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NOX1 (NM_007052) Human Tagged ORF Clone
CAT＃: RC210426
NOX1 (Myc-DDK-tagged)-Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L
ORF Plasmid: tGFP
"NM_007052" in other vectors (6)
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CNY 6,296.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GP91-2; MOX1; NOH-1; NOH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210426 representing NM_007052
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACTGGGTGGTTAACCACTGGTTTTCAGTTTTGTTTCTGGTTGTTTGGTTAGGGCTGAATGTTT TCCTGTTTGTGGATGCCTTCCTGAAATATGAGAAGGCCGACAAATACTACTACACAAGAAAAATCCTTGG GTCAACATTGGCCTGTGCCCGAGCGTCTGCTCTCTGCTTGAATTTTAACAGCACGCTGATCCTGCTTCCT GTGTGTCGCAATCTGCTGTCCTTCCTGAGGGGCACCTGCTCATTTTGCAGCCGCACACTGAGAAAGCAAT TGGATCACAACCTCACCTTCCACAAGCTGGTGGCCTATATGATCTGCCTACATACAGCTATTCACATCAT TGCACACCTGTTTAACTTTGACTGCTATAGCAGAAGCCGACAGGCCACAGATGGCTCCCTTGCCTCCATT CTCTCCAGCCTATCTCATGATGAGAAAAAGGGGGGTTCTTGGCTAAATCCCATCCAGTCCCGAAACACGA CAGTGGAGTATGTGACATTCACCAGCATTGCTGGTCTCACTGGAGTGATCATGACAATAGCCTTGATTCT CATGGTAACTTCAGCTACTGAGTTCATCCGGAGGAGTTATTTTGAAGTCTTCTGGTATACTCACCACCTT TTTATCTTCTATATCCTTGGCTTAGGGATTCACGGCATTGGTGGAATTGTCCGGGGTCAAACAGAGGAGA GCATGAATGAGAGTCATCCTCGCAAGTGTGCAGAGTCTTTTGAGATGTGGGATGATCGTGACTCTCACTG TAGGCGCCCTAAGTTTGAAGGGCATCCCCCTGAGTCTTGGAAGTGGATCCTTGCACCGGTCATTCTTTAT ATCTGTGAAAGGATCCTCCGGTTTTACCGCTCCCAGCAGAAGGTTGTGATTACCAAGGTTGTTATGCACC CATCCAAAGTTTTGGAATTGCAGATGAACAAGCGTGGCTTCAGCATGGAAGTGGGGCAGTATATCTTTGT TAATTGCCCCTCAATCTCTCTCCTGGAATGGCATCCTTTTACTTTGACCTCTGCTCCAGAGGAAGATTTC TTCTCCATTCATATCCGAGCAGCAGGGGACTGGACAGAAAATCTCATAAGGGCTTTCGAACAACAATATT CACCAATTCCCAGGATTGAAGTGGATGGTCCCTTTGGCACAGCCAGTGAGGATGTTTTCCAGTATGAAGT GGCTGTGCTGGTTGGAGCAGGAATTGGGGTCACCCCCTTTGCTTCTATCTTGAAATCCATCTGGTACAAA TTCCAGTGTGCAGACCACAACCTCAAAACAAAAAAGATCTATTTCTACTGGATCTGCAGGGAGACAGGTG CCTTTTCCTGGTTCAACAACCTGTTGACTTCCCTGGAACAGGAGATGGAGGAATTAGGCAAAGTGGGTTT TCTAAACTACCGTCTCTTCCTCACCGGATGGGACAGCAATATTGTTGGTCATGCAGCATTAAACTTTGAC AAGGCCACTGACATCGTGACAGGTCTGAAACAGAAAACCTCCTTTGGGAGACCAATGTGGGACAATGAGT TTTCTACAATAGCTACCTCCCACCCCAAGTCTGTAGTGGGAGTTTTCTTATGTGGCCCTCGGACTTTGGC AAAGAGCCTGCGCAAATGCTGTCACCGATATTCCAGTCTGGATCCTAGAAAGGTTCAATTCTACTTCAAC AAAGAAAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210426 representing NM_007052
Red=Cloning site Green=Tags(s) MGNWVVNHWFSVLFLVVWLGLNVFLFVDAFLKYEKADKYYYTRKILGSTLACARASALCLNFNSTLILLP VCRNLLSFLRGTCSFCSRTLRKQLDHNLTFHKLVAYMICLHTAIHIIAHLFNFDCYSRSRQATDGSLASI LSSLSHDEKKGGSWLNPIQSRNTTVEYVTFTSIAGLTGVIMTIALILMVTSATEFIRRSYFEVFWYTHHL FIFYILGLGIHGIGGIVRGQTEESMNESHPRKCAESFEMWDDRDSHCRRPKFEGHPPESWKWILAPVILY ICERILRFYRSQQKVVITKVVMHPSKVLELQMNKRGFSMEVGQYIFVNCPSISLLEWHPFTLTSAPEEDF FSIHIRAAGDWTENLIRAFEQQYSPIPRIEVDGPFGTASEDVFQYEVAVLVGAGIGVTPFASILKSIWYK FQCADHNLKTKKIYFYWICRETGAFSWFNNLLTSLEQEMEELGKVGFLNYRLFLTGWDSNIVGHAALNFD KATDIVTGLKQKTSFGRPMWDNEFSTIATSHPKSVVGVFLCGPRTLAKSLRKCCHRYSSLDPRKVQFYFN KENF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007052 |
| ORF Size | 1692 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007052.5 |
| RefSeq Size | 2612 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 27035 |
| UniProt ID | Q9Y5S8 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Protein Pathways | Leukocyte transendothelial migration |
| MW | 64.7 kDa |
| Gene Summary | This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2
,null,
Cold Spring Harbor Molecular Case Studies
,PubMed ID 30709874
[NOX1]
|
|
Interleukin-4 and interleukin-13 increase NADPH oxidase 1-related proliferation of human colon cancer cells
,Liu, H;Antony, S;Roy, K;Juhasz, A;Wu, Y;Lu, J;Meitzler, JL;Jiang, G;Polley, E;Doroshow, JH;,
Oncotarget
,PubMed ID 28498822
[NOX1]
|
|
CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activity
,Prior, KK;Leisegang, MS;Josipovic, I;Löwe, O;Shah, AM;Weissmann, N;Schröder, K;Brandes, RP;,
REDOX BIOLOGY
,PubMed ID 27614387
[Nox1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210426L1 | Lenti ORF clone of Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L, Myc-DDK-tagged |
CNY 8,696.00 |
|
| RC210426L2 | Lenti ORF clone of Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L, mGFP tagged |
CNY 5,890.00 |
|
| RC210426L3 | Lenti ORF clone of Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L, Myc-DDK-tagged |
CNY 8,696.00 |
|
| RC210426L4 | Lenti ORF clone of Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L, mGFP tagged |
CNY 8,696.00 |
|
| RG210426 | NOX1 (tGFP-tagged) - Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L |
CNY 7,896.00 |
|
| SC123944 | NOX1 (untagged)-Human NADPH oxidase 1 (NOX1), transcript variant NOH-1L |
CNY 6,312.00 |
