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DAGLA (NM_006133) Human Tagged ORF Clone
CAT＃: RC214261
- TrueORF®
DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA)
ORF Plasmid: tGFP
"NM_006133" in other vectors (5)
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CNY 7,256.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C11orf11; DAGL(ALPHA); DAGLALPHA; NSDDR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214261 representing NM_006133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGTGATGACCTCGTCCTACCGGCCATCT TCCTCTTTCTCCTGCATACCACCTGGTTTGTGATCCTGTCCGTGGTGCTCTTCGGCCTGGTCTATAACCC GCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGCGGCTACCTGGGCATCCTGCTGAGCTGCATG ATCGCTGAGATGGCCATCATCTGGCTGAGCATGCGCGGGGGCATCCTCTACACGGAGCCCCGTGACTCCA TGCAGTACGTGCTCTACGTGCGCCTGGCCATCCTGGTGATCGAGTTCATCTACGCCATCGTGGGCATCGT CTGGCTCACTCAGTACTACACCTCCTGCAACGACCTCACTGCCAAGAATGTCACCCTCGGAATGGTTGTC TGCAACTGGGTAGTCATCCTCAGTGTGTGCATCACTGTCCTCTGCGTCTTCGACCCCACGGGCCGCACCT TTGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGTAACCTGCGGACCTACAACCTGCGGCACCGCTTAGA GGAGGGTCAAGCCACCAGCTGGTCGCGCCGGCTCAAAGTGTTCCTCTGCTGCACGCGGACGAAGGACTCC CAGTCAGATGCCTACTCAGAAATCGCCTACCTCTTTGCGGAGTTCTTCCGGGACCTTGACATTGTGCCAT CCGACATCATTGCTGGCCTGGTGCTGCTCCGGCAGCGGCAGCGGGCCAAGCGCAACGCCGTGCTGGACGA GGCAAACAATGACATCTTGGCCTTCCTGTCTGGGATGCCGGTGACCAGAAACACCAAGTACCTCGACCTC AAGAATTCACAAGAGATGCTCCGCTACAAAGAGGTCTGCTACTACATGCTCTTTGCCCTGGCTGCCTACG GGTGGCCCATGTACCTGATGCGGAAGCCCGCCTGCGGCCTCTGCCAACTGGCTCGGTCCTGCTCGTGTTG CCTGTGTCCTGCGAGGCCGCGGTTCGCCCCTGGAGTCACCATCGAGGAAGACAACTGCTGTGGCTGTAAT GCCATTGCCATCCGGCGCCACTTCCTGGACGAGAACATGACTGCGGTGGACATCGTCTATACCTCCTGCC ATGATGCGGTCTATGAAACGCCCTTCTACGTGGCGGTGGACCATGACAAGAAGAAAGTGGTGATCAGTAT CCGGGGGACCCTGTCCCCCAAGGATGCCCTGACTGACCTGACGGGTGATGCTGAGCGCCTCCCCGTGGAG GGGCACCACGGCACCTGGCTGGGCCACAAGGGTATGGTCCTCTCAGCTGAGTACATCAAGAAGAAACTGG AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGCGGAACCAAACACTACGGCCTGAT TGTGGTGGGCCACTCCCTGGGCGCGGGCACTGCTGCCATCCTCTCCTTCCTTCTGCGCCCACAGTATCCG ACCCTCAAGTGCTTTGCCTACTCCCCGCCAGGGGGCCTGCTGAGTGAGGATGCGATGGAGTATTCCAAGG AGTTCGTGACTGCTGTGGTTCTGGGCAAAGACCTCGTCCCCAGGATTGGCCTCTCTCAGCTGGAAGGCTT CCGCAGACAGCTCCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC AAATGCATCCCCAAGTCGGAGCTGCCTGAGGAGGTAGAGGTGACCACCCTGGCCAGCACGCGGCTCTGGA CCCACCCCAGCGACCTAACTATAGCCCTCTCAGCCAGCACTCCACTCTACCCGCCCGGCCGCATCATCCA CGTGGTCCACAACCACCCTGCAGAGCAGTGCTGCTGCTGTGAGCAGGAGGAGCCCACATACTTTGCCATC TGGGGCGACAACAAGGCCTTCAATGAGGTGATCATCTCGCCAGCCATGCTGCATGAGCACCTGCCCTATG TGGTCATGGAGGGGCTCAACAAGGTGCTGGAGAACTACAACAAGGGGAAGACCGCTCTGCTCTCTGCAGC CAAGGTCATGGTGAGCCCTACCGAGGTGGACCTGACTCCTGAGCTCATCTTCCAGCAGCAGCCACTCCCC ACGGGGCCGCCCATGCCCACTGGCCTTGCCCTGGAGCTGCCGACTGCAGACCACCGCAACAGCAGCGTCA