TAOK2 (NM_004783) Human Tagged ORF Clone

CAT#: RC214344

TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_004783" in other vectors (7)

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CNY 7,304.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Anti-TAO2 (Ser181) Antibody (Phospho-Specific)
    • 100 ul

CNY 5,643.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214344 representing NM_004783
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACC
CAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGA
TGTCCGGAATAGTGAGGTGGTGGCCATCAAGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGG
CAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCT
GTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGA
AGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCA
TATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAGCCAGGGT
TAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGCACCCCATA
CTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGACGTCTGGTCC
TTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCT
TATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT
TGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTT
GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCCGTGCGGGAGC
TGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAACGGCCCTGGTGCCGA
GGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTGACCAGCCTCGAGAGT
AGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATG
CCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCG
GGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCG
GGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAGCCGCCTGCAG
CCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGCCGTAACCGAGACCACTTTGC
CACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCTGCGCTGCGGGAG
CAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGA
GGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGC
AGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAG
GAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTGGAGGCACAGA
AGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAGAACCCCAGCACTCCCAAGCG
GGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAGCAGTGCCAGGCGGAGGAGGAAGCAGGG
CTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTC
GGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTG
TGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGC
ACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTACAACAAGCGGC
GTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAATCTAAGGA
GCTGCAGATCAAGAAGCAGTTCCAGGAGACGTGTAAGATCCAGACTCGGCAGTACAAGGCTCTGCGAGCA
CACTTGCTGGAGACCACGCCCAAAGCTCAGCACAAGAGCCTCCTTAAGCGGCTCAAGGAAGAGCAGACCC
GCAAGCTGGCGATCTTGGCGGAGCAGTATGACCAGTCCATCTCAGAGATGCTCAGCTCACAGGCGCTGCG
GCTTGATGAGACCCAGGAGGCAGAGTTCCAGGCCCTTCGGCAGCAGCTTCAACAGGAGCTGGAGCTGCTC
AACGCTTACCAGAGCAAGATCAAGATCCGCACAGAGAGCCAGCACGAGAGGGAGCTGCGGGAGCTGGAGC
AGAGGGTCGCGCTGCGGCGGGCACTGCTGGAGCAGCGGGTGGAAGAGGAGCTGCTGGCCCTGCAGACAGG
ACGCTCCGAGCGAATCCGCAGTCTGCTTGAGCGGCAGGCCCGTGAGATCGAGGCCTTCGATGCGGAAAGC
ATGAGGCTGGGCTTCTCCAGCATGGCTCTGGGGGGCATCCCGGCTGAAGCTGCTGCCCAGGGCTATCCTG
CTCCACCCCCTGCCCCAGCCTGGCCCTCCCGTCCCGTTCCCCGTTCTGGGGCACACTGGAGCCATGGCCC
TCCTCCACCAGGCATGCCCCCTCCAGCCTGGCGTCAGCCGTCTCTGCTGGCTCCCCCAGGCCCCCCAAAC
TGGCTGGGGCCCCCCACACAAAGTGGGACACCCCGTGGCGGAGCCCTGCTGCTGCTAAGAAACAGCCCCC
AGCCCCTGCGGCGGGCAGCCTCGGGGGGCAGTGGCAGTGAGAATGTGGGCCCCCCTGCTGCCGCGGTGCC
CGGGCCCCTGAGCCGCAGCACCAGTGTCGCTTCCCACATCCTCAATGGTTCTTCCCACTTCTATTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214344 representing NM_004783
Red=Cloning site Green=Tags(s)

MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKW
QDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLA
YLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS
LGITCIELAERKPPLFNMNAMSALYHIAQNESPVLQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRF
VLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLES
SHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRLP
GSDNLYDDPYQPEITPSPLQPPAAPAPTSTTSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSALRE
QLSGYKRMRRQHQKQLLALESRLRGEREEHSARLQRELEAQRAGFGAEAEKLARRHQAIGEKEARAAQAE
ERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEEAG
LLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAVQR
TRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKSKELQIKKQFQETCKIQTRQYKALRA
HLLETTPKAQHKSLLKRLKEEQTRKLAILAEQYDQSISEMLSSQALRLDETQEAEFQALRQQLQQELELL
NAYQSKIKIRTESQHERELRELEQRVALRRALLEQRVEEELLALQTGRSERIRSLLERQAREIEAFDAES
MRLGFSSMALGGIPAEAAAQGYPAPPPAPAWPSRPVPRSGAHWSHGPPPPGMPPPAWRQPSLLAPPGPPN
WLGPPTQSGTPRGGALLLLRNSPQPLRRAASGGSGSENVGPPAAAVPGPLSRSTSVASHILNGSSHFYS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004783
ORF Size 3147 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004783.4
RefSeq Size 4625 bp
RefSeq ORF 3150 bp
Locus ID 9344
UniProt ID Q9UL54
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways MAPK signaling pathway
MW 119.1 kDa
Gene Summary This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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