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GSDME (NM_004403) Human Tagged ORF Clone
CAT＃: RC210515
DFNA5 (Myc-DDK-tagged)-Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1
ORF Plasmid: tGFP
"NM_004403" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNA5; ICERE-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210515 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGCCAAAGCAACCAGGAATTTTCTTAGAGAAGTTGATGCTGATGGTGACCTGATTGCAGTATCAA ATCTGAATGACTCTGATAAGTTACAGCTTCTAAGTCTGGTGACAAAAAAGAAGAGATTCTGGTGCTGGCA GAGACCCAAGTACCAGTTTTTATCCCTCACCCTTGGCGATGTACTCATAGAAGACCAATTTCCGAGTCCA GTGGTCGTGGAGTCGGACTTTGTGAAATACGAGGGCAAGTTTGCAAACCACGTGAGTGGAACCCTGGAGA CTGCACTGGGGAAGGTCAAGCTGAACCTGGGGGGCAGCAGCCGCGTAGAGAGCCAGTCTTCATTTGGAAC CCTGAGGAAGCAGGAGGTGGATTTGCAGCAGCTCATCAGAGACTCTGCCGAGAGAACAATAAATCTGAGA AACCCTGTGCTCCAGCAGGTGCTGGAGGGAAGGAATGAGGTCCTGTGCGTTTTGACACAGAAGATCACGA CGATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAAGTGTGGTGGCATCGTGGGCATCCA GACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGGGAATGTCACCAAGGACTCCAACGTGGTGCTGGAG ATCCCAGCTGCCACCACCATTGCCTACGGTGTCATTGAGTTATACGTGAAACTGGACGGCCTGTTCGAGT TCTGCCTTCTCCGAGGGAAGCAAGGTGGCTTCGAGAACAAGAAGAGAATTGACTCTGTCTACCTGGACCA CCTGGTCTTTCGAGAGTTTGCATTCATAGACATGCCAGATGCTGCGCATGGGATATCTTCCCAGGATGGA CCATTAAGTGTTTTAAAGCAAGCGACCCTGCTCCTGGAGAGGAATTTCCATCCATTTGCGGAGCTGCCTG AGCCACAACAGACAGCTTTGAGTGACATCTTCCAGGCGGTCGTATTTGATGATGAACTACTCATGGTCCT GGAACCAGTGTGCGATGACCTGGTCAGCGGCCTCTCGCCCACAGTGGCGGTGCTGGGGGAGCTGAAGCCC CGGCAGCAGCAGGACCTTGTGGCCTTCCTGCAGCTGGTGGGGTGCAGCTTACAGGGTGGGTGTCCGGGCC CCGAGGATGCAGGCAGCAAGCAGCTGTTTATGACAGCCTACTTCTTGGTCAGTGCCCTCGCAGAAATGCC AGATAGCGCAGCAGCTCTGCTGGGCACTTGCTGCAAACTCCAGATCATTCCCACACTGTGCCACTTGCTT CGTGCTCTGTCTGATGATGGAGTATCTGATCTTGAAGACCCAACCTTGACTCCCCTGAAAGATACAGAAA GGTTTGGGATTGTGCAGCGCTTGTTTGCCTCAGCTGACATTAGTCTGGAGAGACTGAAGTCATCTGTGAA AGCTGTCATTCTGAAGGACTCTAAAGTCTTCCCACTGCTTCTTTGTATAACCCTGAATGGACTCTGTGCT TTAGGCAGAGAACATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210515 protein sequence
Red=Cloning site Green=Tags(s) MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSP VVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLR NPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLE IPAATTIAYGVIELYVKLDGLFEFCLLRGKQGGFENKKRIDSVYLDHLVFREFAFIDMPDAAHGISSQDG PLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVVFDDELLMVLEPVCDDLVSGLSPTVAVLGELKP RQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLL RALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCA LGREHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004403 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004403.3 |
| RefSeq Size | 2521 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 1687 |
| UniProt ID | O60443 |
| Domains | DFNA5 |
| Protein Families | Druggable Genome |
| MW | 54.6 kDa |
| Gene Summary | Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Gasdermin E is required for induction of pyroptosis and severe disease during enterovirus 71 infection
,null,
The Journal of Biological Chemistry
,PubMed ID 35339492
[GSDME]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210515L1 | Lenti ORF clone of Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210515L2 | Lenti ORF clone of Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210515L3 | Lenti ORF clone of Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210515L4 | Lenti ORF clone of Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210515 | DFNA5 (tGFP-tagged) - Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1 |
CNY 5,256.00 |
|
| SC117401 | DFNA5 (untagged)-Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1 |
CNY 3,656.00 |
