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Calpain 12 (CAPN12) (NM_144691) Human Tagged ORF Clone
CAT＃: RC214563
CAPN12 (Myc-DDK-tagged)-Human calpain 12 (CAPN12)
ORF Plasmid: tGFP
"NM_144691" in other vectors (4)
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CNY 5,264.00
CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214563 representing NM_144691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGAGCCGGGCGCC TGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTGGATTCGGGGATCCTGTTCCG CGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTATGACCAGCTGGGGCCGGACTCGGAGAAGGCC AAAGGCGTGAAATGGATGAGGCCCCATGAGTTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCC GCACAGACGTGTGTCAGGGGAGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTA TCCCCGGCTCCTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTGCGTGAGGGGA AGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTCCTGGAGAAGGCCTACGCCAA GCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATGAATGAGGCTTTTGTGGATTTCACAGGCGGC GTGGGCGAGGTGCTCTATCTGAGACAAAACAGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCA AGGAGTCCCTCGTGGGCGCCACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAA GGGACACGCGTATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGCTGGGACACAC TCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAGTTCTGGATGGAGCTGCGGGA CTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTGAGCCCGGAGGTGCTGGGCCCCAGCCCGGAG GGGGGCGGCTGGCACGTCCACACCTTCCAAGGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGC CTAATGCTGAAACCTTCTGGACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGA CGAGGATGAGGAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCCAAGGGCCTCA CTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTGGGCCTCTGGGATTCCCCGCG CAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGCTCGCCCCTCAGCGCCCGCCGCGACGTGACC CGCCGCTGCTGCCTGCGTCCAGGCCACTACCTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTG ACTTCACTCTGCGTGTCTTCTCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGA CCTGCAGTCTCTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAGCCTGCCAGGG CCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTGCTGCAGTGTTTCGGGCATGG GCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGCTACCTCCTGGAGTGGCAGGCCATATTTAAC AAGTTCGATGAGGACACCTCTGGAACCATGAACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCT TCCACCTGAACAACCAGCTGACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTT CGAGCGGTTCGTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC214563 representing NM_144691
Red=Cloning site Green=Tags(s) MASSSGRVTIQLVDEEAGVGAGRLQLFRGQSYEAIRAACLDSGILFRDPYFPAGPDALGYDQLGPDSEKA KGVKWMRPHEFCAEPKFICEDMSRTDVCQGSLGNCWFLAAAASLTLYPRLLRRVVPPGQDFQHGYAGVFH FQLWQFGRWMDVVVDDRLPVREGKLMFVRSEQRNEFWAPLLEKAYAKLHGSYEVMRGGHMNEAFVDFTGG VGEVLYLRQNSMGLFSALRHALAKESLVGATALSDRGEYRTEEGLVKGHAYSITGTHKVFLGFTKVRLLR LRNPWGCVEWTGAWSDSCPRWDTLPTECRDALLVKKEDGEFWMELRDFLLHFDTVQICSLSPEVLGPSPE GGGWHVHTFQGRWVRGFNSGGSQPNAETFWTNPQFRLTLLEPDEEDDEDEEGPWGGWGAAGARGPARGGR TPKCTVLLSLIQRNRRRLRAKGLTYLTVGFHVFQIPEELLGLWDSPRSHALLPRLLRADRSPLSARRDVT RRCCLRPGHYLVVPSTAHAGDEADFTLRVFSERRHTAVEIDDVISADLQSLQGPYLPLELGLEQLFQELA GEEEELNASQLQALLSIALEPARAHTSTPREIGLRTCEQLLQCFGHGQSLALHHFQQLWGYLLEWQAIFN KFDEDTSGTMNSYELRLALNAAGFHLNNQLTQTLTSRYRDSRLRVDFERFVSCVAHLTCIFCHCSQHLDG GEGVICLTHRQWMEVATFS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144691 |
| ORF Size | 2157 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144691.3, NP_653292.2 |
| RefSeq Size | 3129 bp |
| RefSeq ORF | 2160 bp |
| Locus ID | 147968 |
| UniProt ID | Q6ZSI9 |
| Protein Families | Druggable Genome |
| MW | 80.9 kDa |
| Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes a member of the calpain large subunit family. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214563L3 | Lenti ORF clone of Human calpain 12 (CAPN12), Myc-DDK-tagged |
CNY 9,500.00 |
|
| RC214563L4 | Lenti ORF clone of Human calpain 12 (CAPN12), mGFP tagged |
CNY 9,500.00 |
|
| RG214563 | CAPN12 (tGFP-tagged) - Human calpain 12 (CAPN12) |
CNY 8,360.00 |
|
| SC306183 | CAPN12 (untagged)-Human calpain 12 (CAPN12) |
CNY 13,870.00 |
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