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DMT1 (SLC11A2) (NM_000617) Human Tagged ORF Clone
CAT＃: RC210568
SLC11A2 (Myc-DDK-tagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4
ORF Plasmid: tGFP
"NM_000617" in other vectors (6)
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CNY 4,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AHMIO1; DCT1; DMT1; NRAMP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGGTCCTGAACAGAAGATGTCAGATGACAGTGTTTCTGGAGATCATGGGGAGTCTGCCAGTC TTGGTAACATCAACCCTGCCTATAGTAATCCCTCTCTTTCACAGTCCCCTGGGGACTCAGAGGAGTACTT CGCCACTTACTTTAATGAGAAGATCTCCATTCCTGAGGAGGAGTACTCTTGTTTTAGCTTTCGTAAACTC TGGGCTTTCACCGGACCAGGTTTTCTTATGAGCATTGCCTACCTGGATCCAGGAAATATTGAATCCGATT TGCAGTCTGGAGCAGTGGCTGGATTTAAGTTGCTCTGGATCCTTCTGTTGGCCACCCTTGTGGGGCTGCT GCTCAAGCGGCTTGCAGCTAGACTGGGAGTGGTTACTGGGCTGCATCTTGCTGAAGTATGTCACCGTCAG TATCCCAAGGTCCCACGAGTCATCCTGTGGCTGATGGTGGAGTTGGCTATCATCGGCTCAGACATGCAAG AAGTCATTGGCTCAGCCATTGCTATCAATCTTCTGTCTGTAGGAAGAATTCCTCTGTGGGGTGGCGTTCT CATCACCATTGCAGATACTTTTGTATTTCTCTTCTTGGACAAATATGGCTTGCGGAAGCTAGAAGCATTT TTTGGCTTTCTCATCACTATTATGGCCCTCACATTTGGATATGAGTATGTTACAGTGAAACCCAGCCAGA GCCAGGTACTCAAGGGCATGTTCGTACCATCCTGTTCAGGCTGTCGCACTCCACAGATTGAACAGGCTGT GGGCATCGTGGGAGCTGTCATCATGCCACACAACATGTACCTGCATTCTGCCTTAGTCAAGTCTAGACAG GTAAACCGGAACAATAAGCAGGAAGTTCGAGAAGCCAATAAGTACTTTTTCATTGAATCCTGCATTGCAC TCTTTGTTTCCTTCATCATCAATGTCTTTGTTGTCTCAGTCTTTGCTGAAGCATTTTTTGGGAAAACCAA CGAGCAGGTGGTTGAAGTCTGTACAAATACCAGCAGTCCTCATGCTGGCCTCTTTCCTAAAGATAACTCG ACACTGGCTGTGGACATCTACAAAGGGGGTGTTGTGCTGGGATGTTACTTTGGGCCTGCTGCACTCTACA TTTGGGCAGTGGGGATCCTGGCTGCAGGACAGAGCTCCACCATGACAGGAACCTATTCTGGCCAGTTTGT CATGGAGGGATTCCTGAACCTAAAGTGGTCACGCTTTGCCCGAGTGGTTCTGACTCGCTCTATTGCCATC ATCCCCACTCTGCTTGTTGCTGTCTTCCAAGATGTAGAGCATCTAACAGGGATGAATGACTTTCTGAATG TTCTACAGAGCTTACAGCTTCCCTTTGCTCTCATACCCATCCTCACATTTACGAGCTTGCGGCCAGTAAT GAGTGACTTTGCCAATGGACTAGGCTGGCGGATTGCAGGAGGAATCTTGGTCCTTATCATCTGTTCCATC AATATGTACTTTGTAGTGGTTTATGTCCGGGACCTAGGGCATGTGGCATTATATGTGGTGGCTGCTGTGG TCAGCGTGGCTTATCTGGGCTTTGTGTTCTACTTGGGTTGGCAATGTTTGATTGCACTGGGCATGTCCTT CCTGGACTGTGGGCATACGGTAAGCATCTCTAAAGGCCTGCTGACAGAAGAAGCCACCCGTGGCTACGTT AAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210568 protein sequence
Red=Cloning site Green=Tags(s) MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKL WAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLKRLAARLGVVTGLHLAEVCHRQ YPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAF FGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQ VNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNS TLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAI IPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSI NMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTVSISKGLLTEEATRGYV K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000617 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000617.3 |
| RefSeq Size | 4164 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 4891 |
| UniProt ID | P49281 |
| Domains | Nramp |
| Protein Families | Transmembrane |
| Protein Pathways | Lysosome |
| MW | 61.5 kDa |
| Gene Summary | This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210568L1 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, Myc-DDK-tagged |
CNY 6,576.00 |
|
| RC210568L2 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RC210568L3 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210568L4 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, mGFP tagged |
CNY 6,576.00 |
|
| RG210568 | SLC11A2 (tGFP-tagged) - Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4 |
CNY 5,776.00 |
|
| SC119791 | SLC11A2 (untagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4 |
CNY 4,184.00 |
