Periplakin (PPL) (NM_002705) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213952 representing NM_002705 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCCACTGTGCAGACCCGGAGCATCTCTA ACAAGGAGCTCTCGGAGCTGATCGAGCAGCTGCAGAAGAATGCCGACCAGGTGGAGAAGAACATCGTGGA CACAGAGGCCAAGATGCAGAGTGACCTGGCTCGGCTGCAGGAGGGTCGGCAGCCTGAGCACCGGGACGTG ACCCTGCAGAAGGTGTTGGACTCTGAGAAGCTGCTCTATGTGCTAGAGGCGGATGCGGCCATTGCCAAGC ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGATATCCGCCAGCTGAAGGAGCGTGTGACCAACCT GCGCGGGAAACACAAGCAGATCTACAGGCTGGCGGTGAAGGAAGTGGATCCACAGGTCAACTGGGCGGCA CTGGTGGAGGAGAAGCTGGACAAGCTGAACAACCAGAGCTTTGGGACTGACCTGCCGCTGGTGGACCACC AAGTGGAGGAGCATAACATCTTCCACAATGAGGTCAAGGCCATCGGGCCCCACCTGGCCAAGGACGGGGA CAAGGAGCAGAACAGCGAACTCCGGGCCAAGTACCAGAAACTGCTGGCAGCATCACAGGCCCGGCAGCAG CACCTGAGTTCGCTGCAGGACTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAGGCCA AGGGCCGCATGCAGTACGACTGGAGTGACCGCAACCTCGACTACCCCAGCCGCCGGCGCCAGTATGAGAA TTTCATCAACCGGAACCTGGAGGCCAAAGAGGAGAGAATCAACAAACTGCACAGCGAGGGCGACCAGCTG CTGGCGGCCGAGCACCCCGGGAGGAACTCCATTGAGGCGCACATGGAGGCTGTGCACGCAGACTGGAAGG AGTACCTGAACCTGCTCATCTGCGAGGAGAGCCACCTCAAGTACATGGAGGACTACCACCAGTTTCACGA AGACGTGAAGGACGCTCAGGAGCTGCTGCGCAAGGTGGACTCGGACCTGAACCAGAAGTATGGCCCTGAC TTCAAGGACCGGTACCAGATTGAGCTGCTGCTGCGGGAGCTGGATGACCAGGAGAAGGTGCTGGACAAGT ATGAGGACGTGGTGCAGGGGCTGCAGAAGCGAGGCCAGCAGGTGGTGCCCCTCAAGTACCGCCGGGAGAC TCCGCTCAAGCCCATCCCCGTGGAGGCACTCTGTGACTTTGAGGGGGAGCAGGGCCTGATCTCGCGGGGC TACAGCTACACCCTGCAGAAGAACAACGGGGAGAGCTGGGAGCTCATGGACAGCGCTGGGAACAAGCTGA TTGCTCCGGCCGTCTGTTTTGTGATCCCCCCCACAGACCCTGAGGCCCTGGCTCTGGCTGACAGCCTGGG CAGCCAGTACCGGAGCGTGCGGCAGAAGGCAGCTGGGAGCAAACGCACGCTGCAGCAGCGGTATGAGGTG CTGAAGACCGAGAATCCCGGAGATGCCTCTGACCTACAGGGGCGGCAGCTGCTGGCTGGCTTGGACAAGG TGGCCAGCGACCTGGACCGGCAGGAGAAGGCCATCACAGGGATCCTGCGGCCACCACTGGAGCAAGGCCG GGCTGTGCAGGACAGTGCCGAGCGGGCCAAGGACCTCAAGAACATCACCAACGAGCTACTGCGGATTGAA CCTGAGAAGACGCGGAGCACGGCTGAGGGCGAAGCCTTCATCCAGGCCCTCCCAGGCAGTGGCACCACAC CCCTGCTGAGGACCCGGGTGGAGGACACCAACCGGAAATACGAGCACCTCCTGCAGCTGCTGGACTTGGC CCAGGAGAAGGTTGATGTGGCCAACCGCCTGGAGAAGAGCCTGCAGCAGAGCTGGGAGTTGCTGGCCACA CACGAGAACCATCTGAATCAGGATGACACAGTGCCTGAGAGCAGCCGTGTCCTGGACAGCAAGGGGCAGG AGCTGGCGGCCATGGCCTGTGAGTTACAGGCCCAGAAGTCCCTCCTGGGTGAGGTGGAGCAGAACTTGCA GGCGGCCAAGCAGTGCTCGAGCACACTGGCCAGCCGCTTCCAGGAGCACTGCCCGGACCTGGAGCGCCAG GAGGCCGAGGTGCACAAGCTGGGCCAGCGTTTCAACAACCTGCGCCAGCAGGTGGAACGCAGGGCGCAGA GCCTACAGAGCGCCAAGGCAGCCTACGAGCACTTCCACCGCGGCCATGACCACGTGCTGCAGTTCCTAGT CAGCATCCCCAGTTACGAGCCCCAGGAGACAGACAGCCTCAGCCAGATGGAGACCAAGCTGAAGAACCAG