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IER5 (NM_016545) Human Tagged ORF Clone
CAT＃: RC210318
IER5 (Myc-DDK-tagged)-Human immediate early response 5 (IER5)
ORF Plasmid: tGFP
"NM_016545" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SBBI48 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210318 representing NM_016545
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCAAGCTGGAGGCTCATCGCATCGTCAGCATCTCTCTGGGCAAGATCTACAACTCGCGGGTCC AGCGCGGCGGCATCAAGCTGCATAAGAACCTCCTGGTCTCGCTGGTGCTGCGCAGCGCCCGCCAAGTCTA CCTGAGCGACCCGTGCCCCGGCCTCTACCTGGCCGGTCCCGCTGGGACCCCGGCGCCGCCACCGCAGCAG CAGCCCGGGGAGCCGGCGGCCGGGCCACCCGCCGGCTGGGGAGAGCCGCCCCCGCCCGCCGCTCGTGCCT CTTGGCCGGAGACCGAGCCGCAGCCGGAGCGCTCCTCCGTCTCAGACGCGCCGCGGGTAGGGGACGAGGT GCCGGTGGCCACGGTGACTGGAGTCGGGGACGTTTTTCAGGGCGGAGAGGCGGACGCGACGGAAGCTGCC TGGAGCCGCGTGGAGGGGCCGCGCCAGGCGGCGGCCAGAGAAGCCGAGGGTACCGCCGGAGGCTGGGGCG TCTTCCCCGAGGTATCTCGTGCCGCGCGCCGCCCCTGCGGCTGCCCCCTAGGCGGGGAGGACCCGCCGGG TACACCGGCCGCGACCCCCCGCGCTGCCTGCTGCTGCGCGCCGCGACCAGCGGAGGACGAGCCCCCCGCG CCGCCCGCGGTGTGCCCCAGGAAGCGCTGCGCGGCGGGGGTGGGCGGCGGCCCAGCGGGCTGCCCGGCGC CCGGCTCGACCCCGCTCAAGAAGCCCCGCCGGAACTTAGAGCAGCCGCCGAGTGGAGGAGAGGACGACGA CGCGGAGGAGATGGAGACCGGGAACGTGGCTAACCTCATCAGCATCTTCGGTTCCAGTTTCTCGGGACTC CTACGGAAAAGCCCCGGGGGCGGCAGAGAGGAAGAGGAGGGAGAGGAGAGCGGTCCGGAAGCCGCCGAGC CCGGGCAGATCTGCTGCGATAAGCCGGTGCTGAGAGACATGAACCCCTGGAGCACAGCCATCGTGGCCTT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210318 representing NM_016545
Red=Cloning site Green=Tags(s) MEFKLEAHRIVSISLGKIYNSRVQRGGIKLHKNLLVSLVLRSARQVYLSDPCPGLYLAGPAGTPAPPPQQ QPGEPAAGPPAGWGEPPPPAARASWPETEPQPERSSVSDAPRVGDEVPVATVTGVGDVFQGGEADATEAA WSRVEGPRQAAAREAEGTAGGWGVFPEVSRAARRPCGCPLGGEDPPGTPAATPRAACCCAPRPAEDEPPA PPAVCPRKRCAAGVGGGPAGCPAPGSTPLKKPRRNLEQPPSGGEDDDAEEMETGNVANLISIFGSSFSGL LRKSPGGGREEEEGEESGPEAAEPGQICCDKPVLRDMNPWSTAIVAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016545 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016545.3, NP_057629.1 |
| RefSeq Size | 2369 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 51278 |
| UniProt ID | Q5VY09 |
| MW | 33.5 kDa |
| Gene Summary | This gene encodes a protein that is similar to other immediate early response proteins. In the mouse, a similar gene may play an important role in mediating the cellular response to mitogenic signals. Studies in rats found the expression of a similar gene to be increased after waking and sleep deprivation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210318L1 | Lenti ORF clone of Human immediate early response 5 (IER5), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210318L2 | Lenti ORF clone of Human immediate early response 5 (IER5), mGFP tagged |
CNY 5,890.00 |
|
| RC210318L3 | Lenti ORF clone of Human immediate early response 5 (IER5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210318L4 | Lenti ORF clone of Human immediate early response 5 (IER5), mGFP tagged |
CNY 5,890.00 |
|
| RG210318 | IER5 (tGFP-tagged) - Human immediate early response 5 (IER5) |
CNY 4,000.00 |
|
| SC107946 | IER5 (untagged)-Human immediate early response 5 (IER5) |
CNY 2,400.00 |
