Myoferlin (MYOF) (NM_133337) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FER1L3; HAE7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212351 representing NM_133337 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGAGTGATTGTGGAATCTGCCAGCAATATCCCTAAAACGAAATTTGGCAAGCCGGATCCTATTG TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAATTGAACCCTGTCTGGAA TGAGATTTTGGAGTTTGACTTGAGGGGTATACCACTGGACTTTTCATCTTCCCTTGGGATTATTGTGAAA GATTTTGAGACAATTGGACAAAATAAATTAATTGGCACGGCGACTGTAGCCCTGAAGGACCTGACTGGTG ACCAGAGCAGATCCCTGCCGTACAAGCTGATCTCCCTGCTAAATGAAAAAGGGCAAGATACTGGGGCCAC CATTGACTTGGTGATCGGCTATGATCCGCCTTCTGCTCCACATCCAAATGACCTGAGCGGGCCCAGCGTG CCAGGCATGGGAGGAGATGGGGAAGAAGATGAAGGTGATGAAGACAGGTTGGACAATGCAGTCAGGGGCC CTGGGCCCAAGGGGCCAGTTGGGACGGTGTCGGAAGCTCAGCTTGCTCGGAGGCTCACCAAAGTAAAGAA CAGCCGGCGGATGCTGTCAAATAAGCCACAGGACTTCCAGATCCGCGTCCGAGTGATTGAGGGCCGACAG TTAAGTGGTAACAACATAAGGCCTGTGGTCAAAGTTCACGTCTGTGGCCAGACACACCGAACAAGAATCA AGAGAGGAAACAACCCTTTTTTTGATGAGTTGTTTTTCTACAATGTCAACATGACCCCTTCTGAATTGAT GGATGAGATCATCAGCATCCGGGTTTATAATTCTCACTCTCTGCGGGCAGATTGTCTGATGGGGGAATTT AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCATGCTGTCATGAGAAAGTGGCTTCTTCTCAATG ACCCGGAAGATACCAGTTCAGGTTCTAAAGGTTATATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA TGAGCCTCCTCCTGAGAGACGAGATCGTGATAATGACAGTGATGATGTGGAGAGTAATTTGTTACTCCCT GCTGGCATTGCCCTCCGGTGGGTGACCTTCTTGCTGAAAATCTACCGAGCTGAGGACATCCCCCAGATGG ATGATGCCTTCTCACAGACAGTAAAGGAAATATTTGGAGGCAATGCAGATAAGAAAAATCTCGTGGATCC TTTTGTAGAAGTTTCCTTTGCTGGAAAAAAGGTTTGTACAAACATAATTGAGAAAAATGCAAACCCAGAG TGGAATCAGGTCGTCAATCTTCAGATCAAGTTTCCTTCAGTGTGTGAAAAAATAAAACTAACAATATATG ACTGGGACCGTCTTACTAAAAATGATGTAGTTGGAACAACATATCTACACCTCTCTAAAATTGCTGCCTC TGGTGGGGAAGTGGAAGTAAACACAGGAGAAACAGAGGTAGGCTTTGTTCCAACGTTTGGACCTTGTTAC CTGAATCTTTATGGAAGCCCCAGAGAGTACACGGGATTCCCAGACCCCTATGATGAGCTGAATACTGGAA AGGGGGAAGGAGTTGCCTACAGAGGCAGGATCTTGGTTGAATTAGCCACTTTTCTTGAGAAGACACCACC AGATAAAAAGCTTGAGCCCATTTCAAATGATGACCTGCTGGTTGTTGAGAAATACCAGCGAAGGCGGAAG TACAGCCTGTCTGCCGTGTTTCATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAG TCAGCATTGGGAACTATGGCAACAAGTTTGACACCACCTGTAAGCCTTTGGCATCAACAACTCAGTACAG CCGTGCTGTATTTGATGGCAACTACTATTATTACTTGCCTTGGGCCCACACCAAGCCAGTTGTTACCCTG ACTTCATACTGGGAGGATATTAGTCATCGCCTGGATGCGGTGAACACTCTCCTAGCTATGGCAGAACGGC TGCAAACAAATATAGAAGCTCTAAAATCAGGGATACAAGGTAAAATTCCTGCAAACCAGCTGGCTGAATT GTGGCTGAAGCTGATAGATGAAGTTATAGAAGACACGAGATACACGTTGCCTCTCACAGAAGGAAAAGCC AACGTCACAGTTCTCGATACTCAGATCCGAAAGCTGCGGTCCAGGTCTCTCTCCCAAATACATGAGGCGG CTGTGAGGATGAGGTCGGAAGCCACAGATGTGAAGTCCACACTGGCAGAAATTGAGGACTGGCTTGATAA ATTAATGCAGCTGACTGAAGAGCCACAGAACAGCATGCCTGACATCATCATCTGGATGATCCGGGGAGAG AAGAGACTGGCCTATGCACGAATTCCCGCACATCAGGTCTTGTACTCCACCAGTGGTGAGAATGCATCTG GAAAATACTGTGGGAAAACCCAAACCATCTTTCTGAAGTATCCACAGGAGAAAAACAACGGGCCAAAGGT GCCTGTGGAGTTGCGAGTGAACATCTGGCTAGGCTTAAGTGCTGTGGAGAAGAAGTTTAACAGCTTCGCA GAAGGAACTTTCACCGTCTTTGCTGAAATGTATGAAAATCAAGCTCTCATGTTTGGAAAATGGGGTACTT