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Myostatin Propeptide (MSTN) (NM_005259) Human Tagged ORF Clone
CAT＃: RC210368
MSTN (Myc-DDK-tagged)-Human myostatin (MSTN)
ORF Plasmid: tGFP
"NM_005259" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDF8; MSLHP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210368 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAACTGCAACTCTGTGTTTATATTTACCTGTTTATGCTGATTGTTGCTGGTCCAGTGGATCTAA ATGAGAACAGTGAGCAAAAAGAAAATGTGGAAAAAGAGGGGCTGTGTAATGCATGTACTTGGAGACAAAA CACTAAATCTTCAAGAATAGAAGCCATTAAGATACAAATCCTCAGTAAACTTCGTCTGGAAACAGCTCCT AACATCAGCAAAGATGTTATAAGACAACTTTTACCCAAAGCTCCTCCACTCCGGGAACTGATTGATCAGT ATGATGTCCAGAGGGATGACAGCAGCGATGGCTCTTTGGAAGATGACGATTATCACGCTACAACGGAAAC AATCATTACCATGCCTACAGAGTCTGATTTTCTAATGCAAGTGGATGGAAAACCCAAATGTTGCTTCTTT AAATTTAGCTCTAAAATACAATACAATAAAGTAGTAAAGGCCCAACTATGGATATATTTGAGACCCGTCG AGACTCCTACAACAGTGTTTGTGCAAATCCTGAGACTCATCAAACCTATGAAAGACGGTACAAGGTATAC TGGAATCCGATCTCTGAAACTTGACATGAACCCAGGCACTGGTATTTGGCAGAGCATTGATGTGAAGACA GTGTTGCAAAATTGGCTCAAACAACCTGAATCCAACTTAGGCATTGAAATAAAAGCTTTAGATGAGAATG GTCATGATCTTGCTGTAACCTTCCCAGGACCAGGAGAAGATGGGCTGAATCCGTTTTTAGAGGTCAAGGT AACAGACACACCAAAAAGATCCAGAAGGGATTTTGGTCTTGACTGTGATGAGCACTCAACAGAATCACGA TGCTGTCGTTACCCTCTAACTGTGGATTTTGAAGCTTTTGGATGGGATTGGATTATCGCTCCTAAAAGAT ATAAGGCCAATTACTGCTCTGGAGAGTGTGAATTTGTATTTTTACAAAAATATCCTCATACTCATCTGGT ACACCAAGCAAACCCCAGAGGTTCAGCAGGCCCTTGCTGTACTCCCACAAAGATGTCTCCAATTAATATG CTATATTTTAATGGCAAAGAACAAATAATATATGGGAAAATTCCAGCGATGGTAGTAGACCGCTGTGGGT GCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210368 protein sequence
Red=Cloning site Green=Tags(s) MQKLQLCVYIYLFMLIVAGPVDLNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAP NISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFF KFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKT VLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESR CCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINM LYFNGKEQIIYGKIPAMVVDRCGCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005259 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005259.3 |
| RefSeq Size | 2823 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 2660 |
| UniProt ID | O14793 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 42.8 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210368L1 | Lenti ORF clone of Human myostatin (MSTN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210368L2 | Lenti ORF clone of Human myostatin (MSTN), mGFP tagged |
CNY 5,890.00 |
|
| RC210368L3 | Lenti ORF clone of Human myostatin (MSTN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210368L4 | Lenti ORF clone of Human myostatin (MSTN), mGFP tagged |
CNY 6,056.00 |
|
| RG210368 | MSTN (tGFP-tagged) - Human myostatin (MSTN) |
CNY 5,256.00 |
|
| SC124056 | MSTN (untagged)-Human myostatin (MSTN) |
CNY 3,656.00 |
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