PALM2AKAP2 (NM_053016) Human Tagged ORF Clone

CAT#: RC211908

  • TrueORF®

PALM2 (Myc-DDK-tagged)-Human paralemmin 2 (PALM2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_053016" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal PALM2 Antibody
    • 100 ug

CNY 5,302.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AKAP-2; AKAP-KL; AKAP2; AKAPKL; MISP2; PALM2; PALM2-AKAP2; PRKA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211908 representing NM_053016
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCGGAATTGCACAAGGAAAGGCTGCAAGCCATAGCAGAAAAAAGAAAGAGGCAGACTGAAA
TAGAAGGCAAGCGACAACAGCTTGACGAGCAGATACTTCTGCTGCAGCATTCCAAGTCCAAAGTGCTTCG
GGAGAAATGGCTGCTGCAGGGCATACCCGCTGGAACTGCCGAAGAGGAGGAAGCCAGGAGGCGGCAGTCT
GAAGAGGATGAGTTCAGAGTCAAGCAACTTGAAGATAACATTCAGAGGCTGGAGCAAGAAATACAAACGC
TAGAAAGTGAAGAGTCCCAGATATCTGCCAAAGAGCAAATCATCCTAGAGAAACTGAAGGAAACAGAAAA
ATCCTTCAAGGACTTTCAGAAGGGTTTCTCCAGTACGGATGGAGATGCAGTAAATTACATTTCCTCCCAG
CTTCCCGACCTGCCAATCCTCTGTTCACGAACAGCAGAACCATCACCTGGGCAGGACGGGACCAGCAGAG
CGGCTGCTGTGTACGCCATGGAAATTAATGTGGAGAAAGACAAACAAACAGGAGAGACCAAGATTCTCTC
TACATCTACCATTGGCCCAGAGGGGGTCCATCAGAAAGGAGTCAAAGTCTATGATGATGGTACCAAAGTA
GTGTATGAGGTGCGCTCAGGAGGCACCGTAGTAGAAAATGGAGTGCACAAATTAAGCACAAAGGATGTAG
AAGAGCTTATTCAGAAGGCTGGACAATCAAGCTTAGGAGGAGGGCACGTGTCTGAAAGGACTGTGATTGC
AGATGGGAGCCTCAGCCATCCCAAGGAACACATGCTCTGCAAAGAAGCTAAGTTAGAAATGGTACATAAG
TCTAGGAAAGACCATTCTTCCGGGAACCCAGGGCAGCAGGCCCAAGCCCCCAGCGCTGCAGGGCCGGAGG
CAAACTTGGATCAGCCCGTCACCATGATTTTTATGGGCTACCAAAATATCGAGGATGAAGAGGAGACGAA
AAAGGTGCTAGGCTATGATGAAACCATCAAGGCTGAATTGGTCCTCATTGATGAAGATGATGAGAAGTCA
TTGAGGGAGAAGACAGTGACGGACGTGTCCACTATTGACGGGAACGCGGCTGAGCTTGTGTCCGGGAGGC
CGGTCTCAGACACCACAGAGCCCTCATCCCCAGAAGGGAAGGAAGAGAGCCTAGCTACAGAGCCAGCCCC
AGGTACCCAAAAGAAAAAGCGCTGTCAATGCTGTGTTGTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211908 representing NM_053016
Red=Cloning site Green=Tags(s)

MAEAELHKERLQAIAEKRKRQTEIEGKRQQLDEQILLLQHSKSKVLREKWLLQGIPAGTAEEEEARRRQS
EEDEFRVKQLEDNIQRLEQEIQTLESEESQISAKEQIILEKLKETEKSFKDFQKGFSSTDGDAVNYISSQ
LPDLPILCSRTAEPSPGQDGTSRAAAVYAMEINVEKDKQTGETKILSTSTIGPEGVHQKGVKVYDDGTKV
VYEVRSGGTVVENGVHKLSTKDVEELIQKAGQSSLGGGHVSERTVIADGSLSHPKEHMLCKEAKLEMVHK
SRKDHSSGNPGQQAQAPSAAGPEANLDQPVTMIFMGYQNIEDEEETKKVLGYDETIKAELVLIDEDDEKS
LREKTVTDVSTIDGNAAELVSGRPVSDTTEPSSPEGKEESLATEPAPGTQKKKRCQCCVVM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053016
ORF Size 1233 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053016.6
RefSeq Size 9363 bp
RefSeq ORF 1236 bp
Locus ID 445815
UniProt ID Q8IXS6
Domains Paralemmin
MW 45.3 kDa
Gene Summary This gene belongs to the paralemmin downstream gene (PDG) family defined in PMID:22855693. Paralemmin downstream genes may have evolved contiguously with the paralemmin genes and are associated with other paralemmin paralogs in humans and several other taxa. The gene encodes three distinct protein isoforms, the PALM2 isoform, the AKAP2 isoform and the PALM2-AKAP2 isoform. The biological significance of the PALM2-AKAP2 isoforms is yet unknown. Earlier, PALM2 and AKAP2 were annotated as separate genes and PALM2-AKAP2 was annotated as a readthrough gene. [provided by RefSeq, May 2019]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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