CTGGATTAGTAGGACGTCATAAGTTTTCTGATGTCACAGGAAAAATAAAACTCAAGAGGGAATTTTTTCT GCCTCCAAAAGGCTGGGAATGGGAAGGAGAGTGGATAGTTGATCCTGAAAGAAGCTTGCTGACTGAGGCA GATGCAGGTCACACGGAGTTCACTGATGAAGTCTATCAGAACGAGAGCCGCTACCCCGGGGGCGACTGGA AGCCGGCCGAGGACACCTACACGGATGCGAACGGCGATAAAGCAGCATCACCCAGCGAGTTGACTTGTCC TCCAGGTTGGGAATGGGAAGATGATGCATGGTCTTATGACATAAATCGAGCGGTGGATGAGAAAGGCTGG GAATATGGAATCACCATTCCTCCTGATCATAAGCCCAAATCCTGGGTTGCAGCAGAGAAAATGTACCACA CTCATAGACGGCGAAGGCTGGTCCGAAAACGCAAGAAAGATTTAACACAGACTGCTTCAAGCACCGCAAG GGCCATGGAGGAATTGCAAGACCAAGAGGGCTGGGAATATGCTTCTCTAATTGGCTGGAAATTTCACTGG AAACAACGTAGTTCAGATACCTTCCGCCGCAGACGCTGGAGGAGAAAAATGGCTCCTTCAGAAACACATG GTGCAGCTGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCAGACACTACCGAAGATGGGGATGAGAAGAG CCTGGAGAAACAGAAGCACAGTGCCACCACTGTGTTCGGAGCAAACACCCCCATTGTTTCCTGCAATTTT GACAGAGTCTACATCTACCATCTGCGCTGCTATGTCTATCAAGCCAGAAACCTCTTGGCTTTAGATAAGG ATAGCTTTTCAGATCCATATGCTCATATCTGTTTCCTCCATCGGAGCAAAACCACTGAGATCATCCATTC AACCCTGAATCCCACGTGGGACCAAACAATTATATTCGATGAAGTTGAAATCTATGGGGAACCCCAAACA GTTCTACAGAATCCACCCAAAGTTATCATGGAACTTTTTGACAATGACCAAGTGGGCAAAGATGAATTTT TAGGACGAAGCATTTTCTCTCCTGTGGTGAAACTGAACTCAGAAATGGACATCACACCCAAACTTCTCTG GCACCCAGTAATGAATGGAGACAAAGCCTGCGGGGATGTTCTTGTAACTGCAGAGCTGATTCTGAGGGGC AAGGATGGCTCCAACCTTCCCATTCTTCCCCCTCAAAGGGCGCCAAATCTATACATGGTCCCCCAGGGGA TCAGGCCTGTGGTCCAGCTCACTGCCATTGAGATTCTAGCTTGGGGCTTAAGAAATATGAAAAACTTCCA GATGGCTTCTATCACATCCCCCAGTCTTGTTGTGGAGTGTGGAGGAGAAAGGGTGGAATCGGTGGTGATC AAAAACCTTAAGAAGACACCCAACTTTCCAAGTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCAAGGAGG AATTGTACATGCCCCCACTGGTGATCAAGGTCATCGACCACAGGCAGTTTGGGCGGAAGCCTGTCGTCGG CCAGTGCACCATCGAGCGCCTGGACCGCTTTCGCTGTGACCCTTATGCAGGGAAAGAGGACATCGTCCCA CAGCTCAAAGCCTCCCTTCTGTCTGCCCCACCATGCCGGGACATCGTTATCGAAATGGAAGACACCAAAC CATTACTGGCTTCTAAGCTGACAGAAAAGGAGGAAGAAATCGTGGACTGGTGGAGTAAATTTTATGCTTC CTCAGGGGAACATGAAAAATGCGGACAGTATATTCAGAAAGGCTATTCCAAGCTCAAGATATATAATTGT GAACTAGAAAATGTAGCAGAATTTGAGGGCCTGACAGACTTCTCAGATACGTTCAAGTTGTACCGAGGCA AGTCGGATGAAAATGAAGATCCTTCTGTGGTTGGAGAGTTTAAGGGCTCCTTTCGGATCTACCCTCTGCC GGATGACCCCAGCGTGCCAGCCCCTCCCAGACAGTTTCGGGAATTACCTGACAGCGTCCCACAGGAATGC ACGGTTAGGATTTACATTGTTCGAGGCTTAGAGCTCCAGCCCCAGGACAACAATGGCCTGTGTGACCCTT ACATAAAAATAACACTGGGCAAAAAAGTCATTGAAGACCGAGATCACTACATTCCCAACACTCTCAACCC AGTCTTTGGCAGGATGTACGAACTGAGCTGCTACTTACCTCAAGAAAAAGACCTGAAAATTTCTGTCTAT GATTATGACACCTTTACCCGGGATGAAAAAGTAGGAGAAACAATTATTGATCTGGAAAACCGATTCCTTT CCCGCTTTGGGTCCCACTGCGGCATACCAGAGGAGTACTGTGTTTCTGGAGTCAATACCTGGCGAGATCA ACTGAGACCAACACAGCTGCTTCAAAATGTCGCCAGATTCAAAGGCTTCCCACAACCCATCCTTTCCGAA GATGGGAGTAGAATCAGATATGGAGGACGAGACTACAGCTTGGATGAATTTGAAGCCAACAAAATCCTGC ACCAGCACCTCGGGGCCCCTGAAGAGCGGCTTGCTCTTCACATCCTCAGGACTCAGGGGCTGGTCCCTGA GCACGTGGAAACAAGGACTTTGCACAGCACCTTCCAGCCCAACATTTCCCAGGGAAAACTTCAGATGTGG GTGGATGTTTTCCCCAAGAGTTTGGGGCCACCAGGCCCTCCTTTCAACATCACACCCCGGAAAGCCAAGA AATACTACCTGCGTGTGATCATCTGGAACACCAAGGACGTTATCTTGGACGAGAAAAGCATCACAGGAGA GGAAATGAGTGACATCTACGTCAAAGGCTGGATTCCTGGCAATGAAGAAAACAAACAGAAAACAGATGTC