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GATA4 (NM_002052) Human Tagged ORF Clone
CAT＃: RC210945
GATA4 (Myc-DDK-tagged)-Human GATA binding protein 4 (GATA4)
ORF Plasmid: tGFP
"NM_002052" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASD2; TACHD; TOF; VSD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210945 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCAGAGCTTGGCCATGGCCGCCAACCACGGGCCGCCCCCCGGTGCCTACGAGGCGGGCGGCCCCG GCGCCTTCATGCACGGCGCGGGCGCCGCGTCCTCGCCAGTCTACGTGCCCACACCGCGGGTGCCCTCCTC CGTGCTGGGCCTGTCCTACCTCCAGGGCGGAGGCGCGGGCTCTGCGTCCGGAGGCGCCTCGGGCGGCAGC TCCGGTGGGGCCGCGTCTGGTGCGGGGCCCGGGACCCAGCAGGGCAGCCCGGGATGGAGCCAGGCGGGAG CCGACGGAGCCGCTTACACCCCGCCGCCGGTGTCGCCGCGCTTCTCCTTCCCGGGGACCACCGGGTCCCT GGCGGCCGCCGCCGCCGCTGCCGCGGCCCGGGAAGCTGCGGCCTACAGCAGTGGCGGCGGAGCGGCGGGT GCGGGCCTGGCGGGCCGCGAGCAGTACGGGCGCGCCGGCTTCGCGGGCTCCTACTCCAGCCCCTACCCGG CTTACATGGCCGACGTGGGCGCGTCCTGGGCCGCAGCCGCCGCCGCCTCCGCCGGCCCCTTCGACAGCCC GGTCCTGCACAGCCTGCCCGGCCGGGCCAACCCGGCCGCCCGACACCCCAATCTCGATATGTTTGACGAC TTCTCAGAAGGCAGAGAGTGTGTCAACTGTGGGGCTATGTCCACCCCGCTCTGGAGGCGAGATGGGACGG GTCACTATCTGTGCAACGCCTGCGGCCTCTACCACAAGATGAACGGCATCAACCGGCCGCTCATCAAGCC TCAGCGCCGGCTGTCCGCCTCCCGCCGAGTGGGCCTCTCCTGTGCCAACTGCCAGACCACCACCACCACG CTGTGGCGCCGCAATGCGGAGGGCGAGCCTGTGTGCAATGCCTGCGGCCTCTACATGAAGCTCCACGGGG TCCCCAGGCCTCTTGCAATGCGGAAAGAGGGGATCCAAACCAGAAAACGGAAGCCCAAGAACCTGAATAA ATCTAAGACACCAGCAGCTCCTTCAGGCAGTGAGAGCCTTCCTCCCGCCAGCGGTGCTTCCAGCAACTCC AGCAACGCCACCACCAGCAGCAGCGAGGAGATGCGTCCCATCAAGACGGAGCCTGGCCTGTCATCTCACT ACGGGCACAGCAGCTCCGTGTCCCAGACGTTCTCAGTCAGTGCGATGTCTGGCCATGGGCCCTCCATCCA CCCTGTCCTCTCGGCCCTGAAGCTCTCCCCACAAGGCTATGCGTCTCCCGTCAGCCAGTCTCCACAGACC AGCTCCAAGCAGGACTCTTGGAACAGCCTGGTCTTGGCCGACAGTCACGGGGACATAATCACTGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210945 protein sequence
Red=Cloning site Green=Tags(s) MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGS SGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAG AGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDD FSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTT LWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNS SNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQT SSKQDSWNSLVLADSHGDIITA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002052 |
| ORF Size | 1326 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002052.5 |
| RefSeq Size | 3419 bp |
| RefSeq ORF | 1329 bp |
| Locus ID | 2626 |
| UniProt ID | P43694 |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 44.6 kDa |
| Gene Summary | This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Deciphering Transcriptional Networks during Human Cardiac Development
,null,
Cells
,PubMed ID 36497174
[GATA4]
|
|
Analysis of variants in GATA4 and FOG2/ZFPM2 demonstrates benign contribution to 46,XY disorders of sex development
,van den Bergen, JA;Robevska, G;Eggers, S;Riedl, S;Grover, SR;Bergman, PB;Kimber, C;Jiwane, A;Khan, S;Krausz, C;Raza, J;Atta, I;Davis, SR;Ono, M;Harley, V;Faradz, SMH;Sinclair, AH;Ayers, KL;,
Mol Genet Genomic Med
,PubMed ID 31962012
[GATA4]
|
|
A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts
,Xie, B;Sun, D;Du, Y;Jia, J;Sun, S;Xu, J;Liu, Y;Xiang, C;Chen, S;Xie, H;Wang, Q;Li, G;Lyu, X;Shen, H;Li, S;Wu, M;Zhang, X;Pu, Y;Xiang, K;Lai, W;Du, P;Yuan, Z;Li, C;Shi, Y;Lu, S;Deng, H;,
Cell Res.
,PubMed ID 31270412
[GATA4]
|
|
Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition
,Gao, L;Hu, Y;Tian, Y;Fan, Z;Wang, K;Li, H;Zhou, Q;Zeng, G;Hu, X;Yu, L;Zhou, S;Tong, X;Huang, H;Chen, H;Liu, Q;Liu, W;Zhang, G;Zeng, M;Zhou, G;He, Q;Ji, H;Chen, L;,
Nat Commun
,PubMed ID 30971692
[GATA4]
|
|
The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
,Chae, YJ;Jun, DW;Lee, JS;Saeed, WK;Kang, HT;Jang, K;Lee, JH;,
Gut Liver
,PubMed ID 30602218
[GATA4]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210945L1 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210945L2 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), mGFP tagged |
CNY 6,056.00 |
|
| RC210945L3 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210945L4 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), mGFP tagged |
CNY 6,056.00 |
|
| RG210945 | GATA4 (tGFP-tagged) - Human GATA binding protein 4 (GATA4) |
CNY 5,256.00 |
|
| SC124037 | GATA4 (untagged)-Human GATA binding protein 4 (GATA4) |
CNY 3,656.00 |
