AAK1 (NM_014911) Human Tagged Lenti ORF Clone
CAT#: RC211284L1
- LentiORF®
Lenti-ORF clone of AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1)
"NM_014911" in other vectors (6)
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CNY 12,952.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211284).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014911 |
ORF Size | 2880 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014911.3 |
RefSeq Size | 21283 bp |
RefSeq ORF | 2886 bp |
Locus ID | 22848 |
UniProt ID | Q2M2I8 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 103.8 kDa |
Gene Summary | This gene encodes a member of the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The encoded protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. This kinase has been shown to play an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of this kinase are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Aug 2020] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211284 | AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 10,552.00 |
|
RC211284L2 | Lenti-ORF clone of AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
RC211284L3 | Lenti-ORF clone of AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
RC211284L4 | Lenti-ORF clone of AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
RG211284 | AAK1 (tGFP-tagged) - Human AP2 associated kinase 1 (AAK1) |
CNY 7,890.00 |
|
SC316852 | AAK1 (untagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 10,568.00 |