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PSAPL1 (NM_001085382) Human Tagged ORF Clone
CAT＃: RC214503
- TrueORF®
PSAPL1 (Myc-DDK-tagged)-Human prosaposin-like 1 (gene/pseudogene) (PSAPL1)
ORF Plasmid: tGFP
"NM_001085382" in other vectors (4)
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CNY 5,816.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214503 representing NM_001085382
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGTGCCCTGCTCCTCCTGCCCAGCCTCCTGGGGGCCACCAGGGCCAGCCCCACCTCAGGCCCCC AGGAGTGTGCAAAGGGCTCCACGGTGTGGTGTCAGGATCTGCAGACAGCTGCCAGGTGCGGGGCTGTGGG GTACTGCCAAGGGGCCGTATGGAACAAACCCACCGCGAAGTCTCTGCCCTGCGACGTATGCCAGGACATA GCAGCCGCCGCTGGCAATGGGCTGAACCCTGACGCCACGGAGTCTGACATCCTGGCTTTGGTGATGAAGA CCTGTGAGTGGCTCCCCAGCCAGGAGTCTTCAGCCGGATGCAAGTGGATGGTGGATGCCCACAGTTCGGC CATCCTGAGCATGCTCCGTGGGGCCCCGGACAGTGCCCCGGCACAGGTGTGCACAGCGCTCAGCCTCTGT GAGCCGCTGCAGAGGCACCTGGCCACCCTGAGGCCACTCTCCAAAGAGGACACCTTTGAGGCTGTGGCTC CGTTCATGGCCAATGGGCCCCTTACCTTCCACCCCCGCCAGGCGCCTGAAGGAGCTCTGTGCCAAGACTG TGTACGGCAGGTCTCCCGACTCCAGGAGGCTGTCCGGTCCAACTTGACCTTGGCCGACTTGAACATCCAG GAGCAGTGTGAGTCCTTGGGGCCTGGCCTGGCCGTCCTCTGCAAGAACTACCTCTTCCAGTTTTTTGTCC CTGCTGACCAAGCACTGAGGCTTCTCCCCCCGCAGGAGCTCTGCAGGAAGGGGGGATTCTGTGAGGAGCT AGGGGCACCTGCCCGTTTGACTCAAGTAGTGGCCATGGACGGGGTCCCCTCCCTGGAGCTGGGGTTGCCA AGGAAACAGAGCGAGATGCAGATGAAGGCCGGTGTGACCTGTGAGGTGTGCATGAACGTGGTGCAGAAGC TGGACCACTGGCTCATGTCCAACAGCTCTGAGCTCATGATCACCCATGCCCTGGAGCGCGTGTGCTCGGT AATGCCTGCCTCTATCACGAAGGAGTGCATCATCTTGGTGGACACCTACAGCCCCTCCTTGGTGCAGCTT GTGGCCAAAATCACCCCAGAGAAGGTGTGCAAGTTCATCCGTCTGTGTGGCAACCGGAGGCGGGCCCGGG CAGTCCATGATGCCTATGCCATCGTGCCGTCCCCAGAGTGGGACGCGGAGAACCAGGGCAGCTTCTGCAA TGGGTGCAAGAGGCTGCTCACGGTGTCCTCCCACAACTTGGAGAGCAAGAGCACCAAGCGAGACATCCTG GTGGCCTTCAAGGGTGGCTGCAGCATCCTGCCGCTGCCCTATATGATCCAGTGCAAGCACTTCGTCACCC AGTACGAGCCCGTGCTCATTGAGAGTCTCAAGGACATGATGGACCCCGTGGCTGTGTGCAAGAAGGTGGG GGCCTGCCACGGCCCCAGGACCCCACTGCTGGGCACCGACCAGTGTGCCCTGGGCCCAAGCTTCTGGTGC AGGAGCCAGGAGGCCGCCAAGCTGTGCAACGCTGTGCAACACTGCCAGAAGCATGTATGGAAAGAGATGC ACCTCCACGCTGGGGAACACGCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC214503 representing NM_001085382
Red=Cloning site Green=Tags(s) MLCALLLLPSLLGATRASPTSGPQECAKGSTVWCQDLQTAARCGAVGYCQGAVWNKPTAKSLPCDVCQDI AAAAGNGLNPDATESDILALVMKTCEWLPSQESSAGCKWMVDAHSSAILSMLRGAPDSAPAQVCTALSLC EPLQRHLATLRPLSKEDTFEAVAPFMANGPLTFHPRQAPEGALCQDCVRQVSRLQEAVRSNLTLADLNIQ EQCESLGPGLAVLCKNYLFQFFVPADQALRLLPPQELCRKGGFCEELGAPARLTQVVAMDGVPSLELGLP RKQSEMQMKAGVTCEVCMNVVQKLDHWLMSNSSELMITHALERVCSVMPASITKECIILVDTYSPSLVQL VAKITPEKVCKFIRLCGNRRRARAVHDAYAIVPSPEWDAENQGSFCNGCKRLLTVSSHNLESKSTKRDIL VAFKGGCSILPLPYMIQCKHFVTQYEPVLIESLKDMMDPVAVCKKVGACHGPRTPLLGTDQCALGPSFWC RSQEAAKLCNAVQHCQKHVWKEMHLHAGEHA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001085382 |
| ORF Size | 1563 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001085382.2 |
| RefSeq Size | 4700 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 768239 |
| UniProt ID | Q6NUJ1 |
| Protein Pathways | Lysosome |
| MW | 56.4 kDa |
| Gene Summary | This gene encodes a protein that is related to the glycoprotein prosaposin. Based on sequence similarity between the encoded protein and prosaposin, it is predicted that the encoded protein is a preproprotein that is proteolytically processed to generate multiple protein products. These predicted products include saposins A-like, B-like, C-like, and D-like, which may play a role in the lysosomal degradation of sphingolipids. [provided by RefSeq, Jul 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214503L3 | Lenti-ORF clone of PSAPL1 (Myc-DDK-tagged)-Human prosaposin-like 1 (gene/pseudogene) (PSAPL1) |
CNY 6,460.00 |
|
| RC214503L4 | Lenti-ORF clone of PSAPL1 (mGFP-tagged)-Human prosaposin-like 1 (gene/pseudogene) (PSAPL1) |
CNY 6,460.00 |
|
| RG214503 | PSAPL1 (tGFP-tagged) - Human prosaposin-like 1 (gene/pseudogene) (PSAPL1) |
CNY 5,040.00 |
|
| SC316237 | PSAPL1 (untagged)-Human prosaposin-like 1 (gene/pseudogene) (PSAPL1) |
CNY 8,360.00 |
