MIA3 (NM_198551) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARNT; D320; TANGO; TANGO1; UNQ6077
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC211616 representing NM_198551 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCGCCTGGGCTGCTCGTCTGGCTGCTCGTGCTCCGGCTGCCCTGGCGGGTGCCGGGCCAGC TGGACCCCAGCACTGGCCGGCGGTTCTCGGAGCACAAACTCTGCGCGGACGACGAATGCAGCATGTTAAT GTACCGCGGTGAGGCTCTTGAAGATTTCACAGGCCCGGATTGTCGTTTTGTGAATTTTAAAAAAGGTGAT CCTGTATATGTTTACTATAAACTGGCAAGAGGATGGCCTGAAGTTTGGGCTGGAAGTGTTGGACGCACTT TTGGATATTTTCCAAAAGATTTAATCCAGGTAGTTCATGAATATACCAAAGAAGAGCTACAAGTTCCAAC AGATGAGACGGATTTTGTTTGTTTTGATGGAGGAAGAGATGATTTTCATAATTATAATGTAGAAGAACTT TTAGGGTTTTTGGAACTGTACAATTCTGCAGCTACAGATTCTGAGAAAGCTGTAGAAAAAACTTTACAGG ATATGGAAAAAAACCCTGAATTATCTAAGGAAAGGGAACCTGAACCTGAACCAGTAGAAGCCAACTCAGA GGAAAGTGATAGTGTATTCTCAGAAAACACTGAGGATCTTCAGGAACAGTTTACAACTCAGAAGCACCAC TCCCATGCAAACAGCCAAGCAAATCATGCTCAGGGAGAGCAGGCTTCATTTGAATCTTTTGAAGAAATGC TGCAAGATAAACTAAAAGTGCCAGAAAGTGAAAACAACAAAACCAGCAATAGTTCTCAGGTCTCAAATGA ACAGGATAAGATTGATGCCTATAAACTTTTGAAAAAAGAAATGACTCTAGACTTGAAAACCAAATTTGGC TCAACAGCTGATGCACTTGTATCTGATGATGAGACAACCAGACTCGTTACTTCATTAGAAGATGATTTTG ATGAGGAATTGGATACTGAGTATTATGCAGTTGGAAAGGAAGATGAGGAGAACCAAGAAGACTTTGATGA GTTGCCATTACTTACCTTTACAGATGGGGAAGATATGAAAACTCCAGCAAAGTCTGGCGTTGAGAAATAT CCAACAGATAAAGAGCAGAATTCAAATGAAGAGGACAAGGTTCAGCTAACTGTGCCCCCTGGCATCAAAA ATGATGATAAAAATATACTAACAACCTGGGGGGACACTATCTTCTCTATTGTCACAGGAGGTGAAGAAAC AAGAGATACGATGGATTTAGAGAGCTCTAGTTCAGAGGAAGAAAAAGAAGATGATGATGATGCATTAGTC CCAGATAGCAAACAGGGGAAACCACAGTCAGCAACAGATTATAGTGACCCTGACAATGTAGATGATGGTC TTTTTATTGTAGACATTCCTAAAACAAATAATGACAAAGAAGTAAACGCAGAACATCACATTAAAGGAAA AGGGAGGGGAGTTCAGGAATCCAAGAGGGGCCTGGTACAAGATAAGACAGAATTAGAGGATGAAAATCAA GAAGGCATGACTGTGCACAGTTCTGTTCACAGCAATAACCTCAACTCTATGCCAGCTGCTGAAAAGGGTA AAGACACATTAAAATCAGCTTATGATGATACAGAAAATGACCTAAAAGGAGCAGCTATTCATATCTCAAA AGGAATGCTCCACGAAGAAAAGCCTGGAGAGCAGATTTTGGAAGGTGGCTCAGAGAGTGAATCTGCACAG AAAGCTGCAGGGAATCAAATGAATGACAGAAAGATTCAACAGGAATCCCTGGGTAGTGCACCACTCATGG GAGATGACCACCCTAACGCATCCAGAGACAGTGTGGAGGGAGACGCTTTGGTAAATGGGGCCAAACTGCA CACGCTTTCAGTGGAGCATCAACGTGAGGAATTGAAAGAGGAATTAGTTCTTAAAACTCAAAACCAACCT AGATTCTCCTCTCCAGATGAGATTGATTTGCCCAGAGAACTGGAAGACGAGGTTCCCATTCTGGGAAGAA ATCTTCCCTGGCAACAAGAAAGAGATGTGGCTGCCACAGCCAGTAAGCAAATGAGTGAGAAGATAAGGCT CTCTGAGGGAGAAGCCAAAGAGGACTCCTTGGATGAAGAGTTTTTTCATCACAAGGCAATGCAGGGCACA GAGGTAGGACAGACAGACCAAACTGACAGCACAGGAGGACCAGCTTTCCTTTCTAAAGTAGAAGAGGATG ATTATCCCTCTGAAGAACTACTAGAGGATGAAAACGCTATAAATGCAAAACGGTCTAAAGAAAAAAACCC TGGGAATCAGGGCAGGCAGTTTGATGTTAATCTGCAAGTCCCTGACAGAGCAGTTTTAGGGACCATTCAT CCAGATCCAGAAATTGAAGAAAGCAAGCAAGAAACTAGTATGATTTTGGATAGCGAAAAAACAAGTGAGA CTGCTGCCAAAGGGGTCAACACAGGAGGCAGGGAACCAAATACAATGGTGGAAAAAGAACGCCCTCTGGC