PLAAT2 (NM_017878) Human Tagged ORF Clone

CAT#: RC212578

HRASLS2 (Myc-DDK-tagged)-Human HRAS-like suppressor 2 (HRASLS2)

ORF Plasmid: DDK tGFP



  "NM_017878" in other vectors (6)

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CNY 3,705.00


货期*
3周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-HRASLS2 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HRASLS2; PLA1/2-2; PLAAT-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212578 representing NM_017878
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTGGCCAGACCAAGACCGAGACTTGGAGACCTGATTGAGATTTCTCGCTTTGGCTATGCACACT
GGGCCATCTACGTGGGAGATGGCTATGTGGTCCATCTGGCTCCGGCAAGTGAAATTGCTGGAGCTGGTGC
GGCCAGTGTCCTGTCTGCCCTGACCAACAAAGCCATAGTGAAGAAGGAACTGCTGTCTGTGGTGGCTGGG
GGAGACAACTACAGGGTCAATAACAAGCACGATGACAGATACACACCACTGCCTTCCAACAAAATCGTCA
AGCGGGCAGAGGAGTTGGTGGGGCAGGAGTTGCCTTATTCGCTGACCAGTGACAACTGCGAGCACTTCGT
GAACCATCTGCGCTATGGCGTCTCCCGCAGTGACCAGGTCACTGGTGCAGTCACGACAGTAGGTGTGGCA
GCAGGCCTGCTGGCTGCCGCAAGCCTTGTGGGGATCCTGCTGGCCAGAAGCAAGCGGGAAAGGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212578 representing NM_017878
Red=Cloning site Green=Tags(s)

MALARPRPRLGDLIEISRFGYAHWAIYVGDGYVVHLAPASEIAGAGAASVLSALTNKAIVKKELLSVVAG
GDNYRVNNKHDDRYTPLPSNKIVKRAEELVGQELPYSLTSDNCEHFVNHLRYGVSRSDQVTGAVTTVGVA
AGLLAAASLVGILLARSKRERQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017878
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017878.2
RefSeq Size 785 bp
RefSeq ORF 489 bp
Locus ID 54979
UniProt ID Q9NWW9
Protein Families Druggable Genome, Transmembrane
MW 17.2 kDa
Gene Summary The protein encoded by this gene has both phospholipase and acyltransferase activities and acts as a tumor suppressor. The encoded protein can hydrolyze dipalmitoylated phosphatidylcholine (PC) to palmitic acid and lyso-PC. In addition, this protein can catalyze the N-acylation of phosphatidylethanolamine and can catalyze the O-acylation of lyso-PC to form PC. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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