AAGAACCTGCTAGATGAGATAGCAAGTAGGGAGCAGGAAGTACAGAAGATCTGTGCCAATTCCCAGCAGT ACCAGCAAGCTGTAAAGGACTATGAGTTAGAAGCAGAAAAACTAAGGTCTCTTCTCGACTTGGAGAATGG AAGGAGCAGCCACGTGAGCAAGAGAGCCAGGCTCCAATCTCCTGCCACCAAAGTGAAGGAAGAGGAAGCA GCACTTGCCGCCAAGTTCACTGAAGTTTATGCCATCAACAGACAGAGGCTGCAGAATCTGGAGTTTGCTC TGAATCTCCTCAGACAGCAGCCGGAAGTAGAAGTGACCCATGAGACCCTGCAAAGGAATAGGCCGGACTC TGGAGTGGAGGAGGCGTGGAAGATCAGGAAGGAACTGGATGAGGAGACTGAGCGGAGGCGGCAGCTGGAG AACGAGGTCAAGAGCACCCAGGAAGAAATCTGGACCTTGAGGAATCAGGGGCCTCAGGAATCGGTGGTGA GGAAGGAGGTGCTCAAGAAGGTGCCGGATCCCGTGCTGGAGGAGAGCTTCCAGCAGCTGCAGCGGACGCT GGCAGAGGAGCAGCACAAGAACCAGCTGCTGCAGGAGGAGCTGGAGGCACTGCAGCTGCAGCTGCGTGCC CTGGAGCAGGAGACCAGAGACGGGGGGCAGGAGTACGTGGTCAAGGAGGTCCTGCGCATCGAGCCTGACA GGGCCCAGGCGGATGAGGTCTTGCAGCTGCGGGAGGAGCTGGAGGCACTGAGGCGGCAGAAGGGCGCCCG GGAGGCAGAGGTGCTCCTCCTGCAGCAGCGTGTGGCCGCCCTGGCTGAAGAGAAGAGCCGGGCGCAGGAG AAGGTCACAGAGAAAGAGGTGGTGAAACTGCAGAATGACCCCCAGCTGGAGGCAGAGTACCAGCAGCTGC AGGAGGACCACCAGCGCCAGGACCAGCTCAGGGAGAAGCAGGAGGAGGAGCTGAGCTTCCTCCAGGACAA GCTCAAGAGGCTAGAGAAGGAGCGGGCCATGGCCGAGGGCAAGATCACCGTCAAGGAGGTGCTCAAGGTG GAGAAGGACGCGGCCACCGAGAGGGAGGTCAGCGATCTCACCCGCCAATATGAGGACGAGGCTGCCAAGG CTCGCGCTAGCCAGAGGGAGAAGACGGAGCTGCTCCGAAAGATATGGGCCTTGGAGGAGGAGAACGCCAA AGTGGTGGTGCAGGAGAAGGTGCGGGAGATCGTGCGGCCAGACCCCAAGGCGGAAAGTGAAGTGGCGAAC CTCCGCCTGGAGCTTGTGGAGCAGGAGCGAAAGTACCGGGGTGCCGAGGAGCAGCTCCGGAGCTACCAGA GTGAGCTGGAGGCCCTCAGGAGGCGAGGCCCCCAGGTGGAAGTCAAAGAGGTGACTAAGGAAGTCATTAA GTACAAGACTGACCCTGAGATGGAGAAGGAGCTTCAGCGGCTCAGGGAGGAGATCGTGGACAAGACCAGA CTGATCGAAAGGTGTGATTTAGAGATCTACCAGCTGAAAAAGGAAATCCAGGCCCTGAAAGACACCAAAC CCCAGGTCCAGACCAAAGAGGTGGTCCAGGAGATCCTCCAATTCCAAGAAGACCCTCAAACCAAGGAGGA GGTGGCGTCTCTGAGGGCAAAGCTCTCAGAGGAGCAGAAGAAACAAGTGGATCTGGAGAGGGAAAGAGCT TCCCAGGAAGAGCAGATCGCCCGGAAAGAGGAGGAGCTCTCGCGGGTGAAGGAAAGGGTGGTGCAGCAGG AGGTGGTCAGGTATGAGGAGGAGCCAGGCCTGCGGGCCGAGGCGAGCGCCTTTGCCGAGAGCATCGATGT GGAGCTGCGGCAGATTGACAAGCTGCGGGCAGAGCTGCGGCGGCTGCAGCGCCGGCGCACCGAGCTTGAG CGGCAGCTGGAGGAGCTAGAGCGCGAGCGGCAGGCCCGCAGGGAGGCCGAGCGCGAGGTACAGCGGTTGC AGCAGCGGCTGGCAGCGCTGGAGCAGGAAGAAGCTGAGGCCCGTGAGAAGGTAACCCATACGCAGAAGGT GGTGCTGCAGCAGGACCCGCAGCAGGCGCGAGAGCATGCCCTGCTCCGACTCCAGCTGGAAGAAGAGCAG CACCGGCGGCAGCTCCTGGAGGGGGAGCTCGAGACCCTCCGGAGGAAACTGGCTGCACTGGAGAAGGCGG AGGTCAAGGAGAAGGTGGTGCTCTCCGAGAGTGTCCAGGTGGAGAAGGGCGACACCGAGCAAGAGATCCA GAGGCTCAAGAGCAGCCTGGAGGAGGAGAGCCGCAGCAAGCGCGAGCTGGACGTCGAGGTGAGCCGGCTG GAAGCCAGGCTTTCGGAGCTGGAATTCCATAACTCCAAGTCATCCAAGGAACTAGACTTTCTGAGGGAAG AGAACCACAAATTACAGCTGGAGAGGCAAAACCTGCAGCTGGAGACCCGAAGGCTCCAATCGGAAATCAA