary | Endonuclease that probably plays a role in the DNA damage response and DNA repair.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214643L1 | Lenti ORF clone of Human ankyrin repeat and LEM domain containing 1 (ANKLE1), Myc-DDK-tagged |
CNY 9,264.00 |
|
| RC214643L2 | Lenti ORF clone of Human ankyrin repeat and LEM domain containing 1 (ANKLE1), mGFP tagged |
CNY 6,460.00 |
|
| RC214643L3 | Lenti ORF clone of Human ankyrin repeat and LEM domain containing 1 (ANKLE1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214643L4 | Lenti ORF clone of Human ankyrin repeat and LEM domain containing 1 (ANKLE1), mGFP tagged |
CNY 6,460.00 |
|
| RG214643 | ANKLE1 (tGFP-tagged) - Human ankyrin repeat and LEM domain containing 1 (ANKLE1) |
CNY 5,040.00 |
|
| SC318056 | ANKLE1 (untagged)-Human ankyrin repeat and LEM domain containing 1 (ANKLE1) |
CNY 9,880.00 |
