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CLCN1 (NM_000083) Human Tagged ORF Clone
CAT＃: RC211343
CLCN1 (Myc-DDK-tagged)-Human chloride channel 1, skeletal muscle (CLCN1)
ORF Plasmid: tGFP
"NM_000083" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211343 representing NM_000083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAATCCCGGTCACAGCAGCGTGGGGGTGAACAAAGCTGGTGGGGTAGTGACCCCCAGTACCAGT ATATGCCCTTTGAACACTGCACCAGCTACGGACTGCCCTCTGAGAATGGGGGCCTCCAGCACAGGCTCCG GAAGGATGCAGGCCCCCGCCACAACGTCCACCCCACACAGATTTATGGCCATCACAAAGAACAATTCTCA GACAGGGAGCAGGACATAGGGATGCCCAAGAAGACAGGCTCCAGTTCTACCGTGGACAGCAAGGATGAGG ATCACTATTCTAAATGTCAAGATTGTATCCACCGCCTGGGACAGGTGGTGAGAAGAAAATTAGGGGAAGA CTGGATCTTTCTGGTGCTTCTGGGACTGCTGATGGCTCTGGTCAGCTGGAGCATGGACTACGTCAGTGCC AAAAGCCTTCAGGCCTACAAGTGGTCCTACGCGCAGATGCAGCCCAGCCTTCCTCTGCAGTTCCTGGTCT GGGTCACCTTCCCACTAGTCCTCATCCTCTTCAGCGCCCTCTTCTGCCACCTCATCTCTCCCCAGGCTGT TGGCTCTGGAATCCCCGAAATGAAGACAATACTTCGTGGGGTTGTCCTGAAGGAATACCTCACAATGAAA GCCTTTGTGGCCAAGGTTGTCGCCCTGACTGCGGGCCTGGGCAGTGGCATCCCCGTGGGGAAAGAGGGCC CCTTCGTCCACATTGCCAGCATCTGTGCTGCTGTCCTCAGCAAATTCATGTCTGTGTTCTGCGGGGTATA TGAGCAGCCATACTACTACTCTGATATCCTGACGGTGGGCTGTGCTGTGGGAGTCGGCTGTTGTTTTGGG ACACCACTTGGAGGAGTGCTATTTAGCATCGAGGTCACCTCCACCTACTTTGCTGTTCGGAACTACTGGA GAGGATTCTTTGCAGCCACGTTCAGCGCCTTTGTGTTTCGAGTGCTGGCAGTGTGGAACAAGGATGCTGT CACCATCACTGCTCTGTTCAGAACCAATTTCCGAATGGATTTCCCCTTTGACCTGAAGGAACTACCAGCT TTTGCTGCCATCGGGATTTGCTGTGGGCTCCTGGGAGCTGTATTTGTGTATCTGCATCGCCAAGTCATGC TCGGTGTCCGAAAGCACAAGGCCCTCAGCCAGTTTCTTGCTAAGCACCGCCTGCTGTATCCTGGAATTGT TACCTTTGTCATTGCCTCATTCACCTTCCCACCAGGAATGGGTCAATTCATGGCTGGAGAGTTGATGCCC CGCGAAGCCATCAGTACTTTGTTTGACAACAATACATGGGTGAAACACGCGGGTGATCCTGAGAGCCTGG GCCAGTCAGCTGTGTGGATTCACCCCCGGGTCAACGTTGTCATCATCATCTTTCTCTTCTTCGTCATGAA GTTCTGGATGTCCATCGTGGCCACCACTATGCCCATACCCTGCGGAGGCTTCATGCCTGTGTTTGTGCTA GGAGCTGCATTTGGAAGGCTGGTAGGAGAAATCATGGCCATGCTCTTTCCTGATGGTATTTTGTTTGATG ACATCATCTACAAGATCCTACCTGGGGGCTATGCAGTAATTGGAGCAGCAGCGCTGACTGGTGCCGTTTC CCACACAGTCTCCACAGCTGTGATTTGCTTCGAATTAACGGGTCAGATTGCTCACATCCTGCCCATGATG GTGGCTGTTATCTTGGCCAACATGGTGGCCCAGAGCCTGCAGCCCTCTCTCTATGACAGCATCATCCAGG TCAAGAAGCTACCCTACTTGCCTGACCTTGGCTGGAACCAGCTCAGCAAATATACCATCTTTGTTGAGGA CATCATGGTACGTGATGTGAAGTTTGTTTCAGCTTCTTACACATATGGGGAGTTGCGAACCCTGCTCCAG ACCACCACAGTCAAGACTTTACCACTGGTTGACTCAAAAGATTCAATGATCCTGCTGGGCTCGGTGGAGC GGTCGGAACTGCAGGCCCTCCTGCAGCGCCACCTGTGTCCTGAGCGCAGGCTGCGCGCAGCCCAAGAGAT GGCGCGGAAGTTGTCGGAGCTGCCTTACGACGGGAAGGCGCGGCTGGCTGGGGAGGGGCTCCCCGGCGCG CCTCCAGGCCGGCCCGAGTCCTTCGCCTTTGTGGATGAGGATGAGGACGAAGATCTCTCTGGCAAGAGCG AGCTTCCTCCTTCCCTTGCTCTCCACCCCTCTACTACTGCCCCTCTGTCCCCAGAAGAGCCCAATGGGCC TCTGCCTGGCCACAAACAGCAGCCGGAAGCACCAGAGCCTGCAGGTCAAAGACCCTCCATCTTCCAGTCC