CATGGCAGCGACGGAAACACGAGACCTGCGGAACATGACCGTGGCGGACTCTGGGACCAACCATGACTCC AGACTGTGGTCCCTGGAGAGGGAACTGGATGACCTCAAGAGGCTCTCCAAGGACAAAGACCTCGAGATCG ACGAGCTGCAGAAGCGCCTGGGCTCCGTGGCCGTCAAGCGGGAGCAGCGGGAGAACCACCTGCGGCGCTC CATCGTAGTCATCCACCCTGACACAGGCCGCGAGCTGTCCCCGGAGGAAGCCCACCGTGCCGGGCTCATT GACTGGAACATGTTCGTGAAACTCAGAAGCCAGGAGTGCGACTGGGAGGAGATCTCAGTGAAGGGTCCCA ATGGGGAGTCCTCAGTGATACACGACAGGAAGTCTGGCAAGAAGTTCTCCATCGAAGAGGCCCTGCAGAG TGGCAGGCTGACCCCTGCTCAGTATGACCGCTATGTCAACAAGGATATGTCCATCCAGGAGCTGGCGGTC TTGGTATCTGGGCAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC213952 representing NM_002705 Red=Cloning site Green=Tags(s) MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDV TLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAA LVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQ HLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQL LAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPD FKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRG YSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEV LKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIE PEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLAT HENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQ EAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQ KNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVKEEEA ALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLE NEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRA LEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQE KVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKV EKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVAN LRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTR LIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERA SQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELE RQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQ HRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRL EARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDS RLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLI DWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAV LVSGQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002705
ORF Size	5268 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002705.3, NP_002696.3
RefSeq Size	6256 bp
RefSeq ORF	5271 bp
Locus ID	5493
UniProt ID	O60437
MW	204.5 kDa
Gene Summary	The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008]

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