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MRE11 (NM_005590) Human Tagged ORF Clone
CAT＃: RC213729
- TrueORF®
MRE11A (Myc-DDK-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2
ORF Plasmid: tGFP
"NM_005590" in other vectors (6)
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CNY 7,968.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATLD; HNGS1; MRE11A; MRE11B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213729 representing NM_005590
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGATATTCATCTTG GATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAAATTTTAAGACTTGC CCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGGAAA ACATTACATACCTGCCTCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTC TCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAA CATTTCAATTCCAGTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCC TTGGACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAGATAGACA TTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTAGGATCCATTCCAGATGA AAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCTTGG TTTAACTTATTTGTGATTCATCAGAACAGGAGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTT TGGATGACTTCATTGATCTTGTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGA ACAACAGCTGTTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCTCTTCACACAG TGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATTTTTAACCCAGATAATCCTAA AGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATTGAAGAAATGCTTGAAAATGCTGAACGGGAA CGTCTGGGTAATTCTCACCAGCCAGAGAAGCCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTG AACCTTTCAGTGTTCTTCGCTTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCA TTTTTTCAGGCATAGAGAACAAAAGGAAAAAACAGGAGAAGAGATCAACTTTGGGAAACTTATCACAAAG CCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGCAGAGAAGAATG TGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAATTTGTGGACAAGGAGGAGAA AGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAAAACACAGCGATTTCTTAAAGAACGTCATATT GATGCCCTCGAAGACAAAATCGATGAGGAGGTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATG AAGAAGATGATGAAGTCCGTGAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGC TTCTGCCTTTAGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGAT AGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGAGGGCAGAATT CAGCATCGAGAGGAGGGTCTCAAAGAGGAAGAGCCTTTAAATCTACAAGACAGCAGCCTTCCCGAAATGT CACTACTAAGAATTATTCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAGACATTTTTCCTACC ACTTCAAAGACAGATCAAAGGTGGTCCAGCACATCATCCAGCAAAATCATGTCCCAGAGTCAAGTATCGA AAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTTTTATGAACACTAGTTCTTTAAGAAG AAATAGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213729 representing NM_005590
Red=Cloning site Green=Tags(s) MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRK TLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCA LDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSW FNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAV KKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERE RLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITK PSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHI DALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDD SISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPT TSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005590 |
| ORF Size | 2040 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005590.4 |
| RefSeq Size | 5164 bp |
| RefSeq ORF | 2043 bp |
| Locus ID | 4361 |
| UniProt ID | P49959 |
| Domains | Metallophos, Mre11_DNA_bind |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination, Non-homologous end-joining |
| MW | 77.5 kDa |
| Gene Summary | This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213729L1 | Lenti-ORF clone of MRE11A (Myc-DDK-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 10,368.00 |
|
| RC213729L2 | Lenti-ORF clone of MRE11A (mGFP-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 9,120.00 |
|
| RC213729L3 | Lenti-ORF clone of MRE11A (Myc-DDK-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 9,120.00 |
|
| RC213729L4 | Lenti-ORF clone of MRE11A (mGFP-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 9,120.00 |
|
| RG213729 | MRE11A (tGFP-tagged) - Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 7,980.00 |
|
| SC126932 | MRE11A (untagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2 |
CNY 7,480.00 |