CTGCTTCACTGCTTGCTGGGCAGAGCTCGCCCCACAAAGAAGAAAACAACCCAGGATTCCACAGATTTAG TGGATAACATGTCACCTGAAGAGATTGAGGCCTGGGAGCAGGAGCAGCTGAGCCAGCCTGTCTGTTTTGA TTCCTGCTGTATTGACCAGTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTGCACAAGACTCATACCCTG TTTTCACTCCTTGGCCTCCACCTCGCTTACGTGACCAGCATGGGGAAGCTCAGGGGCGTCCTGGCCCTGG AGGAGCTACAGAAGGCCATTGAGGGGCACACCAAGTCTGGGGTGCAGCTCCGCCCTCCCCTTGCCAGCTT CCGGAACACGACTTCAACTCGAAAGAGTACCGGGGCACCTCCATCTTCTGCAGAGAACTGGAACCTGCCT GAGGACAGGCCTGGGGCCACTGGAACAGGGGATGTGATTGCTGCCTCCCCAGAGACCCCTGTGCCATCTC CTTCCCCAGAGCCCCCTCTCTCCCTGGCCCCAGGCAAGGTAGAGGGCGAGTTGGAGGAGCTGGAGCTGGT GGAGAGTCCAGGGCTGGAAGAGGAGCTGGCCGACATCTTGCAGGGCCCCAGCCTGCGATCCACAGACGAG GAGGATGAGGATGAACTGATCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211343 representing NM_000083
Red=Cloning site Green=Tags(s) MEQSRSQQRGGEQSWWGSDPQYQYMPFEHCTSYGLPSENGGLQHRLRKDAGPRHNVHPTQIYGHHKEQFS DREQDIGMPKKTGSSSTVDSKDEDHYSKCQDCIHRLGQVVRRKLGEDWIFLVLLGLLMALVSWSMDYVSA KSLQAYKWSYAQMQPSLPLQFLVWVTFPLVLILFSALFCHLISPQAVGSGIPEMKTILRGVVLKEYLTMK AFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSVFCGVYEQPYYYSDILTVGCAVGVGCCFG TPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPA FAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGELMP REAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVL GAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMM VAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQ TTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGA PPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQS LLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTL FSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLP EDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDE EDEDELIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000083 |
| ORF Size | 2964 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000083.3 |
| RefSeq Size | 3093 bp |
| RefSeq ORF | 2967 bp |
| Locus ID | 1180 |
| UniProt ID | P35523 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 108.6 kDa |
| Gene Summary | The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211343L1 | Lenti ORF clone of Human chloride channel 1, skeletal muscle (CLCN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211343L2 | Lenti ORF clone of Human chloride channel 1, skeletal muscle (CLCN1), mGFP tagged |
CNY 5,890.00 |
|
| RC211343L3 | Lenti ORF clone of Human chloride channel 1, skeletal muscle (CLCN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211343L4 | Lenti ORF clone of Human chloride channel 1, skeletal muscle (CLCN1), mGFP tagged |
CNY 5,890.00 |
|
| RG211343 | CLCN1 (tGFP-tagged) - Human chloride channel 1, skeletal muscle (CLCN1) |
CNY 4,370.00 |
|
| SC122070 | CLCN1 (untagged)-Human chloride channel 1, skeletal muscle (CLCN1) |
CNY 7,176.00 |
