USP21 (NM_012475) Human Tagged ORF Clone
CAT#: RC210554
USP21 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 21 (USP21), transcript variant 1
ORF Plasmid: tGFP
"NM_012475" in other vectors (6)
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CNY 4,208.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | USP16; USP23 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210554 representing NM_012475
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCAGGCCTCTGAGCACCGCCTGGGCCGTACCCGAGAGCCACCTGTTAATATCCAGCCCCGAGTGG GATCCAAGCTACCATTTGCCCCCAGGGCCCGCAGCAAGGAGCGCAGAAACCCAGGCTCTGGGCCAAACCC CATGTTACGACCTCTGCCTCCCCGGCCAGGTCTGCCTGATGAACGGCTCAAGAAACTGGAGCTGGGACGG GGACGGACCTCAGGCCCTCGTCCCAGAGGCCCCCTTCGAGCAGATCATGGGGTTCCCCTGCCTGGCTCAC CACCCCCAACAGTGGCTTTGCCTCTCCCATCTCGGACCAACTTAGCCCGTTCCAAGTCTGTGAGCAGTGG GGACTTGCGTCCAATGGGGATTGCCTTGGGAGGGCACCGTGGCACCGGAGAGCTTGGGGCTGCACTGAGC CGCTTGGCCCTCCGGCCTGAGCCACCCACTTTGAGACGTAGCACTTCTCTCCGCCGCCTAGGGGGCTTTC CTGGACCCCCTACCCTGTTCAGCATACGGACAGAGCCCCCTGCTTCCCATGGCTCCTTCCACATGATATC CGCCCGGTCCTCTGAGCCTTTCTACTCTGATGACAAGATGGCTCATCACACACTCCTTCTGGGCTCTGGT CATGTTGGCCTTCGAAACCTGGGAAACACGTGCTTCCTGAATGCTGTGCTGCAGTGTCTGAGCAGCACTC GACCTCTTCGGGACTTCTGTCTGAGAAGGGACTTCCGGCAAGAGGTGCCTGGAGGAGGCCGAGCCCAAGA GCTCACTGAAGCCTTTGCAGATGTGATTGGTGCCCTCTGGCACCCTGACTCCTGCGAAGCTGTGAATCCT ACTCGATTCCGAGCTGTCTTCCAGAAATATGTTCCCTCCTTCTCTGGATACAGCCAGCAGGATGCCCAAG AGTTCCTGAAGCTCCTCATGGAGCGGCTACACCTTGAAATCAACCGCCGAGGCCGCCGGGCTCCACCGAT ACTTGCCAATGGTCCAGTTCCCTCTCCACCCCGCCGAGGAGGGGCTCTGCTAGAAGAACCTGAGTTAAGT GATGATGACCGAGCCAACCTAATGTGGAAACGTTACCTGGAGCGAGAGGACAGCAAGATTGTGGACCTGT TTGTGGGCCAGTTGAAAAGTTGTCTCAAGTGCCAGGCCTGTGGGTATCGCTCCACGACCTTCGAGGTTTT TTGTGACCTGTCCCTGCCCATCCCCAAGAAAGGATTTGCTGGGGGCAAGGTGTCTCTGCGGGATTGTTTC AACCTTTTCACTAAGGAAGAAGAGCTAGAGTCGGAGAATGCCCCAGTGTGTGACCGATGTCGGCAGAAAA CTCGAAGTACCAAAAAGTTGACAGTACAAAGATTCCCTCGAATCCTCGTGCTCCATCTGAATCGATTTTC TGCCTCCCGAGGCTCCATCAAAAAAAGTTCAGTAGGTGTAGACTTTCCACTGCAGCGACTGAGCCTAGGG GACTTTGCCAGTGACAAAGCCGGAAGTCCTGTATACCAGCTGTATGCCCTTTGCAACCACTCAGGCAGCG TCCACTATGGCCACTACACAGCCCTGTGCCGGTGCCAGACTGGTTGGCATGTCTACAATGACTCTCGTGT CTCCCCTGTCAGTGAAAACCAGGTGGCATCCAGCGAGGGCTACGTGCTGTTCTACCAACTGATGCAGGAG CCACCCCGGTGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210554 representing NM_012475
Red=Cloning site Green=Tags(s) MPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPGSGPNPMLRPLPPRPGLPDERLKKLELGR GRTSGPRPRGPLRADHGVPLPGSPPPTVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALS RLALRPEPPTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSG HVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNP TRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELS DDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCF NLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLG DFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQE PPRCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012475 |
ORF Size | 1695 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012475.5 |
RefSeq Size | 2217 bp |
RefSeq ORF | 1698 bp |
Locus ID | 27005 |
UniProt ID | Q9UK80 |
Domains | UCH |
Protein Families | Druggable Genome, Protease |
MW | 62.5 kDa |
Gene Summary | This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Ablation of USP21 in skeletal muscle promotes oxidative fibre phenotype, inhibiting obesity and type 2 diabetes) Ablation of USP21 in skeletal muscle promotes oxidative fibre phenotype, inhibiting obesity and type 2 diabetes
,null,
Journal of Cachexia, Sarcopenia and Muscle
,PubMed ID 34523817
[USP21]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210554L1 | Lenti ORF clone of Human ubiquitin specific peptidase 21 (USP21), transcript variant 1, Myc-DDK-tagged |
CNY 6,608.00 |
|
RC210554L2 | Lenti ORF clone of Human ubiquitin specific peptidase 21 (USP21), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC210554L3 | Lenti ORF clone of Human ubiquitin specific peptidase 21 (USP21), transcript variant 1, Myc-DDK-tagged |
CNY 6,608.00 |
|
RC210554L4 | Lenti ORF clone of Human ubiquitin specific peptidase 21 (USP21), transcript variant 1, mGFP tagged |
CNY 6,608.00 |
|
RG210554 | USP21 (tGFP-tagged) - Human ubiquitin specific peptidase 21 (USP21), transcript variant 1 |
CNY 4,370.00 |
|
SC126539 | USP21 (untagged)-Human ubiquitin specific peptidase 21 (USP21), transcript variant 1 |
CNY 4,216.00 |