Glucuronic Acid Epimerase (GLCE) (NM_015554) Human Tagged ORF Clone
CAT#: RC211866
- TrueORF®
GLCE (Myc-DDK-tagged)-Human glucuronic acid epimerase (GLCE)
ORF Plasmid: tGFP
"NM_015554" in other vectors (6)
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CNY 4,592.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HSEPI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211866 representing NM_015554
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTGCTTGGCAGCTCGGGTCAACTATAAGACTTTGATTATTATCTGCGCACTCTTCACTTTGGTCA CAGTACTTTTGTGGAATAAGTGTTCCAGTGACAAAGCAATCCAGTTTCCACGGCGTTCGAGTAGTGGCTT CAGAGTGGATGGGTTTGAAAAAAGAGCAGCAGCATCTGAGAGTAACAACTATATGAACCACGTGGCCAAA CAACAGTCTGAGGAAGCATTCCCTCAGGAACAGCAGAAAGCACCCCCTGTTGTTGGGGGCTTCAATAGCA ATGTGGGAAGTAAGGTGTTAGGGCTCAAATATGAAGAAATTGACTGTCTCATAAATGATGAACACACAAT TAAAGGGAGACGAGAGGGGAACGAAGTCTTTCTTCCATTCACTTGGGTTGAGAAATATTTTGATGTTTAT GGAAAGGTGGTTCAGTATGATGGCTATGATCGGTTTGAATTCTCTCATAGCTATTCCAAAGTCTATGCAC AGAGAGCCCCCTATCACCCCGATGGTGTGTTTATGTCTTTTGAAGGCTACAATGTGGAAGTCCGAGACAG AGTCAAGTGCATAAGTGGGGTTGAAGGTGTGCCATTATCTACACAATGGGGACCTCAAGGCTATTTCTAT CCAATCCAGATTGCACAGTATGGATTAAGTCATTACAGCAAGAATCTAACTGAGAAACCTCCTCACATAG AGGTATATGAAACAGCAGAAGACAGAGACAAAAACAAGCCTAATGACTGGACTGTGCCAAAGGGCTGCTT TATGGCGAATGTGGCTGATAAGTCTAGATTCACCAATGTCAAACAGTTTATTGCACCAGAAACCAGTGAA GGTGTATCCTTGCAACTGGGAAACACAAAAGATTTTATTATTTCATTTGACCTCAAGTTCTTGACAAATG GAAGTGTGTCCGTGGTTCTAGAGACCACAGAAAAGAATCAGCTCTTCACTATACATTATGTCTCAAATGC TCAGCTAATTGCTTTTAAAGAAAGAGATATATACTATGGCATTGGGCCCAGAACTTCATGGAGCACAGTT ACCAGGGACCTGGTCACTGACCTCAGGAAAGGAGTGGGTCTTTCAAACACAAAAGCTGTCAAGCCAACCA AAATAATGCCCAAGAAGGTGGTTAGGTTGATTGCAAAAGGTAAGGGATTCCTCGACAACATTACCATCTC TACCACAGCCCACATGGCTGCATTTTTTGCTGCTAGTGATTGGCTAGTAAGGAACCAGGATGAGAAAGGT GGCTGGCCAATTATGGTGACCCGTAAGTTAGGGGAAGGGTTCAAGTCTTTAGAGCCAGGATGGTATTCTG CCATGGCCCAAGGGCAAGCCATTTCTACATTAGTCAGGGCCTATCTGTTAACAAAAGACCATATATTCCT CAATTCAGCTTTAAGGGCAACAGCCCCTTATAAGTTTCTATCTGAGCAGCATGGAGTTAAAGCTGTGTTT ATGAATAAACATGACTGGTATGAAGAATATCCAACCACACCTAGCTCTTTTGTTTTAAATGGCTTTATGT ATTCTTTAATTGGGCTGTATGACTTAAAAGAAACTGCAGGGGAAAAACTCGGAAAAGAAGCAAGGTCCTT GTATGAGCGTGGCATGGAATCTCTTAAAGCCATGCTGCCCTTGTATGACACTGGCTCAGGAACCATCTAT GACCTCCGTCACTTCATGCTTGGCATCGCTCCTAACCTGGCTCGCTGGGACTATCATACCACCCACATCA ATCAGTTGCAGCTACTCAGTACCATTGATGAGTCCCCAGTCTTCAAAGAATTTGTCAAGAGGTGGAAAAG CTACCTTAAAGGCAGCAGGGCAAAGCACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211866 representing NM_015554
Red=Cloning site Green=Tags(s) MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAK QQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVY GKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFY PIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSE GVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTV TRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKG GWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVF MNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIY DLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015554 |
ORF Size | 1851 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015554.3 |
RefSeq Size | 5043 bp |
RefSeq ORF | 1854 bp |
Locus ID | 26035 |
UniProt ID | O94923 |
Protein Families | Transmembrane |
Protein Pathways | Heparan sulfate biosynthesis, Metabolic pathways |
MW | 69.9 kDa |
Gene Summary | Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Enzyme overexpression?-?an exercise toward understanding regulation of heparan sulfate biosynthesis
,Fang, J;Song, T;Lindahl, U;Li, JP;,
Sci Rep
,PubMed ID 27511124
[GLCE]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211866L1 | Lenti ORF clone of Human glucuronic acid epimerase (GLCE), Myc-DDK-tagged |
CNY 6,992.00 |
|
RC211866L2 | Lenti ORF clone of Human glucuronic acid epimerase (GLCE), mGFP tagged |
CNY 6,460.00 |
|
RC211866L3 | Lenti ORF clone of Human glucuronic acid epimerase (GLCE), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC211866L4 | Lenti ORF clone of Human glucuronic acid epimerase (GLCE), mGFP tagged |
CNY 6,460.00 |
|
RG211866 | GLCE (tGFP-tagged) - Human glucuronic acid epimerase (GLCE) |
CNY 5,040.00 |
|
SC124778 | GLCE (untagged)-Human glucuronic acid epimerase (GLCE) |
CNY 4,600.00 |
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