GGAGCAAGTCCCAGTCTGAGATGAGCCTGGAGGGCTTCTCGGAGGGGCGGCTGCTGTCGCCAGTGGTTGC GGCGGCGGCCCGCCAGGACCCGGTGGAGCTGCTGCTGCTGTCTACCCAGGAGCGGCTGGCGGCGGAGCTG CAGGCCCGGCGGGCACCACTGGCCACCATGGAGAGCCTCTCGGACACTGAGTCCCTGTACAGCTTCGACT CGCGCCGCTCCTCAGGCTTCCGCAGCATCCGGGGCTCCCCCAGCCTCCACGCTGTGCTGGAGCGTGATGA AGGCCACCTCTTCTACATTGACCCTGCCATCCCCGAGGAAAACCCATCCCTGAGCTCGCGCACTGAGCTG CTGGCGGCCGACAGCCTGTCCAAGCACTCACAGGACACGCAGCCCCTGGAGGCGGCCCTGGGCAGTGGCG GCGTCACTCCTGAGCGGCCCCCCAGTGCTGCGGCCAATGACGAGGAGGAAGAGGTTGGCGGTGGGGGTGG CGGGCCGGCCTCCCGCGGGGAGCTGGCGCTGCACAATGGGCGCCTGGGGGACTCGCCCAGTCCTCAGGTG CTGGAATTCGCCGAGTTCATCGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAAGACCTCT ACTGCATGGTGGTGCCCGAGAGCCCCACCAGTGACTACGCTGAGGGCCCCAAGTCCCCCAGCCAGCAAGA GATCCTGCTCCGTGCCCAGTTCGAGCCCAACCTGGTGCCCAAGCCCCCACGGCTCTTTGCCGGCTCAGCC GACCCCTCCTCGGGCATCTCACTCTCGCCCTCCTTCCCGCTCAGCTCCTCGGGTGAGCTCATGGACCTGA CGCCCACGGGCCTCAGTAGCCAGGAATGCCTGGCGGCTGACAAGATCCGGACTTCTACCCCCACTGGCCA CGGAGCCAGCCCCGCCAAGCAAGATGAGCTGGTCATCTCAGCACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214261 representing NM_006133
Red=Cloning site Green=Tags(s) MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCM IAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVV CNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDS QSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDL KNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCN AIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVE GHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYP TLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGAT KCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAI WGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLP TGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAEL QARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTEL LAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQV LEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSA DPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006133 |
| ORF Size | 3126 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006133.3 |
| RefSeq Size | 5761 bp |
| RefSeq ORF | 3129 bp |
| Locus ID | 747 |
| UniProt ID | Q9Y4D2 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 115 kDa |
| Gene Summary | This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214261L3 | Lenti-ORF clone of DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA) |
CNY 10,260.00 |
|
| RC214261L4 | Lenti-ORF clone of DAGLA (mGFP-tagged)-Human diacylglycerol lipase, alpha (DAGLA) |
CNY 10,260.00 |
|
| RG214261 | DAGLA (tGFP-tagged) - Human diacylglycerol lipase, alpha (DAGLA) |
CNY 9,220.00 |
|
| SC101029 | DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA) |
CNY 7,264.00 |
|
| SC309995 | DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA) |
CNY 10,450.00 |
