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MED9 (NM_018019) Human Tagged ORF Clone
CAT＃: RC211226
MED9 (Myc-DDK-tagged)-Human mediator complex subunit 9 (MED9)
ORF Plasmid: tGFP
"NM_018019" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MED25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211226 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTGCTGGGGTGGCAGCCGGGCGACAGGCGGAGGATGTATTGCCGCCAACGTCCGACCAGCCGC TGCCTGACACCAAGCCGCTGCCGCCTCCTCAGCCGCCGCCGGTCCCTGCGCCTCAACCGCAGCAGTCGCC GGCGCCACGGCCTCAGTCACCTGCCCGCGCGAGGGAGGAAGAGAACTACTCCTTTTTACCTTTGGTTCAC AACATCATCAAATGCATGGACAAGGACAGCCCGGAGGTCCACCAGGACCTGAACGCCCTCAAAAGCAAGT TCCAGGAGATGCGCAAGCTCATCAGCACCATGCCCGGCATCCACCTGAGCCCCGAACAGCAGCAGCAGCA GCTGCAGAGCCTCCGGGAGCAAGTCAGGACCAAGAATGAGCTTCTGCAAAAGTACAAGAGCCTCTGCATG TTCGAAATCCCCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211226 protein sequence
Red=Cloning site Green=Tags(s) MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQQSPAPRPQSPARAREEENYSFLPLVH NIIKCMDKDSPEVHQDLNALKSKFQEMRKLISTMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCM FEIPKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018019 |
| ORF Size | 438 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018019.3 |
| RefSeq Size | 2222 bp |
| RefSeq ORF | 441 bp |
| Locus ID | 55090 |
| UniProt ID | Q9NWA0 |
| MW | 16.4 kDa |
| Gene Summary | The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211226L3 | Lenti ORF clone of Human mediator complex subunit 9 (MED9), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211226L4 | Lenti ORF clone of Human mediator complex subunit 9 (MED9), mGFP tagged |
CNY 5,890.00 |
|
| RG211226 | MED9 (tGFP-tagged) - Human mediator complex subunit 9 (MED9) |
CNY 2,800.00 |
|
| SC113804 | MED9 (untagged)-Human mediator complex subunit 9 (MED9) |
CNY 3,990.00 |