CATTACAGATCTTTGGATGGTGAAGGGAATTTTAACTGGCGATTTGTTTTCCCGTTTGACTACCTTCCAG CCGAACAACTCTGTATCGTTGCGAAAAAAGAGCATTTCTGGAGTATTGACCAAACGGAATTTCGAATCCC ACCCAGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCTCTGGATGACTACTTGGGTTTCCTAGAA CTTGACTTGCGTCACACGATCATTCCTGCAAAATCACCAGAGAAATGCAGATTGGACATGATTCCGGACC TCAAAGCCATGAACCCCCTTAAAGCCAAGACAGCCTCCCTCTTTGAGCAGAAGTCCATGAAAGGATGGTG GCCATGCTACGCAGAGAAAGATGGCGCCCGCGTAATGGCTGGGAAAGTGGAGATGACATTGGAAATCCTC AACGAGAAGGAGGCCGACGAGAGGCCAGCCGGGAAGGGGCGGGACGAACCCAACATGAACCCCAAGCTGG ACTTACCAAATCGACCAGAAACCTCCTTCCTCTGGTTCACCAACCCATGCAAGACCATGAAGTTCATCGT GTGGCGCCGCTTTAAGTGGGTCATCATCGGCTTGCTGTTCCTGCTTATCCTGCTGCTCTTCGTGGCCGTG CTCCTCTACTCTTTGCCGAACTATTTGTCAATGAAGATTGTAAAGCCAAATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212351 representing NM_133337 Red=Cloning site Green=Tags(s) MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVK DFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSV PGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQ LSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEF KIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLP AGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPE WNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEVNTGETEVGFVPTFGPCY LNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRK YSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTL TSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKA NVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGE KRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFA EGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEA DAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGW EYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHW KQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNF DRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQT VLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRG KDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVI KNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVP QLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNC ELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQEC TVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVY DYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSE DGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMW VDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDV HYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLE LDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEIL NEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAV LLYSLPNYLSMKIVKPNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133337
ORF Size	6144 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133337.3
RefSeq Size	6821 bp
RefSeq ORF	6147 bp
Locus ID	26509
UniProt ID	Q9NZM1
Domains	C2, DysFN, DysFC
Protein Families	Transmembrane
MW	233.5 kDa
Gene Summary	Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008]

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