TLR3 (NM_003265) Human Tagged ORF Clone
CAT#: RC210497
- TrueORF®
TLR3 (Myc-DDK-tagged)-Human toll-like receptor 3 (TLR3)
ORF Plasmid: tGFP
"NM_003265" in other vectors (6)
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CNY 6,624.00
CNY 7,130.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CD283; IIAE2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210497 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACAGACTTTGCCTTGTATCTACTTTTGGGGGGGCCTTTTGCCCTTTGGGATGCTGTGTGCATCCT CCACCACCAAGTGCACTGTTAGCCATGAAGTTGCTGACTGCAGCCACCTGAAGTTGACTCAGGTACCCGA TGATCTACCCACAAACATAACAGTGTTGAACCTTACCCATAATCAACTCAGAAGATTACCAGCCGCCAAC TTCACAAGGTATAGCCAGCTAACTAGCTTGGATGTAGGATTTAACACCATCTCAAAACTGGAGCCAGAAT TGTGCCAGAAACTTCCCATGTTAAAAGTTTTGAACCTCCAGCACAATGAGCTATCTCAACTTTCTGATAA AACCTTTGCCTTCTGCACGAATTTGACTGAACTCCATCTCATGTCCAACTCAATCCAGAAAATTAAAAAT AATCCCTTTGTCAAGCAGAAGAATTTAATCACATTAGATCTGTCTCATAATGGCTTGTCATCTACAAAAT TAGGAACTCAGGTTCAGCTGGAAAATCTCCAAGAGCTTCTATTATCAAACAATAAAATTCAAGCGCTAAA AAGTGAAGAACTGGATATCTTTGCCAATTCATCTTTAAAAAAATTAGAGTTGTCATCGAATCAAATTAAA GAGTTTTCTCCAGGGTGTTTTCACGCAATTGGAAGATTATTTGGCCTCTTTCTGAACAATGTCCAGCTGG GTCCCAGCCTTACAGAGAAGCTATGTTTGGAATTAGCAAACACAAGCATTCGGAATCTGTCTCTGAGTAA CAGCCAGCTGTCCACCACCAGCAATACAACTTTCTTGGGACTAAAGTGGACAAATCTCACTATGCTCGAT CTTTCCTACAACAACTTAAATGTGGTTGGTAACGATTCCTTTGCTTGGCTTCCACAACTAGAATATTTCT TCCTAGAGTATAATAATATACAGCATTTGTTTTCTCACTCTTTGCACGGGCTTTTCAATGTGAGGTACCT GAATTTGAAACGGTCTTTTACTAAACAAAGTATTTCCCTTGCCTCACTCCCCAAGATTGATGATTTTTCT TTTCAGTGGCTAAAATGTTTGGAGCACCTTAACATGGAAGATAATGATATTCCAGGCATAAAAAGCAATA TGTTCACAGGATTGATAAACCTGAAATACTTAAGTCTATCCAACTCCTTTACAAGTTTGCGAACTTTGAC AAATGAAACATTTGTATCACTTGCTCATTCTCCCTTACACATACTCAACCTAACCAAGAATAAAATCTCA AAAATAGAGAGTGATGCTTTCTCTTGGTTGGGCCACCTAGAAGTACTTGACCTGGGCCTTAATGAAATTG GGCAAGAACTCACAGGCCAGGAATGGAGAGGTCTAGAAAATATTTTCGAAATCTATCTTTCCTACAACAA GTACCTGCAGCTGACTAGGAACTCCTTTGCCTTGGTCCCAAGCCTTCAACGACTGATGCTCCGAAGGGTG GCCCTTAAAAATGTGGATAGCTCTCCTTCACCATTCCAGCCTCTTCGTAACTTGACCATTCTGGATCTAA GCAACAACAACATAGCCAACATAAATGATGACATGTTGGAGGGTCTTGAGAAACTAGAAATTCTCGATTT GCAGCATAACAACTTAGCACGGCTCTGGAAACACGCAAACCCTGGTGGTCCCATTTATTTCCTAAAGGGT CTGTCTCACCTCCACATCCTTAACTTGGAGTCCAACGGCTTTAACGAGATCCCAGTTGAGGTCTTCAAGG ATTTATTTGAACTAAAGATCATCGATTTAGGATTGAATAATTTAAACACACTTCCAGCATCTGTCTTTAA TAATCAGGTGTCTCTAAAGTCATTGAACCTTCAGAAGAATCTCATAACATCCGTTGAGAAGAAGGTTTTC GGGCCAGCTTTCAGGAACCTGACTGAGTTAGATATGCGCTTTAATCCCTTTGATTGCACGTGTGAAAGTA TTGCCTGGTTTGTTAATTGGATTAACGAGACCCATACCAACATCCCTGAGCTGTCAAGCCACTACCTTTG CAACACTCCACCTCACTATCATGGGTTCCCAGTGAGACTTTTTGATACATCATCTTGCAAAGACAGTGCC CCCTTTGAACTCTTTTTCATGATCAATACCAGTATCCTGTTGATTTTTATCTTTATTGTACTTCTCATCC ACTTTGAGGGCTGGAGGATATCTTTTTATTGGAATGTTTCAGTACATCGAGTTCTTGGTTTCAAAGAAAT AGACAGACAGACAGAACAGTTTGAATATGCAGCATATATAATTCATGCCTATAAAGATAAGGATTGGGTC TGGGAACATTTCTCTTCAATGGAAAAGGAAGACCAATCTCTCAAATTTTGTCTGGAAGAAAGGGACTTTG AGGCGGGTGTTTTTGAACTAGAAGCAATTGTTAACAGCATCAAAAGAAGCAGAAAAATTATTTTTGTTAT AACACACCATCTATTAAAAGACCCATTATGCAAAAGATTCAAGGTACATCATGCAGTTCAACAAGCTATT GAACAAAATCTGGATTCCATTATATTGGTTTTCCTTGAGGAGATTCCAGATTATAAACTGAACCATGCAC TCTGTTTGCGAAGAGGAATGTTTAAATCTCACTGCATCTTGAACTGGCCAGTTCAGAAAGAACGGATAGG TGCCTTTCGTCATAAATTGCAAGTAGCACTTGGATCCAAAAACTCTGTACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210497 protein sequence
