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Fibrinogen beta chain (FGB) (NM_005141) Human Tagged ORF Clone
CAT＃: RC210471
FGB (Myc-DDK-tagged)-Human fibrinogen beta chain (FGB), transcript variant 1
ORF Plasmid: tGFP
"NM_005141" in other vectors (7)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-78p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGGATGGTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATCTATTATTGCTACTATTGT GTGTTTTTTTAGTTAAGTCCCAAGGTGTCAACGACAATGAGGAGGGTTTCTTCAGTGCCCGTGGTCATCG ACCCCTTGACAAGAAGAGAGAAGAGGCTCCCAGCCTGAGGCCTGCCCCACCGCCCATCAGTGGAGGTGGC TATCGGGCTCGTCCAGCCAAAGCAGCTGCCACTCAAAAGAAAGTAGAAAGAAAAGCCCCTGATGCTGGAG GCTGTCTTCACGCTGACCCAGACCTGGGGGTGTTGTGTCCTACAGGATGTCAGTTGCAAGAGGCTTTGCT ACAACAGGAAAGGCCAATCAGAAATAGTGTTGATGAGTTAAATAACAATGTGGAAGCTGTTTCCCAGACC TCCTCTTCTTCCTTTCAGTACATGTATTTGCTGAAAGACCTGTGGCAAAAGAGGCAGAAGCAAGTAAAAG ATAATGAAAATGTAGTCAATGAGTACTCCTCAGAACTGGAAAAGCACCAATTATATATAGATGAGACTGT GAATAGCAATATCCCAACTAACCTTCGTGTGCTTCGTTCAATCCTGGAAAACCTGAGAAGCAAAATACAA AAGTTAGAATCTGATGTCTCAGCTCAAATGGAATATTGTCGCACCCCATGCACTGTCAGTTGCAATATTC CTGTGGTGTCTGGCAAAGAATGTGAGGAAATTACCAGGAAAGGAGGTGAAACATCTGAAATGTATCTCAT TCAACCTGACAGTTCTGTCAAACCGTATAGAGTATACTGTGACATGAATACAGAAAATGGAGGATGGACA GTGATTCAGAACCGTCAAGACGGTAGTGTTGACTTTGGCAGGAAATGGGATCCATATAAACAGGGATTTG GAAATGTTGCAACCAACACAGATGGGAAGAATTACTGTGGCCTACCAGGTGAATATTGGCTTGGAAATGA TAAAATTAGCCAGCTTACCAGGATGGGACCCACAGAACTTTTGATAGAAATGGAGGACTGGAAAGGAGAC AAAGTAAAGGCTCACTATGGAGGATTCACTGTACAGAATGAAGCCAACAAATACCAGATCTCAGTGAACA AATACAGAGGAACAGCCGGTAATGCCCTCATGGATGGAGCATCTCAGCTGATGGGAGAAAACAGGACCAT GACCATTCACAACGGCATGTTCTTCAGCACGTATGACAGAGACAATGACGGCTGGTTAACATCAGATCCC AGAAAACAGTGTTCTAAAGAAGACGGTGGTGGATGGTGGTATAATAGATGTCATGCAGCCAATCCAAACG GCAGATACTACTGGGGTGGACAGTACACCTGGGACATGGCAAAGCATGGCACAGATGATGGTGTAGTATG GATGAATTGGAAGGGGTCATGGTACTCAATGAGGAAGATGAGTATGAAGATCAGGCCCTTCTTCCCACAG CAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210471 protein sequence
Red=Cloning site Green=Tags(s) MKRMVSWSFHKLKTMKHLLLLLLCVFLVKSQGVNDNEEGFFSARGHRPLDKKREEAPSLRPAPPPISGGG YRARPAKAAATQKKVERKAPDAGGCLHADPDLGVLCPTGCQLQEALLQQERPIRNSVDELNNNVEAVSQT SSSSFQYMYLLKDLWQKRQKQVKDNENVVNEYSSELEKHQLYIDETVNSNIPTNLRVLRSILENLRSKIQ KLESDVSAQMEYCRTPCTVSCNIPVVSGKECEEITRKGGETSEMYLIQPDSSVKPYRVYCDMNTENGGWT VIQNRQDGSVDFGRKWDPYKQGFGNVATNTDGKNYCGLPGEYWLGNDKISQLTRMGPTELLIEMEDWKGD KVKAHYGGFTVQNEANKYQISVNKYRGTAGNALMDGASQLMGENRTMTIHNGMFFSTYDRDNDGWLTSDP RKQCSKEDGGGWWYNRCHAANPNGRYYWGGQYTWDMAKHGTDDGVVWMNWKGSWYSMRKMSMKIRPFFPQ Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005141 |
| ORF Size | 1473 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005141.5 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 2244 |
| UniProt ID | P02675 |
| Domains | FBG |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades |
| MW | 55.9 kDa |
| Gene Summary | The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Fibrinogen serves key roles in hemostasis and antimicrobial host defense. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq, Aug 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210471L1 | Lenti ORF clone of Human fibrinogen beta chain (FGB), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210471L2 | Lenti ORF clone of Human fibrinogen beta chain (FGB), transcript variant 1, mGFP tagged |
CNY 6,180.00 |
|
| RC210471L3 | Lenti ORF clone of Human fibrinogen beta chain (FGB), transcript variant 1, Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC210471L4 | Lenti ORF clone of Human fibrinogen beta chain (FGB), transcript variant 1, mGFP tagged |
CNY 6,180.00 |
|
| RG210471 | FGB (tGFP-tagged) - Human fibrinogen beta chain (FGB), transcript variant 1 |
CNY 5,256.00 |
|
| SC112673 | FGB (untagged)-Human fibrinogen beta chain (FGB), transcript variant 1 |
CNY 3,656.00 |
|
| SC321032 | FGB (untagged)-Human fibrinogen beta chain (FGB), transcript variant 1 |
CNY 3,656.00 |
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