AGATAAGAAAGCACAGAGACCATTTGAACGAAGTGACTTTTCTGACAGCATAAAAATTCAGACTCCAGAA TTAGGTGAAGTGTTTCAGAATAAAGATTCTGATTATCTGAAGAACGACAACCCTGAGGAACATCTGAAGA CCTCAGGGCTTGCAGGGGAGCCTGAGGGAGAACTCTCAAAAGAGGACCATGAGAACACAGAGAAGTACAT GGGCACAGAAAGCCAGGGGTCTGCTGCTGCAGAACCTGAAGATGACTCGTTCCACTGGACTCCACATACA AGTGTAGAGCCAGGGCATAGTGACAAGAGGGAGGACTTACTTATCATAAGCAGCTTCTTTAAAGAACAAC AGTCTTTGCAGCGGTTCCAGAAGTACTTTAATGTCCATGAGCTGGAAGCCTTGCTACAAGAAATGTCATC AAAACTGAAGTCAGCGCAGCAGGAGAGCCTGCCCTATAATATGGAAAAAGTCCTAGATAAGGTCTTCCGT GCTTCTGAGTCACAAATTCTGAGCATAGCAGAAAAAATGCTTGATACTCGTGTGGCTGAAAATAGAGATC TGGGAATGAACGAAAATAACATATTTGAAGAGGCTGCAGTGCTTGATGACATTCAAGACCTCATCTATTT TGTCAGGTACAAGCACTCCACAGCAGAGGAGACAGCCACACTGGTGATGGCACCACCTCTAGAGGAAGGC TTGGGTGGAGCAATGGAAGAGATGCAACCACTGCATGAAGATAATTTCTCACGAGAGAAGACAGCAGAAC TTAATGTGCAGGTTCCTGAAGAACCCACCCACTTGGACCAACGTGTGATTGGGGACACTCATGCCTCAGA AGTGTCACAGAAGCCAAATACTGAGAAAGACCTGGACCCAGGGCCAGTTACAACAGAAGACACTCCTATG GATGCTATTGATGCAAACAAGCAACCAGAGACAGCCGCCGAAGAGCCGGCAAGTGTCACACCTTTGGAAA ACGCAATCCTTCTAATATATTCATTCATGTTTTATTTAACTAAGTCGCTAGTTGCTACATTGCCTGATGA TGTTCAGCCTGGGCCTGATTTTTATGGACTGCCATGGAAACCTGTATTTATCACTGCCTTCTTGGGAATT GCTTCGTTTGCCATTTTCTTATGGAGAACTGTCCTTGTTGTGAAGGATAGAGTATATCAAGTCACGGAAC AGCAAATTTCTGAGAAGTTGAAGACTATCATGAAAGAAAATACAGAACTTGTACAAAAATTGTCAAATTA TGAACAGAAGATCAAGGAATCAAAGAAACATGTTCAGGAAACCAGGAAACAAAATATGATTCTCTCTGAT GAAGCAATTAAATATAAGGATAAAATCAAGACACTTGAAAAAAATCAGGAAATTCTGGATGACACAGCTA AAAATCTTCGTGTTATGCTAGAATCTGAGAGAGAACAGAATGTCAAGAATCAGGACTTGATATCAGAAAA CAAGAAATCTATAGAGAAGTTAAAGGATGTTATTTCAATGAATGCCTCAGAATTTTCAGAGGTTCAGATT GCACTTAATGAAGCTAAGCTTAGTGAAGAGAAGGTGAAGTCTGAATGCCATCGGGTTCAAGAAGAAAATG CTAGGCTTAAGAAGAAAAAAGAGCAGTTGCAGCAGGAAATCGAAGACTGGAGTAAATTACATGCTGAGCT CAGTGAGCAAATCAAATCATTTGAGAAGTCTCAGAAAGATTTGGAAGTAGCTCTTACTCACAAGGATGAT AATATTAATGCTTTGACTAACTGCATTACACAGTTGAATCTGTTAGAGTGTGAATCTGAATCTGAGGGTC AAAATAAAGGTGGAAATGATTCAGATGAATTAGCAAATGGAGAAGTGGGAGGTGACCGGAATGAGAAGAT GAAAAATCAAATTAAGCAGATGATGGATGTCTCTCGGACACAGACTGCAATATCGGTAGTTGAAGAGGAT CTAAAGCTTTTACAGCTTAAGCTAAGAGCCTCCGTGTCCACTAAATGTAACCTGGAAGACCAGGTAAAGA AATTGGAAGATGACCGCAACTCACTACAAGCTGCCAAAGCTGGACTGGAAGATGAATGCAAAACCTTGAG GCAGAAAGTGGAGATTCTGAATGAGCTCTATCAGCAGAAGGAGATGGCTTTGCAAAAGAAACTGAGTCAA GAAGAGTATGAACGGCAAGAAAGAGAGCACAGGCTGTCAGCTGCAGATGAAAAGGCAGTTTCGGCTGCAG AGGAAGTAAAAACTTACAAGCGGAGAATTGAAGAAATGGAGGATGAATTACAGAAGACAGAGCGGTCATT TAAAAACCAGATCGCTACCCATGAGAAGAAAGCTCATGAAAACTGGCTCAAAGCTCGTGCTGCAGAAAGA GCTATAGCTGAAGAGAAAAGGGAAGCTGCCAATTTGAGACACAAATTATTAGAATTAACACAAAAGATGG CAATGCTGCAAGAAGAACCTGTGATTGTAAAACCAATGCCAGGAAAACCAAATACACAAAACCCTCCACG GAGAGGTCCTCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGAGAATGCTCCCCTCCA TTGACAGTGGAGCCACCCGTGAGACCTCTCTCTGCTACTCTCAATCGAAGAGATATGCCTAGAAGTGAAT TTGGATCAGTGGACGGGCCTCTACCTCATCCTCGATGGTCAGCTGAGGCATCTGGGAAACCCTCTCCTTC