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PHC2 (NM_004427) Human Tagged ORF Clone
CAT＃: RC212697
PHC2 (Myc-DDK-tagged)-Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2
ORF Plasmid: tGFP
"NM_004427" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EDR2; HPH2; PH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212697 representing NM_004427
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCAGGGAACGGAAACTCTGCCTCCAGCATCGCCGGCACTGCCCCCCAGAATGGTGAGAATAAAC CACCACAGGCCATTGTGAAACCCCAAATCCTGACGCATGTTATCGAAGGGTTTGTGATCCAGGAGGGGGC GGAGCCTTTCCCGGTGGGACGCTCGTCCCTGCTGGTGGGGAATCTCAAGAAGAAGTATGCACAGGGGTTC CTGCCTGAGAAACTTCCACAGCAGGATCACACCACCACCACTGACTCGGAGATGGAGGAGCCCTATCTGC AAGAATCCAAAGAGGAGGGTGCTCCCCTCAAACTCAAGTGTGAGCTCTGTGGCCGGGTGGACTTTGCCTA TAAGTTCAAGCGTTCCAAGCGCTTCTGTTCCATGGCTTGTGCAAAGAGGTACAACGTGGGATGCACCAAA CGGGTGGGACTTTTCCACTCAGACCGGAGCAAGCTGCAGAAGGCAGGAGCTGCGACCCACAACCGCCGTC GGGCCAGCAAAGCCAGTCTGCCACCACTTACCAAGGATACCAAGAAGCAGCCAACAGGCACTGTGCCCCT TTCGGTTACTGCTGCTTTGCAGCTAACACACAGCCAGGAAGACTCCAGCCGTTGCTCAGATAACTCAAGC TATGAGGAACCCTTGTCACCCATCTCAGCCAGCTCATCTACTTCCCGCCGGCGACAAGGCCAGCGGGACC TGGAGCTCCCCGACATGCATATGCGGGACCTGGTGGGCATGGGACACCACTTCCTGCCAAGTGAGCCCAC CAAGTGGAATGTAGAAGACGTCTACGAATTCATCCGCTCTCTGCCAGGCTGCCAGGAGATAGCAGAGGAA TTCCGTGCCCAGGAAATCGACGGGCAAGCCCTGCTGCTGCTCAAGGAGGACCACCTGATGAGCGCCATGA ACATCAAGCTGGGGCCCGCCCTGAAGATCTATGCCCGCATCAGCATGCTCAAGGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212697 representing NM_004427
Red=Cloning site Green=Tags(s) MTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGF LPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTK RVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSS YEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEE FRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004427 |
| ORF Size | 969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004427.4 |
| RefSeq Size | 2597 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 1912 |
| UniProt ID | Q8IXK0 |
| MW | 35.6 kDa |
| Gene Summary | In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212697L3 | Lenti ORF clone of Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212697L4 | Lenti ORF clone of Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212697 | PHC2 (tGFP-tagged) - Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2 |
CNY 4,370.00 |
|
| SC312947 | PHC2 (untagged)-Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2 |
CNY 6,270.00 |
