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PHF21A (NM_016621) Human Tagged ORF Clone
CAT＃: RC214381
- TrueORF®
PHF21A (Myc-DDK-tagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2
ORF Plasmid: tGFP
"NM_016621" in other vectors (5)
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CNY 4,720.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BHC80; BM-006; IDDBCS; NEDMS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214381 representing NM_016621
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGCAGACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCTCAAA TGAAGCAGGATCCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTT GAGTGAGAAACAGAAAAGAGTAGTTGAACAGCTACGGAAGAACCTGATAGTAAAGCAAGAACAACCGGAC AAGTTCCAAATACAGCCATTGCCACAATCTGAAAACAAACTACAAACAGCACAGCAGCAACCACTACAGC AACTACAACAACAGCAGCAGTACCACCACCACCACGCCCAGCAGTCAGCTGCAGCCTCTCCCAACCTGAC TGCTTCACAGAAGACTGTAACTACAGCTTCTATGATTACCACAAAGACACTACCTCTCGTCTTGAAAGCA GCAACTGCGACCATGCCTGCCTCTGTGGTGGGCCAGAGACCTACCATTGCTATGGTGACCGCCATCAACA GTCAGAAGGCTGTGCTCAGCACTGATGTGCAGAACACACCAGTCAACCTCCAGACGTCTAGTAAGGTCAC TGGGCCTGGGGCAGAGGCTGTCCAAATTGTGGCAAAAAACACAGTCACTCTGCAGGTTCAGGCAACACCT CCTCAGCCCATCAAAGTACCACAGTTTATCCCCCCTCCTAGACTCACTCCACGTCCAAACTTTCTTCCAC AGGTTCGACCCAAGCCTGTGGCCCAGAATAACATTCCTATTGCCCCAGCACCACCTCCCATGCTCGCAGC TCCTCAGCTTATCCAGAGGCCCGTCATGCTGACCAAGTTCACCCCCACAACCCTTCCCACATCCCAGAAT TCCATCCACCCCGTCCGTGTCGTCAATGGGCAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGC TCACCAGCATTGTGATAGCTACTCCAGGGACCAGACTCGCTGGACCTCAAACTGTACAGCTTAGCAAGCC AAGTCTTGAAAAACAGACAGTTAAATCTCACACAGAAACAGATGAGAAACAAACAGAGAGCCGCACCATC ACCCCACCTGCTGCACCCAAACCAAAACGGGAGGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAG GGTTGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAAGAGCGAAAAAGAAGAACAACAGC AAATCCGGTCTACAGTGGAGCAGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTGACATACCTAAACAGC ACAATGCACCCTGGGACCCGGAAGAGAGCCAATGAGGAACACTGGCCAAAGGGTGATATTCATGAGGATT TTTGCAGCGTTTGCAGAAAAAGTGGCCAGTTACTGATGTGCGACACATGTTCCCGTGTATATCATTTGGA CTGCTTAGACCCCCCTCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATG CTGAAGAAGGAAGAAGCAATTCCATGGCCTGGAACTTTAGCAATTGTTCATTCCTATATTGCCTACAAAG CAGCAAAAGAAGAAGAGAAACAGAAGTTACTTAAATGGAGTTCAGATTTAAAACAAGAACGAGAACAACT AGAGCAAAAGGTGAAACAGCTCAGCAATTCCATAAGTAAATGCATGGAAATGAAGAACACCATCCTGGCC CGGCAGAAGGAGATGCACAGCTCCCTGGAGAAGGTAAAACAGCTGATTCGCCTCATCCACGGCATCGACC TCTCCAAACCTGTAGACTCTGAGGCCACTGTGGGGGCCATCTCCAATGGCCCGGACTGCACCCCCCCTGC CAATGCCGCCACCTCCACGCCGGCCCCTTCCCCCTCCTCCCAGAGCTGCACAGCGAACTGTAACCAGGGG GAAGAGACTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214381 representing NM_016621
Red=Cloning site Green=Tags(s) MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPD KFQIQPLPQSENKLQTAQQQPLQQLQQQQQYHHHHAQQSAAASPNLTASQKTVTTASMITTKTLPLVLKA ATATMPASVVGQRPTIAMVTAINSQKAVLSTDVQNTPVNLQTSSKVTGPGAEAVQIVAKNTVTLQVQATP PQPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQN SIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLAGPQTVQLSKPSLEKQTVKSHTETDEKQTESRTI TPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNS TMHPGTRKRANEEHWPKGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPRCQDQM LKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSISKCMEMKNTILA RQKEMHSSLEKVKQLIRLIHGIDLSKPVDSEATVGAISNGPDCTPPANAATSTPAPSPSSQSCTANCNQG EETK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016621 |
| ORF Size | 1902 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016621.5 |
| RefSeq Size | 7194 bp |
| RefSeq ORF | 1905 bp |
| Locus ID | 51317 |
| UniProt ID | Q96BD5 |
| Domains | PHD |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 70 kDa |
| Gene Summary | The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).[supplied by OMIM, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214381L3 | Lenti-ORF clone of PHF21A (Myc-DDK-tagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2 |
CNY 5,890.00 |
|
| RC214381L4 | Lenti-ORF clone of PHF21A (mGFP-tagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2 |
CNY 5,890.00 |
|
| RG214381 | PHF21A (tGFP-tagged) - Human PHD finger protein 21A (PHF21A), transcript variant 2 |
CNY 4,370.00 |
|
| SC114166 | PHF21A (untagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2 |
CNY 15,832.00 |
|
| SC318085 | PHF21A (untagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2 |
CNY 10,170.00 |