Red=Cloning site Green=Tags(s) MRQTLPCIYFWGGLLPFGMLCASSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAAN FTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKN NPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIK EFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLD LSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFS FQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKIS KIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRV ALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKG LSHLHILNLESNGFNEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVF GPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSA PFELFFMINTSILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRQTEQFEYAAYIIHAYKDKDWV WEHFSSMEKEDQSLKFCLEERDFEAGVFELEAIVNSIKRSRKIIFVITHHLLKDPLCKRFKVHHAVQQAI EQNLDSIILVFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERIGAFRHKLQVALGSKNSVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003265 |
ORF Size | 2712 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003265.3 |
RefSeq Size | 3057 bp |
RefSeq ORF | 2715 bp |
Locus ID | 7098 |
UniProt ID | O15455 |
Domains | TIR, LRRCT, LRR, LRR_TYP, LRR_PS |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Toll-like receptor signaling pathway |
MW | 103.8 kDa |
Gene Summary | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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Epigenetic state determines inflammatory sensing in neuroblastoma
,Wolpaw, A;Grossmann, L;Dong, M;Dessau, J;Brafford, P;Volgina, D;Rodriguez-Garcia, A;Uzun, Y;Powell, D;Tan, K;Hogarty, M;Maris, J;Dang, C;,
bioRxiv
[TLR3]
|
Identification and characterization of rare toll-like receptor 3 variants in patients with autoimmune Addison's disease
,Aslaksen, S;Wolff, A;Vigeland, M;Breivik, L;Sheng, Y;Oftedal, B;Artaza, H;Skinningsrud, B;Undlien, D;Selmer, K;Husebye, E;Bratland, E;,
Journal of Translational Autoimmunity
[TLR3]
|
Bufalin Suppresses Migration and Invasion of Hepatocellular Carcinoma Cells Elicited by Poly (I:C) Therapy
,Feng, Y;Chen, Y;Meng, Y;Cao, Q;Liu, Q;Ling, C;Wang, C;,
Oncoimmunology
,PubMed ID 29721392
[TLR3]
|
Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210497L1 | Lenti ORF clone of Human toll-like receptor 3 (TLR3), Myc-DDK-tagged |
CNY 9,024.00 |
|
RC210497L2 | Lenti ORF clone of Human toll-like receptor 3 (TLR3), mGFP tagged |
CNY 9,024.00 |
|
RC210497L3 | Lenti ORF clone of Human toll-like receptor 3 (TLR3), Myc-DDK-tagged |
CNY 9,024.00 |
|
RC210497L4 | Lenti ORF clone of Human toll-like receptor 3 (TLR3), mGFP tagged |
CNY 9,024.00 |
|
RG210497 | TLR3 (tGFP-tagged) - Human toll-like receptor 3 (TLR3) |
CNY 8,224.00 |
|
SC112723 | TLR3 (untagged)-Human toll-like receptor 3 (TLR3) |
CNY 6,632.00 |