SIRT4 (NM_012240) Human Tagged ORF Clone
CAT#: RC212226
SIRT4 (Myc-DDK-tagged)-Human sirtuin 4 (SIRT4)
ORF Plasmid: tGFP
"NM_012240" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 6 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SIR2L4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212226 representing NM_012240
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGAGCTTTGCGTTGACTTTCAGGTCAGCAAAAGGCCGTTGGATCGCAAACCCCAGCCAGCCGT GCTCGAAAGCCTCCATTGGGTTATTTGTGCCAGCAAGTCCTCCTCTGGACCCTGAGAAGGTCAAAGAGTT ACAGCGCTTCATCACCCTTTCCAAGAGACTCCTTGTGATGACTGGGGCAGGAATCTCCACCGAATCGGGG ATACCAGACTACAGGTCAGAAAAAGTGGGGCTTTATGCCCGCACTGACCGCAGGCCCATCCAGCATGGTG ATTTTGTCCGGAGTGCCCCAATCCGCCAGCGGTACTGGGCGAGAAACTTCGTAGGCTGGCCTCAATTCTC CTCCCACCAGCCTAACCCTGCACACTGGGCTTTGAGCACCTGGGAGAAACTCGGAAAGCTGTACTGGTTG GTGACCCAAAATGTGGATGCTTTGCACACCAAGGCGGGGAGTCGGCGCCTGACAGAGCTCCACGGATGCA TGGACAGGGTCCTGTGCTTGGATTGTGGGGAACAGACTCCCCGGGGGGTGCTGCAAGAGCGTTTCCAAGT CCTGAACCCCACCTGGAGTGCTGAGGCCCATGGCCTGGCTCCTGATGGTGACGTCTTTCTCTCAGAGGAG CAAGTCCGGAGCTTTCAGGTCCCAACCTGCGTTCAATGTGGAGGCCATCTGAAACCAGATGTCGTTTTCT TCGGGGACACAGTGAACCCTGACAAGGTTGATTTTGTGCACAAGCGTGTAAAAGAAGCCGACTCCCTCTT GGTGGTGGGATCATCCTTGCAGGTATACTCTGGTTACAGGTTTATCCTCACTGCCTGGGAGAAGAAGCTC CCGATTGCAATACTGAACATTGGGCCCACACGGTCGGATGACTTGGCGTGTCTGAAACTGAATTCTCGTT GTGGAGAGTTGCTGCCTTTGATAGACCCATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212226 representing NM_012240
Red=Cloning site Green=Tags(s) MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESG IPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWL VTQNVDALHTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEE QVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKL PIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012240 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012240.2 |
RefSeq Size | 1174 bp |
RefSeq ORF | 945 bp |
Locus ID | 23409 |
UniProt ID | Q9Y6E7 |
Domains | SIR2 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 35 kDa |
Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] |
Citations (6)
The use of this cDNA Clones has been cited in the following citations: |
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SIRT4 prevents excitotoxicity via modulating glutamate metabolism in glioma cells
,Yalçın, GD;Colak, M;,
Hum Exp Toxicol
,PubMed ID 32081049
[SIRT4]
|
SIRT4 enhances the sensitivity of ER-positive breast cancer to tamoxifen by inhibiting the IL-6/STAT3 signal pathway
,Xing, J;Li, J;Fu, L;Gai, J;Guan, J;Li, Q;,
Cancer Med
,PubMed ID 31573734
[SIRT4]
|
SIRT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion
,Sun, H;Huang, D;Liu, G;Jian, F;Zhu, J;Zhang, L;,
Onco Targets Ther
,PubMed ID 30022839
[SIRT4]
|
SIRT4 interacts with OPA1 and regulates mitochondrial quality control and mitophagy
,Lang, A;Anand, R;Altinoluk-Hambüchen, S;Ezzahoini, H;Stefanski, A;Iram, A;Bergmann, L;Urbach, J;Böhler, P;Hänsel, J;Franke, M;Stühler, K;Krutmann, J;Scheller, J;Stork, B;Reichert, AS;Piekorz, RP;,
Aging (Albany NY)2017
,PubMed ID 29081403
[SIRT4]
|
SIRT4 inhibits malignancy progression of NSCLCs, through mitochondrial dynamics mediated by the ERK-Drp1 pathway
,Fu, L;Dong, Q;He, J;Wang, X;Xing, J;Wang, E;Qiu, X;Li, Q;,
Oncogene
,PubMed ID 27941873
[SIRT4]
|
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity
,null,
The Journal of Cell Biology
,PubMed ID 25023514
[SIRT4]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212226L1 | Lenti ORF clone of Human sirtuin 4 (SIRT4), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC212226L2 | Lenti ORF clone of Human sirtuin 4 (SIRT4), mGFP tagged |
CNY 5,890.00 |
|
RC212226L3 | Lenti ORF clone of Human sirtuin 4 (SIRT4), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC212226L4 | Lenti ORF clone of Human sirtuin 4 (SIRT4), mGFP tagged |
CNY 4,800.00 |
|
RG212226 | SIRT4 (tGFP-tagged) - Human sirtuin 4 (SIRT4) |
CNY 4,000.00 |
|
SC310543 | SIRT4 (untagged)-Human sirtuin 4 (SIRT4) |
CNY 2,400.00 |