BAI3 (ADGRB3) (NM_001704) Human Tagged ORF Clone

CAT#: RC211798

BAI3 (Myc-DDK-tagged)-Human brain-specific angiogenesis inhibitor 3 (BAI3)

ORF Plasmid: DDK tGFP



  "NM_001704" in other vectors (6)

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CNY 10,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


BAI3 (C-Terminus) rabbit polyclonal antibody, Immunoaffinity purified
    • 50 ug

CNY 5,654.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BAI3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211798 representing NM_001704
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCTGTTCGTAACCTGCTGATTTATATATTTTCCACCTATCTCCTGGTTATGTTTGGATTTAATG
CTGCCCAAGACTTCTGGTGTTCAACTTTGGTGAAGGGAGTCATTTATGGATCGTATTCTGTAAGTGAAAT
GTTTCCTAAAAACTTTACAAACTGCACTTGGACGCTGGAAAATCCAGATCCAACCAAATATAGCATTTAC
CTGAAATTTTCCAAAAAGGACCTTAGCTGCTCTAACTTTTCACTCCTGGCTTATCAGTTTGATCATTTTT
CCCATGAAAAAATAAAGGATCTTTTAAGAAAGAATCATTCTATAATGCAACTCTGCAATTCCAAGAATGC
TTTCGTTTTTCTACAGTATGATAAAAATTTTATTCAAATACGTCGAGTATTTCCAACTAATTTCCCAGGA
TTACAGAAAAAAGGGGAAGAAGATCAGAAATCTTTTTTTGAGTTTTTGGTATTGAACAAGGTCAGCCCAA
GCCAGTTTGGTTGCCATGTATTATGTACTTGGTTGGAGAGCTGCTTAAAATCAGAAAATGGGAGAACAGA
ATCATGTGGGATCATGTATACAAAATGCACCTGCCCTCAGCATTTGGGAGAGTGGGGGATCGACGACCAG
TCGCTGATTTTGTTAAATAACGTGGTGTTACCCCTGAATGAGCAGACAGAGGGCTGCCTGACCCAGGAGC
TGCAAACCACCCAAGTCTGCAATCTTACCAGGGAGGCCAAGCGACCACCCAAAGAAGAATTTGGAATGAT
GGGAGATCATACAATTAAAAGTCAGCGACCTCGATCTGTTCATGAAAAAAGGGTCCCTCAGGAACAAGCT
GATGCTGCTAAATTTATGGCACAAACTGGTGAATCTGGTGTGGAAGAGTGGTCCCAGTGGAGCACATGTT
CGGTTACTTGTGGTCAAGGGTCGCAGGTGCGAACCAGAACTTGTGTATCACCTTACGGGACACACTGCAG
CGGCCCATTAAGAGAATCAAGGGTTTGCAATAACACTGCCCTCTGTCCAGTACACGGAGTATGGGAGGAA
TGGTCACCATGGAGTTTATGTTCATTTACATGTGGTCGAGGCCAAAGAACAAGAACAAGGTCATGCACAC
CTCCTCAGTATGGAGGAAGGCCGTGTGAAGGACCTGAAACACATCATAAGCCTTGTAATATTGCTCTTTG
CCCAGTTGATGGACAGTGGCAAGAGTGGAGTTCGTGGAGCCAGTGCTCAGTAACGTGCTCGAATGGGACT
CAGCAGAGAAGCCGGCAGTGCACTGCAGCTGCCCATGGAGGCTCCGAATGCAGAGGGCCATGGGCAGAAA
GCAGAGAGTGCTATAACCCTGAATGTACAGCCAATGGTCAATGGAATCAGTGGGGTCATTGGAGTGGTTG
TTCCAAGTCCTGTGATGGCGGCTGGGAAAGGCGAATAAGGACCTGTCAGGGTGCAGTGATAACAGGGCAG
CAATGTGAAGGAACGGGCGAAGAAGTGAGAAGATGCAGTGAGCAGCGATGCCCTGCACCTTATGAAATAT
GCCCTGAGGATTATCTGATGTCGATGGTGTGGAAAAGAACTCCAGCAGGCGACTTGGCATTCAATCAATG
TCCCCTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTTCTGGGAA
CAGCCGAGCTTTGCAAGATGCATATCAAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTG
CTAAGGGGCAGCGAATGCTGGCAGGTGATGGAATGTCCCAGGTGACCAAGACACTGTTGGATTTAACTCA
GAGAAAAAATTTCTATGCAGGCGATCTTCTGATGTCTGTGGAGATCCTGAGAAATGTGACAGACACATTT
AAAAGGGCAAGTTACATCCCTGCATCTGATGGTGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTAG
ATGAAGAAAACAAGGAAAAATGGGAAGATGCACAACAGATTTATCCAGGGTCAATAGAGTTAATGCAGGT
GATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAGAATTCATACTTAATGACTGGA
AATGTAGTGGCTAGTATTCAGAAGCTTCCTGCAGCCTCTGTTCTAACAGACATCAACTTTCCAATGAAAG
GACGGAAGGGAATGGTTGACTGGGCAAGAAACTCAGAAGATAGGGTAGTAATTCCAAAAAGCATTTTCAC
TCCGGTGTCATCAAAAGAATTAGATGAATCATCTGTATTTGTTCTTGGCGCAGTCCTATACAAAAACTTA
GATCTAATTTTGCCCACTTTGAGAAATTATACTGTCATTAATTCCAAAATCATCGTGGTCACAATAAGGC
CTGAACCCAAAACAACCGATTCGTTTCTGGAGATAGAACTAGCTCATTTGGCTAATGGTACTTTGAATCC
CTATTGTGTATTGTGGGATGACTCCAAAACGAACGAGTCTTTGGGAACGTGGTCCACCCAGGGATGTAAA
ACTGTGCTTACCGATGCATCCCATACGAAATGCTTATGTGATCGTCTCTCTACCTTCGCCATTTTGGCTC
AGCAACCTAGAGAAATAATCATGGAATCCTCTGGCACACCTTCAGTTACCCTAATAGTAGGCAGTGGTCT
TTCTTGCTTGGCCTTGATTACCCTAGCAGTTGTCTATGCAGCATTATGGAGGTACATACGCTCTGAGAGA
TCCATAATACTAATTAACTTCTGCCTGTCTATCATCTCATCCAATATCCTCATACTGGTTGGACAGACTC
AGACACATAATAAGAGTATCTGCACAACCACCACTGCATTTTTGCACTTTTTCTTCCTGGCTTCATTCTG
TTGGGTTTTGACTGAGGCGTGGCAATCATATATGGCTGTAACTGGAAAAATTAGGACACGGCTTATAAGA
AAACGCTTTTTGTGCCTTGGATGGGGTTTACCAGCATTAGTAGTGGCCACATCAGTAGGCTTCACCAGAA