TGATCCAGGATCTGGTACAGCTACCATGATGAACAGCAGCTCAAGAGGCTCTTCCCCTACCAGGGTACTC GATGAAGGCAAGGTTAATATGGCTCCAAAAGGGCCCCCTCCTTTCCCAGGAGTCCCTCTCATGAGCACCC CCATGGGAGGCCCTGTACCACCACCCATTCGATATGGACCACCACCTCAGCTCTGCGGACCTTTTGGGCC TCGGCCACTTCCTCCACCCTTTGGCCCTGGTATGCGTCCACCACTAGGCTTAAGAGAATTTGCACCAGGC GTTCCACCAGGAAGACGGGACCTGCCTCTCCACCCTCGGGGATTTTTACCTGGACACGCACCATTTAGAC CTTTAGGTTCACTTGGCCCAAGAGAGTACTTTATTCCTGGTACCCGATTACCACCCCCAACCCATGGTCC CCAGGAATACCCACCACCACCTGCTGTAAGAGACTTACTGCCGTCAGGCTCTAGAGATGAGCCTCCACCT GCCTCTCAGAGCACTAGCCAGGACTGTTCACAGGCTTTAAAACAGAGCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC211616 representing NM_198551 Red=Cloning site Green=Tags(s) MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGD PVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEEL LGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHH SHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFG STADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKY PTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALV PDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQ EGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQ KAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQP RFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGT EVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIH PDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPE LGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHT SVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFR ASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEG LGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPM DAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGI ASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSD EAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQI ALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDD NINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEED LKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQ EEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAER AIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPP LTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVL DEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPG VPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPP ASQSTSQDCSQALKQSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_198551
ORF Size	5721 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_198551.4
RefSeq Size	8123 bp
RefSeq ORF	5724 bp
Locus ID	375056
UniProt ID	Q5JRA6
MW	213.5 kDa
Gene Summary	Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255, PubMed:19269366). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536).[UniProtKB/Swiss-Prot Function]

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