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BMP1 (NM_001199) Human Tagged ORF Clone
CAT＃: RC212538
BMP1 (Myc-DDK-tagged)-Human bone morphogenetic protein 1 (BMP1), transcript variant 1
ORF Plasmid: tGFP
"NM_001199" in other vectors (6)
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CNY 8,016.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OI13; PCOLC; PCP; PCP2; TLD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212538 representing NM_001199
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGCGTGGCCCGCCTGCCGCTGCTGCTCGGGCTGCTGCTGCTCCCGCGTCCCGGCCGGCCGCTGG ACTTGGCCGACTACACCTATGACCTGGCGGAGGAGGACGACTCGGAGCCCCTCAACTACAAAGACCCCTG CAAGGCGGCTGCCTTTCTTGGGGACATTGCCCTGGACGAAGAGGACCTGAGGGCCTTCCAGGTACAGCAG GCTGTGGATCTCAGACGGCACACAGCTCGTAAGTCCTCCATCAAAGCTGCAGTTCCAGGAAACACTTCTA CCCCCAGCTGCCAGAGCACCAACGGGCAGCCTCAGAGGGGAGCCTGTGGGAGATGGAGAGGTAGATCCCG TAGCCGGCGGGCGGCGACGTCCCGACCAGAGCGTGTGTGGCCCGATGGGGTCATCCCCTTTGTCATTGGG GGAAACTTCACTGGTAGCCAGAGGGCAGTCTTCCGGCAGGCCATGAGGCACTGGGAGAAGCACACCTGTG TCACCTTCCTGGAGCGCACTGACGAGGACAGCTATATTGTGTTCACCTATCGACCTTGCGGGTGCTGCTC CTACGTGGGTCGCCGCGGCGGGGGCCCCCAGGCCATCTCCATCGGCAAGAACTGTGACAAGTTCGGCATT GTGGTCCACGAGCTGGGCCACGTCGTCGGCTTCTGGCACGAACACACTCGGCCAGACCGGGACCGCCACG TTTCCATCGTTCGTGAGAACATCCAGCCAGGGCAGGAGTATAACTTCCTGAAGATGGAGCCTCAGGAGGT GGAGTCCCTGGGGGAGACCTATGACTTCGACAGCATCATGCATTACGCTCGGAACACATTCTCCAGGGGC ATCTTCCTGGATACCATTGTCCCCAAGTATGAGGTGAACGGGGTGAAACCTCCCATTGGCCAAAGGACAC GGCTCAGCAAGGGGGACATTGCCCAAGCCCGCAAGCTTTACAAGTGCCCAGCCTGTGGAGAGACCCTGCA AGACAGCACAGGCAACTTCTCCTCCCCTGAATACCCCAATGGCTACTCTGCTCACATGCACTGCGTGTGG CGCATCTCTGTCACACCCGGGGAGAAGATCATCCTGAACTTCACGTCCCTGGACCTGTACCGCAGCCGCC TGTGCTGGTACGACTATGTGGAGGTCCGAGATGGCTTCTGGAGGAAGGCGCCCCTCCGAGGCCGCTTCTG CGGGTCCAAACTCCCTGAGCCTATCGTCTCCACTGACAGCCGCCTCTGGGTTGAATTCCGCAGCAGCAGC AATTGGGTTGGAAAGGGCTTCTTTGCAGTCTACGAAGCCATCTGCGGGGGTGATGTGAAAAAGGACTATG GCCACATTCAATCGCCCAACTACCCAGACGATTACCGGCCCAGCAAAGTCTGCATCTGGCGGATCCAGGT GTCTGAGGGCTTCCACGTGGGCCTCACATTCCAGTCCTTTGAGATTGAGCGCCACGACAGCTGTGCCTAC GACTATCTGGAGGTGCGCGACGGGCACAGTGAGAGCAGCACCCTCATCGGGCGCTACTGTGGCTATGAGA AGCCTGATGACATCAAGAGCACGTCCAGCCGCCTCTGGCTCAAGTTCGTCTCTGACGGGTCCATTAACAA AGCGGGCTTTGCCGTCAACTTTTTCAAAGAGGTGGACGAGTGCTCTCGGCCCAACCGCGGGGGCTGTGAG CAGCGGTGCCTCAACACCCTGGGCAGCTACAAGTGCAGCTGTGACCCCGGGTACGAGCTGGCCCCAGACA AGCGCCGCTGTGAGGCTGCCTGTGGCGGATTCCTCACCAAGCTCAACGGCTCCATCACCAGCCCGGGCTG GCCCAAGGAGTACCCCCCCAACAAGAACTGCATCTGGCAGCTGGTGGCCCCCACCCAGTACCGCATCTCC CTGCAGTTTGACTTCTTTGAGACAGAGGGCAATGATGTGTGCAAGTACGACTTCGTGGAGGTGCGCAGTG GACTCACAGCTGACTCCAAGCTGCATGGCAAGTTCTGTGGTTCTGAGAAGCCCGAGGTCATCACCTCCCA GTACAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTGTCCAAAAAGGGCTTCAAGGCCCACTTC TTCTCAGAAAAGAGGCCAGCTCTGCAGCCCCCTCGGGGACGCCCCCACCAGCTCAAATTCCGAGTGCAGA AAAGAAACCGGACCCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212538 representing NM_001199
Red=Cloning site Green=Tags(s) MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQ AVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIG GNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGI VVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRG IFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVW RISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSS NWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAY DYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCE QRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRIS LQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHF FSEKRPALQPPRGRPHQLKFRVQKRNRTPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199 |
| ORF Size | 2190 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001199.4 |
| RefSeq Size | 2487 bp |
| RefSeq ORF | 2193 bp |
| Locus ID | 649 |
| UniProt ID | P13497 |
| Domains | CUB, Astacin, EGF_CA, ZnMc, EGF, EGF |
| Protein Families | Druggable Genome, Protease |
| MW | 82.9 kDa |
| Gene Summary | This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail
,Rosell-García, T;Rodríguez-Pascual, F;,
Methods in Cell Biology
[BMP1]
|
|
Cross-talk between miR-29 and Transforming Growth Factor-Betas in Trabecular Meshwork Cells
,Coralia Luna, Guorong Li, Jianming Qiu, David L. Epstein, and Pedro Gonzalez,
Invest. Ophthalmol. Vis. Sci., Jun 2011; 52: 3567 - 3572
[BMP1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212538L1 | Lenti ORF clone of Human bone morphogenetic protein 1 (BMP1), transcript variant 1, Myc-DDK-tagged |
CNY 10,416.00 |
|
| RC212538L2 | Lenti ORF clone of Human bone morphogenetic protein 1 (BMP1), transcript variant 1, mGFP tagged |
CNY 9,600.00 |
|
| RC212538L3 | Lenti ORF clone of Human bone morphogenetic protein 1 (BMP1), transcript variant 1, Myc-DDK-tagged |
CNY 10,416.00 |
|
| RC212538L4 | Lenti ORF clone of Human bone morphogenetic protein 1 (BMP1), transcript variant 1, mGFP tagged |
CNY 10,416.00 |
|
| RG212538 | BMP1 (tGFP-tagged) - Human bone morphogenetic protein 1 (BMP1), transcript variant 1 |
CNY 9,616.00 |
|
| SC302989 | BMP1 (untagged)-Human bone morphogenetic protein 1 (BMP1), transcript variant 1 |
CNY 8,032.00 |