CAAAAGGATATGGCACTGATCACTACTGCTGGCTCTCTCTTGAAGGAGGACTACTCTATGCTTTTGTGGG
ACCTGCAGCCGCTGTTGTCCTGGTCAACATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGA
GATGGAATCCTAGATAAAAAGCTCAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTTTGACGC
TCAAATGTGCCAAGTGTGGAGTAGTTTCAACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGC
GTCTCTTTGGAGCTCCTGTGTGGTGTTGCCCCTTCTGGCTTTGACGTGGATGTCTGCGGTTCTGGCCATG
ACAGATAAACGCTCCATATTGTTTCAAATACTTTTTGCTGTGTTTGATTCATTGCAAGGCTTTGTTATAG
TCATGGTCCACTGCATTCTTCGGAGAGAGGTTCAGGATGCATTTAGATGCCGATTGAGAAACTGTCAGGA
TCCCATCAATGCAGATTCTTCGAGTTCGTTTCCTAATGGGCATGCTCAAATCATGACAGACTTTGAAAAG
GATGTAGACATTGCCTGTCGATCAGTTCTTCATAAGGATATTGGTCCTTGCCGAGCAGCCACAATAACAG
GAACACTTTCTAGGATTTCTCTAAATGATGATGAAGAAGAAAAGGGAACAAACCCTGAAGGGCTAAGCTA
TTCAACATTGCCTGGAAATGTCATTTCCAAAGTCATCATCCAGCAACCCACAGGTTTGCACATGCCCATG
AGTATGAATGAGCTTAGCAATCCATGTTTGAAAAAAGAAAATAGTGAATTGCGGAGAACTGTGTACTTAT
GTACGGATGATAATTTGAGAGGGGCTGACATGGACATAGTCCATCCTCAAGAAAGAATGATGGAAAGTGA
CTATATTGTGATGCCCAGAAGTTCTGTAAATAACCAGCCTTCAATGAAAGAAGAAAGCAAAATGAATATT
GGCATGGAAACCTTGCCACATGAAAGGCTATTGCACTACAAAGTAAACCCTGAATTCAATATGAATCCCC
CTGTAATGGACCAGTTCAATATGAACTTAGAGCAACATCTCGCACCCCAGGAACATATGCAGAATTTGCC
CTTTGAACCTCGCACAGCTGTGAAGAATTTCATGGCCTCTGAGTTGGATGATAATGCAGGACTATCAAGA
AGTGAAACTGGATCAACGATATCAATGAGTTCTTTAGAGAGAAGAAAATCACGATATTCAGACCTTGACT
TTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACTATTTCAAGAACTAAATCAGAAATTTCAAAC
TTTGGACAGATTTCGGGATATACCAAATACAAGCAGTATGGAAAACCCCGCACCAAACAAGAATCCATGG
GACACTTTCAAAAACCCCAGTGAATACCCGCATTACACCACAATCAATGTCTTAGACACAGAGGCAAAGG
ATGCTTTGGAACTGAGGCCAGCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAGAGGGTGA
CTTTCAAACAGAAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211798 representing NM_001704
Red=Cloning site Green=Tags(s)

MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIY
LKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYDKNFIQIRRVFPTNFPG
LQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQ
SLILLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQA
DAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEE
WSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGT
QQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIRTCQGAVITGQ
QCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWE
QPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTF
KRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTG
NVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNL
DLILPTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCK
TVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSER
SIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIR
KRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSR
DGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAM
TDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEK
DVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGLHMPM
SMNELSNPCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNNQPSMKEESKMNI
GMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLEQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSR
SETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPW
DTFKNPSEYPHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001704
ORF Size 4566 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001704.1, NP_001695.1
RefSeq Size 5218 bp
RefSeq ORF 4569 bp
Locus ID 577
UniProt ID O60242
Domains GPS, 7tm_2, tsp_1, HormR
Protein Families Druggable Genome, GPCR, Transmembrane
MW 171.3 kDa
Gene Summary This p53-target gene encodes a brain-specific angiogenesis inhibitor, a seven-span transmembrane protein, and is thought to be a member of the secretin receptor family. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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